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Single Mechanosensitive and Ca2+-Sensitive Channel Currents Recorded from Mouse and Human Embryonic Stem Cells

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Abstract

Cell-attached and inside-out patch clamp recording was used to compare the functional expression of membrane ion channels in mouse and human embryonic stem cells (ESCs). Both ESCs express mechanosensitive Ca2+ permeant cation channels (MscCa) and large conductance (200 pS) Ca2+-sensitive K+ (BKCa2+) channels but with markedly different patch densities. MscCa is expressed at higher density in mESCs compared with hESCs (70 % vs. 3 % of patches), whereas the BKCa2+ channel is more highly expressed in hESCs compared with mESCs (~50 % vs. 1 % of patches). ESCs of both species express a smaller conductance (25 pS) nonselective cation channel that is activated upon inside-out patch formation but is neither mechanosensitive nor strictly Ca2+-dependent. The finding that mouse and human ESCs express different channels that sense membrane tension and intracellular [Ca2+] may contribute to their different patterns of growth and differentiation in response to mechanical and chemical cues.

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Acknowledgments

OH was supported by a travel/stay Grant from Ministerio de Educación y Ciencia (SAB2006-0211) and in the United States by grants from the National Cancer Institute and the Department of Defense. BS and AH are supported by the Fundación Progreso y Salud, Consejería de Salud, Junta de Andalucía (PI-0022/2008); Consejería de Innovación Ciencia y Empresa, Junta de Andalucía (CTS-6505; INP-2011-1615-900000); FEDER cofunded grants from Instituto de Salud Carlos III (Red TerCel-RD06/0010/0025; PI10/00964), and the Ministry of Health and Consumer Affairs (Advanced Therapies Program TRA-120). CIBERDEM is an initiative of the Instituto de Salud Carlos III.

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  1. Department of Stem Cells, Andalusian Center for Molecular Biology and Regenerative Medicine (CABIMER), Seville, Spain

    Bernat Soria, Sergio Navas & Abdelkrim Hmadcha

  2. CIBER de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Barcelona, Spain

    Bernat Soria & Abdelkrim Hmadcha

  3. Department of Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, USA

    Owen P. Hamill

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  1. Bernat Soria
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Correspondence to Owen P. Hamill.

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Soria, B., Navas, S., Hmadcha, A. et al. Single Mechanosensitive and Ca2+-Sensitive Channel Currents Recorded from Mouse and Human Embryonic Stem Cells. J Membrane Biol 246, 215–230 (2013). https://doi.org/10.1007/s00232-012-9523-6

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