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Whole Cell Patch Clamp Electrophysiology in Human Neuronal Cells

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Stem Cell-Based Neural Model Systems for Brain Disorders

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2683))

Abstract

Whole cell patch clamp recording techniques are commonly used to assay membrane excitability, ion channel function, and synaptic activity in neurons. However, assaying these functional properties of human neurons remains difficult because of the difficulty in obtaining human neuronal cells. Recent advents in stem cell biology, especially the development of the induced pluripotent stem cells, made it possible to generate human neuronal cells in both 2-dimensional (2D) monolayer cultures and 3D brain-organoid cultures. Here, we describe the whole cell patch clamp methods of recording neuronal physiology from human neuronal cells.

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Acknowledgments

Pang lab is supported by NIAAA R01AA023797 and NIMH R21MH126420. A.J.B. was supported by NIGMS T32GM008339 and by NCATS TL1TR003019. The Child Health Institute of New Jersey is supported in part by the Robert Wood Johnson Foundation (grant #74260).

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Authors and Affiliations

  1. Child Health Institute of New Jersey, Robert Wood Johnson Medical School, New Brunswick, NJ, USA

    Rafael Gabriel III, Andrew J. Boreland & Zhiping P. Pang

  2. Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Piscataway, NJ, USA

    Andrew J. Boreland & Zhiping P. Pang

Authors
  1. Rafael Gabriel III
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  2. Andrew J. Boreland
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  3. Zhiping P. Pang
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Correspondence to Zhiping P. Pang .

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Editors and Affiliations

  1. Department of Molecular Biology, Cell Biology, and Biochemistry, Center for Translational Neuroscience, Carney Institute for Brain Science and Brown Institute of Translational Science, Brown University, Providence, RI, USA

    Yu-Wen Alvin Huang

  2. Department of Biochemistry and Molecular Biology, University of Massachusetts Amherst, Amherst, MA, USA

    ChangHui Pak

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Gabriel, R., Boreland, A.J., Pang, Z.P. (2023). Whole Cell Patch Clamp Electrophysiology in Human Neuronal Cells. In: Huang, YW.A., Pak, C. (eds) Stem Cell-Based Neural Model Systems for Brain Disorders. Methods in Molecular Biology, vol 2683. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3287-1_21

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  • DOI: https://doi.org/10.1007/978-1-0716-3287-1_21

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3286-4

  • Online ISBN: 978-1-0716-3287-1

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