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The effect of gamma radiation on the lipid profile of irradiated red blood cells

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Abstract

An investigation into the effects of irradiation and of the storage time on aging and quality are a relevant issue to ensure the safety and the efficiency of irradiation in the prevention of transfusion-associated graft-versus-host disease (TA-GVHD). In this work, the biochemical properties and alterations presented by erythrocyte membranes, up to 28-days post-irradiation, with a dose of 25 Gy, were studied as a function of storage and post-irradiation time. There was a considerable variation in the total of phospholipid content, when comparing the control and irradiated samples, mostly from the third day onwards; and at the same time, the effect occurred as a function on the storage time of blood bags. The levels of total cholesterol decreased 3–9 days after irradiation. TBARS levels were increased after irradiation and 7 days of storage, but no increment of catalase activity was observed after the irradiation. Furthermore, the protein profile was maintained throughout the irradiation and storage time, until the 21st day, with the presence of a protein fragmentation band of around 28 kDa on the 28th day. In conclusion, although gamma irradiation is the main agent for the prevention of TA-GVHD, a better understanding of the physical and biochemical properties of erythrocytes are necessary to better assess their viability, and to be able to issue more secure recommendations on the shelf life of blood bags, and the safe use of the irradiated red cells therein.

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Acknowledgments

The authors are grateful to Fundação HemoMinas – Divinópolis for red blood cell supply. The work is supported by FAPEMIG (Fundação de Amparo a Pesquisa do Estado de Minas Gerais) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico). The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Faculdade de Bioquímica, Programa de Pós-Graduação em Ciências da Saúde, Universidade Federal de São João del Rei, Campus Centro-Oeste Dona Lindú, Av Sebastião Gonçalves Coelho, 400, Bairro Chanadour, CEP, 35501-296, Divinópolis, Minas Gerais, Brazil

    Grazielle Aparecida Silva Maia, Cristiane de Oliveira Renó, Vanessa Faria Cortes, Leandro Augusto Barbosa & Hérica de Lima Santos

  2. Laboratório de Bioquímica de Lipídeos e Lipoproteínas, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

    Jorge Mansur Medina, Alan Barbosa da Silveira & Georgia Correa Atella

  3. Laboratório de Estrutura e Regulação de ATPases, Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

    Julio Alberto Mignaco

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  1. Grazielle Aparecida Silva Maia
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  2. Cristiane de Oliveira Renó
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  7. Vanessa Faria Cortes
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  8. Leandro Augusto Barbosa
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  9. Hérica de Lima Santos
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Correspondence to Leandro Augusto Barbosa or Hérica de Lima Santos.

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Maia, G.A.S., de Oliveira Renó, C., Medina, J.M. et al. The effect of gamma radiation on the lipid profile of irradiated red blood cells. Ann Hematol 93, 753–760 (2014). https://doi.org/10.1007/s00277-013-1944-5

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  • DOI: https://doi.org/10.1007/s00277-013-1944-5

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