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Relationship between (Na + K)-ATPase activity, lipid peroxidation and fatty acid profile in erythrocytes of hypertensive and normotensive subjects

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Abstract

Oxidative stress may play a role in the pathogenic mechanism of essential hypertension. Lipid peroxidation can alter the cellular structure of membrane-bound enzymes by changing the membrane phospholipids fatty acids composition. We investigated the relationship between (Na + K)-ATPase activity, lipid peroxidation, and erythrocyte fatty acid composition in essential hypertension. The study included 40 essential hypertensive and 49 healthy normotensive men (ages 35–60 years). Exclusion criteria were obesity, dyslipidemia, diabetes mellitus, smoking, and any current medication. Patients underwent 24-h ambulatory blood pressure monitoring and blood sampling. Lipid peroxidation was measured in the plasma and erythrocytes as 8-isoprostane or malondialdehyde (MDA), respectively. Antioxidant capacity was measured as ferric reducing ability of plasma (FRAP) in the plasma and as reduced/oxidized glutathione (GSH/GSSG ratio) in erythrocytes. (Na + K)-ATPase activity and fatty acids were determined in erythrocyte membranes. Hypertensives had higher levels of plasma 8-isoprostane, erythrocyte MDA, and relative percentage of saturated membrane fatty acids, but lower plasma FRAP levels, erythrocyte GSH/GSSG ratio, (Na + K)-ATPase activity and relative percentage of unsaturated membrane fatty acids, compared with normotensives. Day-time systolic and diastolic blood pressures correlated positively with lipid peroxidation parameters, but negatively with (Na + K)-ATPase activity. These findings suggest that the modulation of (Na + K)-ATPase activity may be associated with changes in the fatty acid composition induced by oxidative stress and provide evidence of a role for this enzyme in the pathophysiology of essential hypertension.

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Acknowledgments

The authors wish to thank the Fondo Nacional de Investigación Científica y Tecnológica (FONDECYT, grant number 1040429, Chile Government), Procaps Laboratory (Colombia) and Gynopharm CFR Laboratory (Chile) for their financial support of this study. The technical assistance of Mr. Diego Soto is also acknowledged.

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Authors and Affiliations

  1. Laboratory of Renal Pathophysiology, Molecular and Clinical Pharmacology Program, Institute of Biomedical Sciences, Faculty of Medicine, University of Chile, Independencia 1027, Casilla, 70058, Santiago 7, Chile

    Ramón Rodrigo & Jean P. Bächler

  2. Department of Nutrition, Faculty Of Medicine, University of Chile, Santiago, Chile

    Julia Araya

  3. Cardiovascular Center, University of Chile Clinical Hospital, Santiago, Chile

    Hernán Prat

  4. Nephrology Unit, University of Chile Clinical Hospital, Santiago, Chile

    Walter Passalacqua

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  1. Ramón Rodrigo
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  2. Jean P. Bächler
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  5. Walter Passalacqua
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Correspondence to Ramón Rodrigo.

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Rodrigo, R., Bächler, J.P., Araya, J. et al. Relationship between (Na + K)-ATPase activity, lipid peroxidation and fatty acid profile in erythrocytes of hypertensive and normotensive subjects. Mol Cell Biochem 303, 73–81 (2007). https://doi.org/10.1007/s11010-007-9457-y

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