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Uptake, accumulation and metabolism of auxins in tobacco leaf protoplasts

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Abstract

Uptake and metabolism of exogenous naphthalene-1-acetic acid (NAA) and indole-3-acetic acid (IAA) have been studied in tobacco (Nicotiana tabacum L. cv. Xanthi) mesophyll protoplasts. Both auxins entered protoplasts by diffusion under the action of the transmembrane pH gradient without any detectable participation of an influx carrier. Molecules were accumulated by an anion-trapping mechanism and most of them were metabolized within hours, essentially as glucose-ester and amino-acid conjugates. Protoplasts were equipped with a functional auxin-efflux carrier as evidenced by the inhibitory effect of naphthylphtalamic acid on IAA efflux. Basically, similar mechanisms of NAA and IAA uptake occurred in protoplasts. However, the two auxins differed in their levels of accumulation, due to different membrane-transport characteristics, and the nature of the metabolites produced. This shows the need to estimate the accumulation and the metabolism of auxins when analyzing their effects in a given cell system. The internal auxin concentration could be modulated by changing the transmembrane pH gradient, giving an interesting perspective for discriminating between the effects of intra- and extracellular auxin on physiological processes.

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Abbreviations

BA:

benzoic acid

Ci/Ce :

accumulation ratio of auxin

IAAasp:

N-[3-indolylacetyl]-dl-aspartic acid

NAA:

naphthalene-1-acetic acid

NAAasp:

N-[1-naphthylacetyl]-l-aspartic acid

NPA:

N-1-naphthylphthalamic acid

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Authors and Affiliations

  1. Institut des Sciences Végétales, CNRS Bâtiment 22, Avenue de la Terrasse, F-91198, Gif-sur-Yvette, France

    Alain Delbarre, Philippe Muller, Viviane Imhoff & Hélène Barbier-Brygoo

  2. CRBA, Faculté de Pharmacie, Université Montpellier I, 15 Avenue Charles Flahaut, F-34060, Montpellier, France

    Jean-Louis Morgat

Authors
  1. Alain Delbarre
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  2. Philippe Muller
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  3. Viviane Imhoff
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  4. Jean-Louis Morgat
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  5. Hélène Barbier-Brygoo
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Additional information

The authors thank Dr. M. Caboche (I.N.R.A, Versailles, France) for his generous gifts of some amide derivatives of 1-NAA, Mr. P. Varennes and Dr. B. Das (I.C.S.N., C.N.R.S., Gif-sur-Yvette, France) for recording and interpreting the mass spectra of NAA glucose ester, and Prof. P. Manigault (Institut des Sciences Végétales, Gif-sur-Yvette) for microscopy measurements of protoplast dimensions. This work was supported by funds from the C.N.R.S, I.N.R.A, and E.E.C.

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Delbarre, A., Muller, P., Imhoff, V. et al. Uptake, accumulation and metabolism of auxins in tobacco leaf protoplasts. Planta 195, 159–167 (1994). https://doi.org/10.1007/BF00199674

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  • DOI: https://doi.org/10.1007/BF00199674

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