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Comparative linkage mapping in the white button mushroom Agaricus bisporus provides foundation for breeding management

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Abstract

To assess the feasibility of marker-assisted selection in mushrooms, a comparative mapping study between two connected populations of the white button mushroom Agaricus bisporus was performed. The first mapping population had been used already for the construction of the A. bisporus reference linkage map. In the present study, a new linkage map based on the segregation analysis of a second generation hybrid progeny was developed. In order to increase the number of available anchor markers, we developed a conversion procedure of an amplified fragment length polymorphism (AFLP) fragment into sequence-specific PCR marker. Seventeen AFLP-converted markers (ACM) were then used for mapping purpose, among which 14 were common to the two maps. The linkage map presented herein consists of 183 markers (53 cleaved amplified polymorphic sequence, 16 SSR, 17 ACM, 96 AFLP and PPC1 locus), distributed among 13 linkage groups (LG), and covering 851 cM. Thanks to 84 common markers, we have stated that marker order was well conserved, except for LG I; significant unequal recombination rates occurred over the whole genome; regions with markers showing skewed segregation patterns differed between the two maps. Our results suggested a strong impact of the genetic background on recombination ability. Consequences for mushroom breeding are discussed. These maps will facilitate further comparative mapping studies of quantitative trait locus detection.

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Acknowledgments

The French Ministry of Agriculture is acknowledged for financial support through a CASDAR grant (project n° 2005-310). This research was also supported by INRA and CTC under joint contract.

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Author notes

  1. Anne Rodier

    Present address: Sylvan/Somycel, BP 25, Z.I. Sud, route de Tours, 37130, Langeais, France

Authors and Affiliations

  1. UR1264, Mycologie et Sécurité des Aliments, INRA, Centre de Recherche Bordeaux-Aquitaine, BP 81, 33883, Villenave d’Ornon Cedex, France

    Marie Foulongne-Oriol, Rémi Dufourcq, Cathy Spataro & Jean-Michel Savoie

  2. Centre Technique du Champignon, 22 rue Bizard, Munet, 49400, Distré, France

    Christine Devesse, Aurélien Broly & Anne Rodier

Authors
  1. Marie Foulongne-Oriol
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  2. Rémi Dufourcq
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  4. Christine Devesse
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Correspondence to Marie Foulongne-Oriol.

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Communicated by U. Kues.

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Foulongne-Oriol, M., Dufourcq, R., Spataro, C. et al. Comparative linkage mapping in the white button mushroom Agaricus bisporus provides foundation for breeding management. Curr Genet 57, 39–50 (2011). https://doi.org/10.1007/s00294-010-0325-z

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