Skip to main content
SpringerLink
Log in

Cucumis monosomic alien addition lines: morphological, cytological, and genotypic analyses

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Cucumis hystrix Chakr. (HH, 2n=24), a wild relative of the cultivated cucumber, possesses several potentially valuable disease-resistance and abiotic stress-tolerance traits for cucumber (C. sativus L., CC, 2n=14) improvement. Numerous attempts have been made to transfer desirable traits since the successful interspecific hybridization between C. hystrix and C. sativus, one of which resulted in the production of an allotriploid (HCC, 2n=26: one genome of C. hystrix and two of C. sativus). When this genotype was treated with colchicine to induce polyploidy, two monosomic alien addition lines (MAALs) (plant nos. 87 and 517: 14 CC+1 H, 2n=15) were recovered among 252 viable plants. Each of these plants was morphologically distinct from allotriploids and cultivated cucumbers. Cytogenetic and molecular marker analyses were performed to confirm the genetic constitution and further characterize these two MAALs. Chromosome counts made from at least 30 meristematic cells from each plant confirmed 15 nuclear chromosomes. In pollen mother cells of plant nos. 87 and 517, seven bivalents and one univalent were observed at diakinesis and metaphase I; the frequency of trivalent formation was low (about 4–5%). At anaphase I and II, stochastic and asymmetric division led to the formation of two gamete classes: n=7 and n=8; however, pollen fertility was relatively high. Pollen stainability in plant no. 87 was 86.7% and in plant no. 517 was 93.2%. Random amplified polymorphic DNA analysis was performed using 100 random 10-base primers. Genotypes obtained with eight primers (A-9, A-11, AH-13, AI-19, AJ-18, AJ-20, E-19, and N-20) showed a band common to the two MAAL plants and C. hystrix that was absent in C. sativus, confirming that the alien chromosomes present in the MAALs were derived from C. hystrix. Morphological differences and differences in banding patterns were also observed between plant nos. 87 and 517 after amplification with primers AI-5, AJ-13, N-12, and N-20, suggesting that these plants may contain different C. hystrix chromosomes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1A–C
Fig. 2A–D
Fig. 3A–B

Similar content being viewed by others

References

  • Aung T, Thomas H (1978) The structure and breeding behavior of a translocation involving the transfer of mildew resistance from Avena barbata Pott. into the cultivated oat. Euphytica 27:731–739

    Google Scholar 

  • Brar DS, Khush GS (1997) Alien introgression in rice. Plant Mol Biol 35:35–47

    CAS  PubMed  Google Scholar 

  • Chen BY, Simonsen V, Lanner-Herrera C, Heneen WK (1992) A Brassica campestris-alboglabra addition line and its use for gene mapping, intergenomic gene transfer and generation of trisomics. Theor Appl Genet 84:592–599

    Google Scholar 

  • Chen JF, Kirkbride JH Jr (2000) A new synthetic species Cucumis (Cucurbitaceae) from interspecific hybridization and chromosome doubling. Brittonia 52:315–319

    Google Scholar 

  • Chen JF, Qian ChT (2002) Studies on chromosome preparation using plant tendril as source tissue. Acta Hortic Sin 29:378–380

    Google Scholar 

  • Chen JF, Staub JE, Tashiro Y, Miyazaki S (1997) Successful interspecific hybridization between Cucumis sativus L. and C. hystrix Chakr. Euphytica 96:413–419

    Article  Google Scholar 

  • Chen JF, Luo XD, Staub JE, Jahn MM, Qian ChT, Zhuang FY, Ren G (2003a) An allotriploid derived from a amphidiploid×diploid mating in Cucumis I: production, micropropagation and verification. Euphytica 131:235–241

    Article  CAS  Google Scholar 

  • Chen JF, Staub JE, Qian ChT, Jiang JM, Luo XD, Zhuang FY (2003b) Reproduction and cytogenetic characterization of interspecific hybrids derived from Cucumis hystrix Chakr.×C. sativus L. Theor Appl Genet 106:688-695

