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Direct determination of phosphate sugars in biological material by 1H high-resolution magic-angle-spinning NMR spectroscopy

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Abstract

The study aim was to unambiguously assign nucleotide sugars, mainly UDP-X that are known to be important in glycosylation processes as sugar donors, and glucose-phosphates that are important intermediate metabolites for storage and transfer of energy directly in spectra of intact cells, as well as in skeletal muscle biopsies by 1H high-resolution magic-angle-spinning (HR-MAS) NMR. The results demonstrate that sugar phosphates can be determined quickly and non-destructively in cells and biopsies by HR-MAS, which may prove valuable considering the importance of phosphate sugars in cell metabolism for nucleic acid synthesis. As proof of principle, an example of phosphate-sugar reaction and degradation kinetics after unfreezing the sample is shown for a cardiac muscle, suggesting the possibility to follow by HR-MAS NMR some metabolic pathways.

Glucose-phosphate sugars (Glc-1P and Glc-6P) detected in muscle by 1H HR-MAS NMR

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Authors and Affiliations

  1. Departments of Radiology and Clinical Research, University of Bern, Murtenstrasse 35, 3008, Bern, Switzerland

    Gaëlle Diserens, Chris Boesch & Peter Vermathen

  2. Graduate School for Cellular and Biomedical Sciences, University of Bern, Freiestrasse 1, 3012, Bern, Switzerland

    Gaëlle Diserens

  3. Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, 3012, Bern, Switzerland

    Martina Vermathen & Ilche Gjuroski

  4. University Institute of Clinical Chemistry, Bern University Hospital, F/INO, 3010, Bern, Switzerland

    Sandra Eggimann

  5. Department of Clinical Veterinary Medicine, University of Bern, Länggassstrasse 124, 3001, Bern, Switzerland

    Christina Precht

Authors
  1. Gaëlle Diserens
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  2. Martina Vermathen
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  3. Ilche Gjuroski
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  4. Sandra Eggimann
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  5. Christina Precht
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  6. Chris Boesch
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  7. Peter Vermathen
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Correspondence to Gaëlle Diserens.

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The authors declare that they have no conflict of interest.

Ethical approval

Ethical approval and informed consent of all patients were obtained for getting biopsies.

Funding

Financial support from the Swiss National Science Foundation SNF grants # 206021-128736(MV, PV), # 200021_149438(MV), and # 320030-138150(PV) is greatly acknowledged. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Diserens, G., Vermathen, M., Gjuroski, I. et al. Direct determination of phosphate sugars in biological material by 1H high-resolution magic-angle-spinning NMR spectroscopy. Anal Bioanal Chem 408, 5651–5656 (2016). https://doi.org/10.1007/s00216-016-9671-0

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  • DOI: https://doi.org/10.1007/s00216-016-9671-0

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