Abstract
Mulberry bacterial wilt disease, caused by Ralstonia pseudosolanacearum, is a devastating soil-borne disease in the silk-mulberry-related industry. In this study, through high-throughput sequencing, we compared the rhizosphere bacterial composition of the mulberry-resistant cultivar (K10) and susceptible cultivar (G12), confirming Bacillus as a genus-level biomarker for K10. Next, twelve Bacillus spp. isolates, derived from the rhizosphere of K10, were screened for their antagonistic activity against R. pseudosolanacearum. The isolate showing strong antagonism was identified as B. velezensis K0T24 and selected for further analysis. The fermentation supernatant of B. velezensis K0T24 significantly inhibited the growth of R. pseudosolanacearum (82.47%) and the expression of its pathogenic genes. Using B. velezensis K0T24 in mulberry seedlings also increased defense enzyme activities and achieved a control efficacy of up to 55.17% against mulberry bacterial wilt disease. Collectively, our findings demonstrate the potential of B. velezensis K0T24 in suppressing mulberry bacterial wilt disease.
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Acknowledgements
We thank Prof. Wei Ding for providing us with the greenhouse facility and Prof. Tongbao Liu for his critical reading of the manuscript and helpful suggestions. We also thank for the lab classmates Yupan Qu, Keyao Zhang, Yan Li and Wenqi Yang for assisting in some experiments.
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This work was financially supported by the National Natural Science Foundation of China (32371713 and 32102300), the Fundamental Research Funds for the Central Universities (SWU-KQ22082) and the Natural Science Foundation of Chongqing Municipality (CSTB2022NSCQ-MSX0536).
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Wenlian Jiao: Conceptualization, Investigation, Data curation, Writing -original draft, Formal analysis, Visualization. Ju Wen: Investigation, Writing -original draft, Formal analysis, Visualization. Na Li: Investigation, Formal analysis. Ting Ou: Methodology, Visualization. Changyu Qiu: Resources. Yutong Ji: Investigation, Formal analysis. Kai Lin: Investigation, Formal analysis. Xiaojiao Liu: Conceptualization, Methodology, Project administration, Funding acquisition, Supervision, Writing - review & editing, Visualization. Jie Xie: Conceptualization, Project administration, Funding acquisition, Resources, Supervision, Writing - review & editing.
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Jiao, W., Wen, J., Li, N. et al. The biocontrol potentials of rhizospheric bacterium Bacillus velezensis K0T24 against mulberry bacterial wilt disease. Arch Microbiol 206, 213 (2024). https://doi.org/10.1007/s00203-024-03935-3
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DOI: https://doi.org/10.1007/s00203-024-03935-3