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Altered Expression of Auxin-binding Protein 1 Affects Cell Expansion and Auxin Pool Size in Tobacco Cells

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Abstract

Auxin-binding protein 1 (ABP1) has an essential role in auxin-dependent cell expansion, but its mechanisms of action remain unknown. Our previous study showed that ABP1-mediated cell expansion is auxin concentration dependent. However, auxin distribution in plant tissue is heterogeneous, complicating the interpretation of ABP1 function. In this study, we used cells in culture that have altered expression of ABP1 to address the mechanism of ABP1 action at the cellular level, because cells in culture have homogeneous cell types and could potentially circumvent the heterogeneous auxin-distributions inherent in plant tissues. We found that cells overexpressing ABP1 had altered sensitivity to auxin and were larger, with nuclei that have undergone endoreduplication, a finding consistent with other data that support an auxin extracellular receptor role for ABP1. These cells also had a higher free auxin pool size, which cannot be explained by altered auxin transport. In cells lacking detectable ABP1, a higher rate of auxin metabolism was observed. The results suggest that ABP1 has, beyond its proposed role as an auxin extracellular receptor, a role in mediating auxin availability.

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Acknowledgments

This work was supported by grants from the U.S. National Science Foundation, Integrative Plant Biology Program; U.S. National Institute of General Medical Sciences (NIGMS), and the U.S. Department of Agriculture, National Research Initiative Competitive Grants Program (NRICGP), Plant Growth and Development Program to A.M.J.

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  1. Jin-Gui Chen & Shucai Wang

    Present address: Department of Botany, University of British Columbia, V6T 1Z4, Vancouver, British Columbia, Canada

Authors and Affiliations

  1. Department of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, 27599-3280, USA

    Jin-Gui Chen & Alan M. Jones

  2. Department of Agronomy, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Shucai Wang

  3. School of Biological Sciences, University of Bristol, Bristol, BS8 1UG, United Kingdom

    Colin M. Lazarus

  4. Horticulture Research International, Wellesbourne, Warwick, CV35 9EF, United Kingdom

    Richard M. Napier

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  1. Jin-Gui Chen
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  2. Shucai Wang
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Correspondence to Jin-Gui Chen.

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Chen, JG., Wang, S., Lazarus, C.M. et al. Altered Expression of Auxin-binding Protein 1 Affects Cell Expansion and Auxin Pool Size in Tobacco Cells. J Plant Growth Regul 25, 69–78 (2006). https://doi.org/10.1007/s00344-005-0049-3

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  • DOI: https://doi.org/10.1007/s00344-005-0049-3

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