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The polygalacturonase-inhibiting protein 4 (OsPGIP4), a potential component of the qBlsr5a locus, confers resistance to bacterial leaf streak in rice

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Abstract

Main conclusion

OsPGIP4 overexpression enhances resistance to bacterial leaf streak in rice.

Polygalacturonase-inhibiting proteins are thought to play important roles in the innate immunity of rice against fungi. Here, we show that the chromosomal location of OsPGIP4 coincides with the major bacterial leaf streak resistance quantitative trait locus qBlsr5a on the short arm of chromosome 5. OsPGIP4 expression was up-regulated upon inoculation with the pathogen Xanthomonas oryzae pv. oryzicola strain RS105. OsPGIP4 overexpression enhanced the resistance of the susceptible rice variety Zhonghua 11 to RS105. In contrast, repressing OsPGIP4 expression resulted in an increase in disease lesions caused by RS105 in Zhonghua 11 and in Acc8558, a qBlsr5a resistance donor. More interestingly, upon inoculation, the activated expression of pathogenesis-related genes was attenuated for those genes involved in the salicylic acid pathway, while the activated expression of jasmonic acid pathway markers was increased in the overexpression lines. Our results not only provide the first report that rice PGIP could enhance resistant against a bacterial pathogen but also indicate that OsPGIP4 is a potential component of the qBlsr5a locus for bacterial leaf streak in rice.

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Abbreviations

BB:

Bacterial blight

BLS:

Bacterial leaf streak

DR:

Defense-related

JA:

Jasmonic acid

OGs:

Oligogalacturonides

PG:

Polygalacturonase

PGIP:

Polygalacturonase-inhibiting protein

R:

Resistance

SA:

Salicylic acid

Xoc:

Xanthomonas oryzae pv. oryzicola

Xoo:

Xanthomonas oryzae pv. oryzae

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Acknowledgments

This study was supported by the National Program of Transgenic Variety Development of China (2013ZX08009-004, 2014ZX08001-002), Foundation for the Author of National Excellent Doctoral Dissertation of PR China (Grant No. 201132), Shandong Provincial Natural Science Foundation, China (ZR2015CM004). Z.C. was funded by the Taishan Scholar Program of Shandong Province and by the Shandong Modern Agricultural Technology & Industry System.

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    Authors and Affiliations

    1. State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Tai’an, 271018, Shandong, People’s Republic of China

      Chuanshun Feng, Xia Zhang, Tao Wu, Xinhua Ding & Zhaohui Chu

    2. Institute of Plant Protection and Soil Fertilizer, Hubei Academy of Agricultural Sciences, Wuhan, 430064, Hubei, People’s Republic of China

      Bin Yuan

    3. Biotechnology Research Center, Shandong Academy of Agricultural Science, Jinan, 250100, Shandong, People’s Republic of China

      Fangying Yao

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    1. Chuanshun Feng
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    Correspondence to Xinhua Ding or Zhaohui Chu.

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    C. Feng and X. Zhang contributed equally to this work.

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    425_2016_2480_MOESM1_ESM.pdf

    Suppl. Fig. S1 The inhibition of PG activity for OsPGIP4 overexpression rice. Three individual OsPGIP4 overexpression lines were performed for the inhibition of PG activity that compared with the wild type ZH11. Suppl. Fig. S2 The PG homologs of Xanthomonas oryzae. Alignment of PG homologs in the Xoo strains PXO99A and PXO86 and in the Xoc strains BLS256 and RS105. The substitution amino acids are highlighted in bold. Suppl. Fig. S3 The lesion areas to PXO99 for OsPGIP4 overexpression and RNAi plants. Lesion areas were counted by the ratio between the lesion length and leaf length after inoculation with the Xoo strain PXO99 in T1 generation. Each line included at least 5 positive individuals for the experiment (PDF 74 kb)

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    Feng, C., Zhang, X., Wu, T. et al. The polygalacturonase-inhibiting protein 4 (OsPGIP4), a potential component of the qBlsr5a locus, confers resistance to bacterial leaf streak in rice. Planta 243, 1297–1308 (2016). https://doi.org/10.1007/s00425-016-2480-z

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