Abstract.
In this study, an outwardly rectifying anion channel was characterized in the cell line K562 obtained from a chronic human leukaemia. Ion channel activity was recorded in the cell-detached (inside-out) configuration with standard patch-clamp technology. In most of the K562 cells studied, the channel exhibited low spontaneous activity, an outwardly rectifying current/voltage relationship and single-channel conductances of 19 pS and 40 pS for inwards and outwards currents respectively. The channel had a low permeability for gluconate with a relative permeability P gluconate/P Cl of 0.14 and was blocked by glibenclamide (50 µM) or diphenylamine-2-carboxylate (DPC, 1 mM) added to the cytoplasmic side of the patch. These results are characteristic of the outwardly rectifying Cl channel (ORCC) found in other types of cells.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Electronic Publication
Rights and permissions
About this article
Cite this article
Assef, Y.A., Kotsias, B.A. An outwardly rectifying anion channel in human leukaemic K562 cells. Pflugers Arch - Eur J Physiol 444, 816–820 (2002). https://doi.org/10.1007/s00424-002-0891-0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s00424-002-0891-0