Abstract
Key message
MeRAVs positively regulate ROS burst and the expression of downstream disease resistance-related genes, which underlie improved disease resistance to Xam.
Abstract
Cassava (Manihot esculenta Crantz) is an important food crop and energy crop, but its yield is seriously affected by cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam). Related to ABI3/VP1 (RAV) transcription factor family belongs to the APETALA2/Ethylene-Responsive Factor (AP2/ERF) family, which plays an important role in plant growth, development and response to biotic and abiotic stresses. In this study, we found that MeRAVs positively co-regulates the resistance to Xam and stimulates the innate immune response by regulating reactive oxygen species (ROS) burst in cassava. Dual-luciferase assay showed that seven MeRAVs exhibited transcriptional activate activity by binding CAACA motif and CACCTG motif. A large number of differentially expressed genes (DEGs) were identified through RNA-seq analysis of MeRAVs-silenced lines, and the DEGs co-regulated by seven MeRAVs accounted for more than 45% of the total DEGs. In addition, seven MeRAVs positively regulate expression of disease resistance-related genes through directly binding to their promoters. In summary, MeRAVs co-regulate ROS burst and the expression of downstream disease resistance-related genes, which underlie improved disease resistance to Xam.
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Data availability statement
The raw RNA-seq data were deposited in the NCBI Sequence Read Archive database under accession number PRJNA744150.
Data analysis
The data of each group were displayed by Means ± SD, and there were three biological replicates in each experimental group. Asterisks indicate statistically significant differences (*p < 0.05), determined by Student ttest.
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Acknowledgements
We thank the lab members for field help and sample harvest.
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This study was supported by Hainan Provincial Natural Science Foundation of China (No. 320RC490) and the National Natural Science Foundation of China (No. 32160437 and No. 31960527).
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HS conceived and designed the experiments. PW and GL prepared the plant materials. PW, YY, YL and GL performed the experiments and analyzed the data. PW, YY and JL wrote the manuscript. JL and HS revised the manuscript. All authors read and approved the final manuscript.
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Wang, P., Yan, Y., Lu, Y. et al. The co-modulation of RAV transcription factors in ROS burst and extensive transcriptional reprogramming underlies disease resistance in cassava. Plant Cell Rep 41, 1261–1272 (2022). https://doi.org/10.1007/s00299-022-02855-0
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DOI: https://doi.org/10.1007/s00299-022-02855-0