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MusaATAF2-like protein regulates shoot development and multiplication by inducing cytokinin hypersensitivity and flavonoid accumulation in banana plants

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Abstract

Key message

Senescence-associated transcription factor ATAF2 regulates cytokinin signalling and in vitro shoot multiplication in banana plants.

Abstract

MusaATAF2-like protein is a stress-related NAC transcription factor of banana. It regulates senescence in rooted banana plants. During the early stages of plant development under in vitro conditions, the presence of 6-benzylaminopurine leads to vigorous shoot multiplication. The major contributor to plant shoot multiplication is auxin to cytokinin ratio and their signalling components. The LC–MS analysis of transgenic banana plants overexpressing MusaATAF2 indicated significantly higher cytokinin content and remarkably lower auxin content. Auxin transport has been reported to be inhibited by flavonoids. Their significantly higher abundance in the shoot tissues in transgenic lines suggested potential negative regulation of auxin signalling in transgenic plants. Enhanced shoot multiplication in transgenic lines was further corroborated by reduced transcript abundance of type-A Arabidopsis response regulator-like genes (inhibitors of cytokinin signalling pathway) and higher expression of Arabidopsis histidine kinase-like genes and type-B Arabidopsis response regulator-like genes (positive regulators of cytokinin signalling pathway) in transgenic lines. Altogether, the data concludes that MusaATAF2 induces cytokinin hypersensitivity in banana shoots by modulating/regulating the cytokinin signalling components and flavonoids content.

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Data availability

The complete coding sequence of MusaATAF2 has been deposited in NCBI database as a gene bank accession: MZ467048.

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Acknowledgements

The work was supported from the funding of Department of Atomic Energy, Government of India. SN thanks “Department of Science and Technology” (DST), New Delhi for DST INSPIRE faculty award.

Funding

The work was supported from the funding of Department of Atomic Energy, Government of India. SN thanks “Department of Science and Technology” (DST), New Delhi for DST INSPIRE faculty award.

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Authors and Affiliations

  1. Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, India

    Subham Bhakta, Himanshu Tak & T. R. Ganapathi

  2. Homi Bhabha National Institute, Mumbai, India

    Subham Bhakta, Himanshu Tak, Sudhir Singh & T. R. Ganapathi

  3. Plant Biotechnology and Secondary Metabolites Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, India

    Sudhir Singh

  4. Department of Biotechnology, University of Mumbai, Mumbai, India

    Sanjana Negi

Authors
  1. Subham Bhakta
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  2. Sanjana Negi
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  3. Himanshu Tak
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  4. Sudhir Singh
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  5. T. R. Ganapathi
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Contributions

SB, SN, HT, SS and TG planned and designed the experiments. SB, SN and HT performed the experiments and analysed the data. SB, HT, SS and TG wrote the manuscript. All the authors have read and approved the manuscript. All authors read and approved the manuscript.

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Correspondence to T. R. Ganapathi.

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Communicated by Xian Sheng Zhang.

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Bhakta, S., Negi, S., Tak, H. et al. MusaATAF2-like protein regulates shoot development and multiplication by inducing cytokinin hypersensitivity and flavonoid accumulation in banana plants. Plant Cell Rep 41, 1197–1208 (2022). https://doi.org/10.1007/s00299-022-02849-y

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  • DOI: https://doi.org/10.1007/s00299-022-02849-y

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