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Plant growth-promoting potential of ‘Myroides gitamensis’ isolated from virgin soils of Punjab

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Abstract

Phosphate-solubilizing (PS) and phosphate-mineralizing (PM) bacteria are considered vital for augmenting the plant growth through phosphorus mobilization and plant growth-promoting attributes. In the present study, a rhizospheric bacterium was isolated from the virgin land of Punjab, India and identified as ‘Myroides gitamensis’ BSH-3 through 16S rRNA sequencing. ‘M. gitamensis’ showed potential halo zone on Pikovskaya agar. The novelty of the study lies in the fact that plant growth-promoting potential of ‘M. gitamensis’ has not been studied earlier. It was able to solubilize 17.53–106.66 µg/mL of tricalcium phosphate and demonstrated a promising potential of mineralizing sodium phytate corresponding to 44.6–94.70 µg/mL at 28 °C. Variable PS and PM activity was observed at temperature range of 15–42 °C with the maximum activity observed at 28 °C after 96 h of incubation. The nitrogen fixation ability, hydrogen sulfide production, cellulose hydrolysis test and chitin degradation was found to be negative. High indole acetic acid (42.82 µg/mL), gibberellic acid (72.93 µg/mL), ammonia (22.58 µg/mL) production, phytase activity (0.49 pi/mL/min) and comparable amount of siderophore (28.55%) and acid phosphate activity (0.606 µM p-nitrophenol/ml/min) was shown by ‘M. gitamensis’. Inoculation of wheat with ‘M. gitamensis’ in pot experiment showed increased shoot and root length by 30.58% and 38.32%. Fresh weight and dry weight was increased by 45.74% and 67.81%, respectively, compared to uninoculated control. These results demonstrate that ‘M. gitamensis’ has promising PS, PM and plant growth-promoting attributes to be used as a bio-inoculant to enhance plant growth and soil fertility.

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Funding

The authors are grateful to the University Grants Commission, New Delhi, India, for awarding the financial assistance to Rajinder Kaur, in the form of Junior Research Fellow under the scheme National Fellowship for OBC students.

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Authors and Affiliations

  1. Department of Biotechnology, Chandigarh University, Gharaun, Mohali, Punjab, 140413, India

    Rajinder Kaur & Sukhminderjit Kaur

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  1. Rajinder Kaur
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The research proposal was designed by Dr. SK and Ms. RK performed the experimental work and paper writing. Final paper editing and improvement was followed by Dr. SK.

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Correspondence to Sukhminderjit Kaur.

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The authors declare that they have no financial or non-financial conflict of interest.

Ethics statement

The biosafety test of the bacterial isolate was performed using blood agar. For preparing blood agar, self-blood (2.5 mL) was used with proper care in the presence of a medical practitioner. All the procedures undertaken were in accordance with the ethical standards approved by the Departmental Research Committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Communicated by Erko Stackebrandt.

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Kaur, R., Kaur, S. Plant growth-promoting potential of ‘Myroides gitamensis’ isolated from virgin soils of Punjab. Arch Microbiol 203, 2551–2561 (2021). https://doi.org/10.1007/s00203-021-02231-8

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  • DOI: https://doi.org/10.1007/s00203-021-02231-8

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