Abstract
The contents of indole-3-acetic acid (IAA), gibberellins (GAs), abscisic acid (ABA), and cytokinins were determined in ovules of normal cotton (Tm-1) and a kind of fiber differentiation mutant (Xin) before and after flowering by enzyme-linked immunosorbent assays. It was found that 24 h before flowering, a peak of IAA content was observed in ovules of Tm-1, whereas in ovules of Xin, a low level of IAA was determined. From −1 day (1 day before flowering) to +3 days (3 days after flowering), GA1+3 levels in ovules of Xin were 40–70% lower than those of Tm-1; GA4+7 levels were very low, and there was no visible difference in GA4+7 content between normal and mutant cotton. The ABA content in ovule of Tm-1 decreased by 70% 3 days after flowering, whereas that of Xin only decreased by 20%. The levels of cytokinins in ovules of Tm-1 decreased after flowering, and those of Xin kept up a steady increase.
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Abbreviations
- IAA:
-
indole-3-acetic acid
- GA:
-
gibberellin
- ABA:
-
abscisic acid
- ELISA:
-
enzyme-linked immunosorbent assay
- FW:
-
fresh weight
- PBS:
-
phosphate-buffered saline
- iPA:
-
isopentenyladenosine
- ZR:
-
zeatin riboside
- DHZR:
-
dihydrozeatin riboside
- CTK:
-
cytokinin
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Chen, JG., Du, XM., Zhao, HY. et al. Fluctuation in levels of endogenous plant hormones in ovules of normal and mutant cotton during flowering and their relation to fiber development. J Plant Growth Regul 15, 173–177 (1996). https://doi.org/10.1007/BF00190581
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DOI: https://doi.org/10.1007/BF00190581