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Plant Growth-Promoting Attributes of Zinc Solubilizing Dietzia maris Isolated from Polyhouse Rhizospheric Soil of Punjab

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Abstract

Zinc solubilizing rhizobacteria (ZSR) enhance the phyto-availability of Zn by converting its insoluble forms into usable forms that are essential for the growth and nutritional quality of crops. In the present study, a potential ZSR, hereafter referred to as strain N14, was isolated from the polyhouse rhizospheric soil of Punjab, India. The isolated rhizobacteria was found to be Gram-positive, aerobic, rod-shaped, and demonstrated a solubilization index of 63.75 on the Bunt Rovira (BR) medium. The 16S rRNA gene sequence analysis revealed that isolated strain N14 matches substantially with type strain Dietzia maris DSM 43672 T. In its ZnO broth assay, a significant amount of soluble Zn was detected along with a simultaneous decrease in pH of the broth. Ultra-performance liquid chromatography analysis revealed the release of organic acids, specifically, lactic acid and acetic acid by D. maris strain N14 which could be the reason for the decrease in broth pH. The production of indole acetic acid (29.91 µg/ml), gibberellic acid (4.72 µg/ml), ammonia (38.87 µg/ml), siderophore (0.89%), along with the release of HCN and appearance of phosphate solubilization zone (14.4 mm) with this strain suggested its possible plant growth-promoting (PGP) characteristics. Therefore, this strain was employed in the formulation of pellets which were applied for in vivo PGP studies using tomato plants. The developed bioformulated pellets showed a significant enhancement in plant growth as compared to control and vermicompost treated plants. To the best of our knowledge, this is the first report describing the Zn solubilizing and PGP characteristics of D. maris.

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Data Availability

The sequencing data of Dietzia maris strain N14 was deposited in NCBI with GenBank accession no. MZ191749.

Code Availability

Not applicable.

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Acknowledgements

All authors acknowledge the support of the Center of Innovative and Applied Bioprocessing, Mohali, India for providing access to Ultra-performance liquid chromatography (UPLC). All the authors are thankful to Chandigarh University for providing financial support, lab, and other infrastructure.

Funding

The study was carried out using the internal funds of Chandigarh University.

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Authors and Affiliations

  1. University Institute of Biotechnology, Chandigarh University, Mohali, 140413, Punjab, India

    Nitu Rani & Sukhminderjit Kaur

  2. University Institute of Agricultural Sciences, Chandigarh University, Mohali, 140413, Punjab, India

    Gurparteek Kaur

  3. Department of Chemistry, University Institute of Science, Chandigarh University, Mohali, 140413, Punjab, India

    Vishal Mutreja

  4. Centre of Innovative and Applied Bioprocessing, Mohali, 140413, Punjab, India

    Nishant Pandey

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  1. Nitu Rani
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Contributions

The research proposal was designed by SK. NR performed the experimental work. Data collection, analysis, and paper writing were done by NR and GK. The UPLC analysis and its interpretation was done by NP. VM contributed in preparing high-resolution figures and the final editing of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Sukhminderjit Kaur.

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Rani, N., Kaur, G., Kaur, S. et al. Plant Growth-Promoting Attributes of Zinc Solubilizing Dietzia maris Isolated from Polyhouse Rhizospheric Soil of Punjab. Curr Microbiol 80, 48 (2023). https://doi.org/10.1007/s00284-022-03147-2

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