    CAS  PubMed  Google Scholar 

  • Deakin JR, Bohn GW, Whitaker TW (1971) Interspecific hybridization in Cucumis. Econ Bot 25:195–211

    Google Scholar 

  • Franken J, Custers JBM, Bino RJ (1988) Effects of temperature on pollen tube growth and fruit set in reciprocal crosses between Cucumis sativus and C. metuliferus. Plant Breed 100:150–153

    Google Scholar 

  • Gao D, Jung C (2002) Monosomic addition lines of Beta corolliflora in sugar beet: plant morphology and leaf spot resistance. Plant Breed 121:81–86

    Article  Google Scholar 

  • Gao D, Guo D, Jung C (2001) Monosomic addition lines of Beta corolliflora Zoss in sugar beet: cytological and molecular-marker analysis. Theor Appl Genet 103:240–247

    Article  CAS  Google Scholar 

  • Jena KK, Khush GS (1989) Monosomic alien addition lines of rice: production, morphology, cytology and breeding behavior. Genome 32:449-455

    Google Scholar 

  • Jung C, Koch R, Fischer F, Brandes A, Wricke G, Herrmann RG (1992) DNA marker closely linked to nematode resistance genes in sugar beer (Beta vulgaris L.) mapped using chromosome additions and translocations origination from wild beets. Mol Gen Genet 232:271–278

    CAS  PubMed  Google Scholar 

  • Kasha KJ (ed) (1974) Haploids from somatic cells, in haploids in higher plants: advances and potential. Proceedings of the First International Symposium on Haploids and Higher Plants. Ainsworth Press, Canada, pp 67–87

  • Khush GS (1973) Cytogenetics of aneuploids. Academic Press, New York

  • Kirkbride JH Jr (1993) Biosystematic monograph of the genus Cucumis (Cucurbitaceae). Parkaway, Boone, NC

  • Knott DR (1961) The inheritance from Agropyron elongatum to common wheat. Can J Plant Sci 41:109–123

    Google Scholar 

  • Kupper RS, Staub JE (1988) Combining ability studies between lines of Cucumis sativus L. and C. sativus var. hardwickii (R.) Alef. Euphytica 38:197–216

    Google Scholar 

  • Leppick EE (1966) Searching gene centers of the genus Cucumis. Euphytica 15:323–328

    Google Scholar 

  • Löptien H (1984) Breeding nematode-resistance beets. II. Investigations into the inheritance of resistance to Heterodera schachtii Schm. in wild species of the section Patellares. Z Pflanzenzuechtg 93:237–245

    Google Scholar 

  • Lower RL, Edwards MD (1986) Cucumber breeding. In: MJ Basset (ed) Breeding vegetable crops. AVI, Westport, Conn., pp 173–207

  • McGrath JM, Quiros CF, Harada JJ, Landry BS (1990) Identification of Brassica oleracea monosomic alien chromosome addition lines with molecular markers reveals extensive gene duplication. Mol Gen Genet 223:198–204

    CAS  PubMed  Google Scholar 

  • Momotaz A, Kato M, Kakihara F (1998) Production of intergeneric hybrids between Brassica and Sinapis species by means of embryo rescue techniques. Euphytica 103:123–130

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Murray HG, Thompson WF (1980) Rapid isolation of higher weight DNA. Nucleic Acids Res 8:4321-4325

    CAS  PubMed  Google Scholar 

  • Nijs APM den, Custers JBM (1990) Introducing resistances into cucumbers by interspecific hybridization. In: Bates DM, Robinson RW, Jeffrey C (eds) Biology and utilization of the Cucurbitaceae. Comstock, Ithaca, NY, pp 382–392

  • O’mara JG (1940) Cytologenetic studies on Triticale. I. A method for determining the effects of individual Secale chromosomes on Triticum. Genetics 25:401–408

    Google Scholar 

  • Peffley EB, Corgan JN, Horak KE, Tanksley SD (1985) Electrophoretic analysis of Allium alien addition lines. Theor Appl Genet 71:401–408

