Skip to main content
SpringerLink
Log in

GISH analysis of disomic Brassica napus-Crambe abyssinica chromosome addition lines produced by microspore culture from monosomic addition lines

  • Genetic Transformation and Hybridization
  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Two Brassica napus--Crambe abyssinica monosomic addition lines (2n=39, AACC plus a single chromosome from C. abyssinca) were obtained from the F2 progeny of the asymmetric somatic hybrid. The alien chromosome from C. abyssinca in the addition line was clearly distinguished by genomic in situ hybridization (GISH). Twenty-seven microspore-derived plants from the addition lines were obtained. Fourteen seedlings were determined to be diploid plants (2n=38) arising from spontaneous chromosome doubling, while 13 seedlings were confirmed as haploid plants. Doubled haploid plants produced after treatment with colchicine and two disomic chromosome addition lines (2n=40, AACC plus a single pair of homologous chromosomes from C. abyssinca) could again be identified by GISH analysis. The lines are potentially useful for molecular genetic analysis of novel C. abyssinica genes or alleles contributing to traits relevant for oilseed rape (B. napus) breeding.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Chen BY, Simonsen V, Lanner-Herrera C, Heneen WK (1992) A Brassia campestric-alboglabra addition line and its use for gene mapping, intergenomic gene transfer and generation of trisomics. Theor Appl Genet 84:592–599

    Google Scholar 

  • Chen JF, Luo XD, Qian CT (2004) Cucumis monosomic alien addition lines: morphological, cytological, and genotypic analyses. Theor Appl Genet 108:1343–1348

    Article  PubMed  Google Scholar 

  • Chévre AM, Eber F, Quiros CF, Delourme R (1994) Creation and characterization of Brassica napus-Diplotaxis erucoides addition lines. Cruciferase Newsl 16:28–29

    Google Scholar 

  • Chuong PV, Beversdorf WD (1985) High frequency embryogenesis through isolated microspore culture in Brassica napus and B.carinata Buaun. Plant Sci 39:219–226

    Article  Google Scholar 

  • Gao D, Guo D, Jung C (2001) Monosomic addition lines of Beta corolliflora Zoss in sugar beet: cytological and molecular-marker analysis. Theor Appl Genet 103:240–247

    Article  CAS  Google Scholar 

  • Gao D, Jung C (2002) Monosomic addition lines of Beta corolliflora in sugar beet: plant morphology and leaf spot resistance. Plant Breed 121:81–86

    Article  Google Scholar 

  • Heslop-Harrison JS, Schwarzacher T (1996) Genomic southern and in situ hybridization for plant genome analysis. In: Jauhar PP (eds) Methods of genome analysis in plants. CRC Press, Boca Raton, FL, pp 163–179

    Google Scholar 

  • Islam AKMR, Shepherd KW (1981) Production of disomic wheat-barley chromosome addition lines using Hordeum bulbosum crosses. Genet Res 37:215–219

    Article  Google Scholar 

  • Jahier J, Chevre AM, Tanguy AM, Eber F (1989) Extraction of disomic addition lines of Brassica napus-B.nigra. Genome 32:408–413

    Google Scholar 

  • Jena KK, Khush GS (1989) Monosomic addition lines of rice: production, morphology, cytology and breeding behavior. Genome 32:449–455

    Google Scholar 

  • Jung C, Koch R, Fischer F (1992) DNA marker closely linked to nematode resistance genes in sugar beet (Beta vulgaris L.) mapped using chromosome additions and translocations origination from wild beets. Mol Gen Genet 232:271–278

    PubMed  CAS  Google Scholar 

  • Kishii M, Yamada T, Sasakuma T, Tsujimoto H (2004) Production of wheat-Leymus racemosus chromosome addition lines. Theor Appl Genet 109:255–260

    Article  PubMed  CAS  Google Scholar 

  • Leelavathi S, Reddy VS, Sen SK (1987) Somatic cell genetic studies in Brassica species. II. Production of androgenetic haploid plants in Brassica nigra (L.) Koch. Euphytica 36:215–219

    Article  Google Scholar 

  • Leino M, Thyselius S, Landgren M, Glimelius K (2004) Arabidopsis thaliana chromosome III restores fertility in a cytoplasmic male-sterile Brassica napus line with A. thaliana mitochondrial DNA. Theor Appl Genet 109:272–279

    Article  PubMed  CAS  Google Scholar 

  • Lichter R (1982) Induction of haploid plants from isolated pollen of Brassica napus. Z PflanzenPhysiol 105:427–434

    Google Scholar 

  • Lichter R (1989) Efficient yield of embryoids by culture of isolated microspores of different Brassicaceae species. Plant Breed 103:119–123

