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Ionic liquids functionalized Fe3O4-based colorimetric biosensor for rapid determination of ochratoxin A

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Abstract

A stainless steel mesh (SSM) with the feature of flexibility was employed as the colorimetric biosensor substrate, and aptamer was bond onto the surface of the SSM. Through the cross-linking of ionic liquids (ILs), AuPt nanoparticles were deposited  onto the surface of Fe3O4 material to obtain a magnetic nanozyme with high peroxidase catalytic activity and rapid color change. Through the competing interaction of OTA and cDNA with aptamer, AuPt@IL@Fe3O4 signal probe was separated to catalyze the 3,3′,5,5′-tetramethylbenzidine/hydrogen peroxide (TMB/H2O2) system to observe the color by bare eye and record the absorbance at 652 nm using a UV-spectrophotometer. Through the study of the catalytic properties on the basis of the Michaelis equation, AuPt@IL@Fe3O4 nanozyme presented a Vmax of 3.85 × 10−8 M s−1 and Km of 0.01 mM. Under the optimized conditions, the linear range of the colorimetric biosensor towards OTA was 5–100 ng mL−1, and the detection limit was 0.078 ng mL–1. This biosensor was applied to beer and corn samples with recoveries of 70.4–102.6% and 93.3–104.7%, respectively. Results showed that this sensor is a portable, rapid, economical, sensitive visual sensing platform towards mycotoxin in real samples.

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Funding

This work was supported by the National Natural Science Foundation of China (No. 31901766), financial support from the Talents of High-Level Scientific Research Foundation, Qingdao Agricultural University (No.1119014), postgraduate innovation gram of Qingdao Agricultural University (No. QNYCX22096), Rural Revitalization Science and Technology Innovation Plan Project of Shandong Province (2022TZXD0031), and Science & Technology Specific Projects in Agricultural High-tech Industrial Demonstration Area of the Yellow River Delta (2022SZX26).

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Authors and Affiliations

  1. College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China

    Qianwen Liu, Siyu Xin, Xin Tan, Qingli Yang & Xiudan Hou

  2. Academy of Dongying Efficient Agricultural Technology and Industry on Saline and Alkaline Land in Collaboration with Qingdao Agricultural University, Dongying, 257343, China

    Qianwen Liu & Xiudan Hou

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Correspondence to Xiudan Hou.

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Liu, Q., Xin, S., Tan, X. et al. Ionic liquids functionalized Fe3O4-based colorimetric biosensor for rapid determination of ochratoxin A. Microchim Acta 190, 364 (2023). https://doi.org/10.1007/s00604-023-05943-4

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