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Overexpression of MusaSNAC1 improves shoot proliferation in transgenic banana lines

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Abstract

Augmenting shoot multiplication through genetic engineering is an emerging biotechnological application desirable in optimizing regeneration of genetically modified plants on selection medium and rapid clonal propagation of elite cultivars. Here, we report the improved shoot multiplication in transgenic banana lines with overexpression of MusaSNAC1, a drought-associated NAC transcription factor in banana. Overexpression of MusaSNAC1 induces hypersensitivity of transgenic banana lines toward 6-benzylaminopurine ensuing higher shoot number on different concentrations of 6-benzylaminopurine. Altered transcript levels of multiple genes involved in auxin signaling (Aux/IAA and ARFs) and cytokinin signaling pathways (ARRs) in banana plants overexpressing MusaSNAC1 corroborate the hypersensitivity of transgenic banana plants toward 6-benzylaminopurine. Modulation in expression of ARRs reported to be involved in ABA-hypersensitivity and closure of stomatal aperture correlates with the function of MusaSNAC1 as a drought-responsive NAC transcription factor. Present study suggests a prospective cross talk between shoot multiplication and drought responses coordinated by MusaSNAC1 in banana plants.

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Acknowledgements

Authors thank, Head, Nuclear Agriculture and Biotechnology Division, BARC for support and encouragement. SN thanks “Department of Science and Technology” (DST), New Delhi for DST INSPIRE Faculty award.

Funding

The work was supported from funding of Department of Atomic Energy, Government of India.

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Author notes

  1. Sanjana Negi and Himanshu Tak contributed equally for this study.

Authors and Affiliations

  1. Department of Biotechnology, University of Mumbai, Mumbai, 400098, India

    Sanjana Negi

  2. Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India

    Himanshu Tak & T. R. Ganapathi

  3. Homi Bhabha National Institute, Anushakti Nagar, Mumbai, 400094, India

    Himanshu Tak & T. R. Ganapathi

Authors
  1. Sanjana Negi
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  2. Himanshu Tak
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  3. T. R. Ganapathi
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Contributions

SN, HT, and TG conceived and designed research. SN and HT conducted experiments and analyzed data. SN, HT, and TG wrote the manuscript. All authors read and approved the manuscript.

Corresponding author

Correspondence to T. R. Ganapathi.

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On behalf of all authors, the corresponding author states that there is no conflict of interest.

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Negi, S., Tak, H. & Ganapathi, T.R. Overexpression of MusaSNAC1 improves shoot proliferation in transgenic banana lines. 3 Biotech 11, 188 (2021). https://doi.org/10.1007/s13205-021-02744-5

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