    Article  Google Scholar 

  • Linc G, Schneider A, Molnár I, Molnár-Láng M (2003) Production and molecular cytogenetic identification of Triticum aestivum–Aegilops biuncialis disomic additions. Növénytermelés 52:3–13

    Google Scholar 

  • Lionneton E, Beuret W, Delaitre C, Ochatt S, Rancillac M (2001) Improved microspore culture and doubled-haploid plant regeneration in the brown condiment mustard (Brassica juncea). Plant Cell Rep 20:126–130

    Article  CAS  Google Scholar 

  • Möllers C, Iqbal MCM, Röbbelen G (1994) Efficient production of doubled haploid Brassica napus plants by colchicines treatment of microspores. Euphytica 75:95–104

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:472–493

    Article  Google Scholar 

  • Peffley EB, Corgan JN, Horak KE, Tanksley SD (1985) Electrophoretic analysis of Allium alien addition lines. Theor Appl Genet 71:401–408

    Google Scholar 

  • Peterka H, Budahn H, Schrader O, Ahne R, Schütze W (2004) Transfer of resistance against the beet cyst nematode from radish (Raphanus sativus) to rape (Brassica napus) by monosomic chromosome addition. Theor Appl Genet 109:30–41

    Article  PubMed  CAS  Google Scholar 

  • Rudolf K, Bohanec B, Hansen M (1999) Microspore culture of white cabbage, Brassica oleracea var. capitata L.: Genetic improvement of non-responsive cultivars and effect of genome doubling agent. Plant Breed 118:237–241

    Article  CAS  Google Scholar 

  • Schmidt T, Junghans H, Metzlaff M (1990) Construction of Beta procumbens-specific DNA probes and their application for the screening of B. vulgaris xB. Procumbens (2n=19) addition lines. Theor Appl Genet 79:177–181

    Article  CAS  Google Scholar 

  • Snowdon RJ, Köhler W, Friedt W, Köhler A (1997) Genomic in situ hybridization in Brassica amphidiploids and interspecific hybrids. Theor Appl Genet 95:1320–1324

    Article  CAS  Google Scholar 

  • Snowdon RJ, Winter H, Diestel A, Sacrist`an MD (2000) Development and characterisation of Brassica napus-Sinapis arvensis addition lines exhibiting resistance to Leptosphaeria maculans. Theor Appl Genet 101:1008–1014

    Article  Google Scholar 

  • Suen DF, Wang CK, Lin RF (1997) Assignment of DNA markers to Nicotiana syvestris chromosomes using monosomic alien addition lines. Theor Appl Genet 94:1141–1160

    Article  Google Scholar 

  • Thiagarajah MR, Stringham GR (1993) A comparison of genetic segregation in traditional and microspore-derived populations of Brassica juncea (L.) Czern. and Coss. Plant Breed 117:330–334

    Article  Google Scholar 

  • Wang YP, Tang JS, Chu CQ, Tian J (2000) A preliminary study on the introduction and cultivation of Crambe abyssinica in China, an oil plant for industrial uses. Ind Crops and Prod 12:47–52

    Article  Google Scholar 

  • Wang YP, Sonntag K, Rudloff E (2003) Development of rapeseed with high erucic acid content by asymmetric somatic hybridization between Brassica napus and Crambe abyssinica. Theor Appl Genet 106:1147–1155

    PubMed  CAS  Google Scholar 

  • Wang YP, Snowdon RJ, Rudloff E, Wehling P, Sonntag K (2004) Cytogenetic characterization and fae1 gene variation in progenies from asymmetric somatic hybrids between Brassica napus and Crambe abyssinica. Genome 47:724–731

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported in part by grants from the Fachagentur für Nachwachsende Rohstoffe, Germany (FNR, Grant No. 22014601), and the National Natural Science Foundation of China (Grant No. 30440089).

Author information

Authors and Affiliations

  1. College of Bioscience and Biotechnology, Yangzhou University, 225009, Yangzhou, China

    Youping Wang

  2. Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, 18190, Groß Lüsewitz, Germany

    Karin Sonntag, Eicke Rudloff & Peter Wehling

  3. Institute for Plant Breeding and Crop Science I, Justus Liebig University, Heinrich-Buff-Ring 26–32, 35392, Giessen, Germany

    Rod J. Snowdon

Authors
  1. Youping Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karin Sonntag
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eicke Rudloff
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Peter Wehling
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rod J. Snowdon
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Youping Wang.

Additional information

Communicated by D. Dudits

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, Y., Sonntag, K., Rudloff, E. et al. GISH analysis of disomic Brassica napus-Crambe abyssinica chromosome addition lines produced by microspore culture from monosomic addition lines. Plant Cell Rep 25, 35–40 (2006). https://doi.org/10.1007/s00299-005-0031-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00299-005-0031-3

Keywords

Search

Navigation