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Characterization of Indian bred rose cultivars using morphological and molecular markers for conservation and sustainable management

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Abstract

Rose (Rosa × hybrid L.) is one of the most important commercial ornamental crops cultivated worldwide for its beauty, fragrance and nutraceutical values. Characterization of rose germplasm provides precise information about the extent of diversity present among the cultivars. It also helps in cultivar identification, intellectual property right protection, variety improvement and genetic diversity conservation. In the present study, 109 Indian bred rose cultivars were characterized using 59 morphological and 48 SSR markers. Out of 48 SSRs used, 31 markers exhibited polymorphism and 96 alleles were identified with an average of 3.9 alleles per locus. Nei’s expected heterozygosity value of each locus ranged from 0.08 (with SSR ABRII/RPU32) to 0.78 (SSR Rh58). The similarity coefficient values ranged from 0.42 to 0.90 which indicated presence of moderated diversity among Indian cultivars. The neighbor-joining tree based on morphological data grouped the cultivars into two major clusters and several minor clusters based on their morphological resemblance. However, UPGMA dendrogram constructed using matching coefficient values grouped the cultivars into eight different clusters. Interpopulation analysis revealed higher genetic similarities between Hybrid Tea and Floribunda cultivars. An analysis for presence of population sub-structure grouped the Indian cultivars into eight different genetic groups. Analysis of molecular variance revealed apportioning of 97.59% of the variation to within subgroup diversity and 3.07% to between the cultivar groups. We have demonstrated here successful utilization of robust SSR to distinguish cultivars and assess genetic diversity among Indian bred rose cultivars. The information provided here is useful for cultivar identification and protection, cultivar improvement and genetic diversity conservation.

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Acknowledgements

The authors thank the Indian Council of Agricultural Research for the fellowship to the first author (AV) and for the facilities provided for conducting this work. The views expressed in this article are authors own and do not reflect the official position of the Indian Council of Agricultural Research.

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Authors and Affiliations

  1. Division of Floriculture and Landscape Architecture, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India

    Aparna Veluru, Namita Banyal, Kanwar Pal Singh & Sapna Panwar

  2. ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, 110 012, India

    Kangila Venkataramana Bhat

  3. Assistant Director General, Horticulture, KAB-II, Indian Council of Agricultural Research, New Delhi, 110 012, India

    Janakiram Tolety

  4. ICAR-Directorate of Floriculture Research, Pune, Maharashtra, 411005, India

    Dantuluri Venkata Sai Raju & Kuchimanchi Venkata Prasad

  5. Division of Genetics, ICAR- Indian Agricultural Research Institute, New Delhi, 110 012, India

    Chellapilla Bharadwaj

  6. ICAR-National Research Centre on Plant Biotechnology, New Delhi, 110 012, India

    Sevanthi Venkata Amitha Charu Rama Mitra

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  1. Aparna Veluru
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Correspondence to Kangila Venkataramana Bhat.

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Veluru, A., Bhat, K.V., Raju, D.V.S. et al. Characterization of Indian bred rose cultivars using morphological and molecular markers for conservation and sustainable management. Physiol Mol Biol Plants 26, 95–106 (2020). https://doi.org/10.1007/s12298-019-00735-8

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  • DOI: https://doi.org/10.1007/s12298-019-00735-8

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