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In vitro conservation of Dendrobium germplasm

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Abstract

Dendrobium is a large genus in the family Orchidaceae that exhibits vast diversity in floral characteristics, which is of considerable importance to orchid breeders, biotechnologists and collectors. Native species have high value as a result of their medicinal properties, while their hybrids are important as ornamental commodities, either as cut flowers or potted plants and are thus veritable industrial crops. Thus, preservation of Dendrobium germplasm is valuable for species conservation, breeding programs and the floriculture industry. Cryopreservation represents the only safe, efficient and cost-effective long-term storage option to facilitate the conservation of genetic resources of plant species. This review highlights 16 years of literature related to the preservation of Dendrobium germplasm and comprises the most comprehensive assessment of thorough studies performed to date, which shows reliable and reproducible results. Air-drying, encapsulation–dehydration, encapsulation–vitrification, vitrification and droplet-vitrification are the current cryopreservation methodologies that have been used to cryopreserve Dendrobium germplasm. Mature seeds, pollen, protoplasts, shoot primordia, protocorms and somatic embryos or protocorm-like bodies (PLBs) have been cryopreserved with different levels of success. Encapsulation–vitrification and encapsulation–dehydration are the most used protocol, while PLBs represent the main explant explored.

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Abbreviations

DMSO:

Dimethyl sulfoxide

FDA:

Fluorescein diacetate

LN:

Liquid nitrogen

PLB:

Protocorm-like body

TTC:

Triphenyl tetrazolium chloride

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Authors and Affiliations

  1. P.O. Box 7, Miki-cho post office 3011-2, Ikenobe, Kagawa-ken, 761-0799, Japan

    Jaime A. Teixeira da Silva

  2. Key Laboratory of South China Agricultural Plant Genetics and Breeding, South China Botanical Garden, Chinese Academy of Sciences, Beijing, 510650, China

    Songjun Zeng

  3. Department of Technology, Universidade Estadual Paulista, Via de Acesso Prof. Dr. Paulo Donato Castellane s/n, Jaboticabal, SP, 14884-900, Brazil

    Renato Fernandes Galdiano Jr.

  4. Research Institute of Nyíregyháza, Research Institutes and Study Farm, Centre for Agricultural and Applied Economic Sciences, University of Debrecen, Nyíregyháza, P.O. Box 12, 4400, Hungary

    Judit Dobránszki

  5. Department of Rural Development, Centro de Ciências Agrárias, UFSCar, Via Anhanguera, km 174, CP 153, Araras, CEP 13.600-970, Brazil

    Jean Carlos Cardoso

  6. Tropical Research and Education Center, University of Florida, 18905 SW 280th St, Homestead, FL, 33031-3314, USA

    Wagner A. Vendrame

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  1. Jaime A. Teixeira da Silva
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  2. Songjun Zeng
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  3. Renato Fernandes Galdiano Jr.
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  4. Judit Dobránszki
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  5. Jean Carlos Cardoso
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  6. Wagner A. Vendrame
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Corresponding authors

Correspondence to Jaime A. Teixeira da Silva, Songjun Zeng, Renato Fernandes Galdiano Jr., Judit Dobránszki, Jean Carlos Cardoso or Wagner A. Vendrame.

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Communicated by N. Stewart.

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Teixeira da Silva, J.A., Zeng, S., Galdiano, R.F. et al. In vitro conservation of Dendrobium germplasm. Plant Cell Rep 33, 1413–1423 (2014). https://doi.org/10.1007/s00299-014-1631-6

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