Abstract
The authors describe a rapid enzyme-free colorimetric assay for food- and water-borne pathogens by using the multi-functional MnO2-doped magnetic ferrite nanoparticles (MCDs-MnO2 NPs). These nanoparticles not only can recognize, absorb, and separate the analyte from the matrix efficiently but also can directly catalyze the oxidation of TMB into a blue colored product without H2O2. In the presence of target, the nanoparticles preferentially bind to the target surface and the interaction between nanoparticles and TMB can be blocked, providing turn-off sensing of bacteria. By combining the superior capture efficiency of magnetic beads with the high catalytic activity of the enzyme mimic, the turn-off colorimetric assay can detect the gram-positive bacterium and the gram-negative bacterium by the naked eye at a low detection limit (102 cfu mL−1) with a wide line arrange from 10 to 106 cfu mL−1. With the advantage of simplicity, sensitivity, and specificity, this proposed approach showed promise in rapid instrumental and on-site visual detection of bacteria for determination of water contamination.
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Funding
This study received financial support from the National Natural Science Foundation of China (Grant Nos. 81602895, 81602894, and 81872668), the Education Department of Jilin Province (JJKH20180240KJ), Health Commission of Jilin Province (2018Q033), and Norman Bethune Health Science Center of Jilin University (2018A05).
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Liu, Y., Zhao, C., Zhao, W. et al. Multi-functional MnO2-doped Fe3O4 nanoparticles as an artificial enzyme for the colorimetric detection of bacteria. Anal Bioanal Chem 412, 3135–3140 (2020). https://doi.org/10.1007/s00216-020-02563-2
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DOI: https://doi.org/10.1007/s00216-020-02563-2