    Google Scholar 

  • Qian CT, Chen JF, Zhuang FY, Xu YB, Li SJ (2002) Several photosynthetic characters of the synthetic species Cucumis hytivus Chen and Kirkbride under weak light condition. Plant Physiol Commun 38:336–338

    CAS  Google Scholar 

  • Sambrook JE, Fritch F, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY

    Google Scholar 

  • Savitsky H (1978) Nematode (Heterodera schachtii) resistance and meiosis in diploid plants from interspecific Beta vulgaris × Beta procumbens hybrids. Can J Genet Cytol 20:177–186

    Google Scholar 

  • Schmidt T, Junghans H, Metzlaff M (1990) Construction of Beta procumbens-specific DNA probes and their application for the screening of B. vulgaris × B. procumbens (2n=19) addition lines. Theor Appl Genet 79:177–181

    CAS  Google Scholar 

  • Sears ER (1956) The transfer of leaf rust resistance from Aegilops umbellulata to wheat. Brookhaven Symp Biol 9:1–22

    Google Scholar 

  • Staub JE, Bacher J, Poetter K (1996) Sources of potential errors in the application of RAPD in cucumber. HortScience 31:262–266

    CAS  Google Scholar 

  • Suen DF, Wang CK, Lin RF, Kao YY, Lee FM, Chen CC (1997) Assignment of DNA markers to Nicotiana sylvestris chromosomes using monosomic alien addition lines. Theor Appl Genet 94:1141–1160

    Article  Google Scholar 

  • Thomas CE (1986) Downy and powdery mildew resistant muskmelon breeding line MR-1. HortScience 21:329

    Google Scholar 

  • Tsuchiya T, Gupta PK (1991) Chromosome engineering in plants: genetics, breeding, evolution. Part B. Elsevier, New York, pp 201–227

    Google Scholar 

  • Zenk MH (1974) Haploids in physiological and biochemical research. In: Kasha KJ (ed) Haploids in higher plants: advances and potential. Guelph University Press, Guelph, Ontario, pp 339–352

  • Zhuang FY, Chen JF, Qian ChT, Li ShJ, Ren G, Wang ZhJ (2002) Responses of seedlings of Cucumis ×hytivus and progenies to low temperature. J Nanjing Agric Univ 25:27–30

    Google Scholar 

Download references

Acknowledgements

This research was partially supported by General Program No. 30170644 from the National Natural Science Foundation of China, by the Department of Education of China (program no. 01097), by National Hi-Tech R and D Program Nos. 2001AA241123 and 2002AA241251, and by Trans-Century Training Program Foundation for the Talents by the State Education Commission, China. Additional support was provided to Dr. Jin-Feng Chen, recipient of the Cornell-China Scholar Award from the Tang Family’s generous gift to Cornell University. Our sincere thanks go to Dr. Hu-Qu Zhai, the President of Chinese Academy of Agricultural Sciences, for his support and to Dr. Hilde Nybom of the Department of Crop Science, Swedish University of Agricultural Sciences for critical review of the manuscript.

Author information

Authors and Affiliations

  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China

    Jin-Feng Chen, Xiang-Dong Luo, Chun-Tao Qian, Fei-Yun Zhuang, Qun-Feng Lou & Gang Ren

  2. Department of Plant Breeding, Cornell University, Ithaca, NY 14853, USA

    Molly M. Jahn

  3. USDA/ARS, Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, WI 53706, USA

    Jack E. Staub

Authors
  1. Jin-Feng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiang-Dong Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chun-Tao Qian
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Molly M. Jahn
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jack E. Staub
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Fei-Yun Zhuang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Qun-Feng Lou
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Gang Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jin-Feng Chen.

Additional information

Communicated by H. Nybom

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, JF., Luo, XD., Qian, CT. et al. Cucumis monosomic alien addition lines: morphological, cytological, and genotypic analyses. Theor Appl Genet 108, 1343–1348 (2004). https://doi.org/10.1007/s00122-003-1546-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-003-1546-z

Keywords

Search

Navigation