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Novel synthesis of a dual fluorimetric sensor for the simultaneous analysis of levodopa and pyridoxine

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Abstract

Herein, a fluorimetric sensor was fabricated based on molecularly imprinted polymers (MIPs) with two types of carbon dots as fluorophores. The MIPs produced had similar excitation wavelengths (400 nm) and different emission wavelengths (445 and 545 nm). They were used for the simultaneous analysis of levodopa and pyridoxine. First, two types of carbon dots, i.e. nitrogen-doped carbon dots (NCDs) with a quantum yield of 43%, and carbon dots from o-phenylenediamine (O-CDs) with a quantum yield of 17%, were prepared using the hydrothermal method. Their surfaces were then covered with MIPs through the reverse microemulsion method. Finally, a mixture of powdered NCD@MIP and O-CD@MIP nanocomposites was used for the simultaneous fluorescence measurement of levodopa and pyridoxine. Under optimal conditions using response surface methodology and Design-Expert software, a linear dynamic range of 38 to 369 nM and 53 to 457 nM, and detection limits of 13 nM and 25 nM were obtained for levodopa and pyridoxine, respectively. The capability of the proposed fluorimetric sensor was investigated in human blood serum and urine samples.

Schematic representation of nitrogen-doped carbon dots (NCDs), carbon dots from o-phenylenediamine (O-CDs), NCDs coated with imprinted polymers (NCD@MIPs), and O-CDs coated with imprinted polymers (O-CD@MIPs) in the presence and absence of levodopa and pyridoxine.

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Acknowledgements

We wish to thank Isfahan University of Technology (IUT) Research Council and Center of Excellence in Sensor and Green Chemistry for their patronage and help in this study.

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  1. Department of Chemistry, Isfahan University of Technology, Emam Boulevard, Isfahan, 84156-83111, Iran

    Seyed Mohammad Ghani, Behzad Rezaei, Hamid Reza Jamei & Ali Asghar Ensafi

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  1. Seyed Mohammad Ghani
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  2. Behzad Rezaei
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Correspondence to Behzad Rezaei.

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All procedures performed in studies involving human samples were in accordance with the ethical standards of the institutional research committee of Isfahan University of Technology and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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The management of the Health Center of Isfahan University of Technology has authorized the use of blood and urine samples in this study, and the publication of the results of the study of the mentioned samples has no conflict of interest.

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Ghani, S.M., Rezaei, B., Jamei, H.R. et al. Novel synthesis of a dual fluorimetric sensor for the simultaneous analysis of levodopa and pyridoxine. Anal Bioanal Chem 413, 377–387 (2021). https://doi.org/10.1007/s00216-020-03005-9

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