Abstract
This study aimed to reveal that the effect of biosurfactant on the dispersion and degradation of crude oil. Whole genome analysis showed that Pseudomonas aeruginosa GB-3 contained abundant genes involved in biosurfactant synthesis and metabolic processes and had the potential to degrade oil. The biosurfactant produced by strain GB-3 was screened by various methods. The results showed that the surface tension reduction activity was 28.6 mN·m−1 and emulsification stability was exhibited at different pH, salinity and temperature. The biosurfactant was identified as rhamnolipid by LC–MS and FTIR. The fermentation conditions of strain GB-3 were optimized by response surface methodology, finally the optimal system (carbon source: glucose, nitrogen source: ammonium sulfate, C/N ratio:16:1, pH: 7, temperature: 30–35 °C) was determined. Compared with the initial fermentation, the yield of biosurfactant increased by 4.4 times after optimization. In addition, rhamnolipid biosurfactant as a dispersant could make the dispersion of crude oil reach 38% within seven days, which enhanced the bioavailability of crude oil. As a biostimulant, it could also improve the activity of indigenous microorganism and increase the degradation rate of crude oil by 10–15%. This study suggested that rhamnolipid biosurfactant had application prospect in bioremediation of marine oil-spill.
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References
Abdel-Mawgoud AM, Aboulwafa MM, Hassouna NA (2009) Characterization of rhamnolipid produced by Pseudomonas aeruginosa isolate Bs20. Appl Biochem Biotechnol 157(2):329–345. https://doi.org/10.1007/s12010-008-8285-1
Acosta-González A, Marqués S (2016) Bacterial diversity in oil-polluted marine coastal sediments. Curr Opin Biotechnol 38:24–32. https://doi.org/10.1016/j.copbio.2015.12.010
Ahmad Z, Zhang X, Imran M, Zhong H, Andleeb S, Zulekha R, Liu G, Ahmad I, Coulon F (2021) Production, functional stability, and effect of rhamnolipid biosurfactant from Klebsiella sp on phenanthrene degradation in various medium systems. Ecotox Environ Saf. https://doi.org/10.1016/j.ecoenv.2020.111514
Arcus VL, van der Kamp MW, Pudney CR, Mulholland AJ (2020) Enzyme evolution and the temperature dependence of enzyme catalysis. Curr Opin Struct Biol 65:96–101. https://doi.org/10.1016/j.sbi.2020.06.001
Arora P, Kshirsagar PR, Rana DP, Dhakephalkar PK (2019) Hyperthermophilic Clostridium sp. N-4 produced a glycoprotein biosurfactant that enhanced recovery of residual oil at 96 degrees C in lab studies. Colloids Surf B Biointerfaces. https://doi.org/10.1016/j.colsurfb.2019.110372
Arora PK, Nayarisseri A, Singh SK (2023) Genome analysis of biosurfactant producing bacterium. Plos One, Bacillus tequilensis. https://doi.org/10.1371/journal.pone.0285994
Atakpa EO, Zhou H, Jiang L, Zhang D, Li Y, Zhang W, Zhang C (2023) Co-culture of Acinetobacter sp and Scedosporium sp immobilized beads for optimized biosurfactant production and degradation of crude oil. Environ Pollut. https://doi.org/10.1016/j.envpol.2023.122365
Atlas RM, Hazen TC (2011) Oil biodegradation and bioremediation: a tale of the two worst spills in US history. Environ Sci Technol 45(16):6709–6715. https://doi.org/10.1021/es2013227
Ayirala S, Sofi A, Li Z, Xu Z (2021) Surfactant and surfactant-polymer effects on wettability and crude oil liberation in carbonates. J Petrol Sci Eng. https://doi.org/10.1016/j.petrol.2021.109117
Baharuddin SH, Mustahil NA, Reddy AVB, Abdullah AA, Mutalib MIA, Moniruzzaman M (2020) Development, formulation and optimization of a novel biocompatible ionic liquids dispersant for the effective oil spill remediation. Chemosphere. https://doi.org/10.1016/j.chemosphere.2020.126125
Bakhshi N, Soleimanian-Zad S, Sheikh-Zeinoddin M (2017) Dynamic surface tension measurement for the screening of biosurfactants produced by Lactobacillus plantarum subsp. plantarum PTCC 1896. Enzyme Microb Technol 101:1–8. https://doi.org/10.1016/j.enzmictec.2017.02.010
Barakat KM, Hassan SWM, Darwesh OM (2017) Biosurfactant production by haloalkaliphilic Bacillus strains isolated from Red Sea Egypt. Egypt J Aquat Res 43(3):205–211. https://doi.org/10.1016/j.ejar.2017.09.001
Beyer J, Trannum HC, Bakke T, Hodson PV, Collier TK (2016) Environmental effects of the Deepwater Horizon oil spill: A review. Mar Pollut Bull 110(1):28–51. https://doi.org/10.1016/j.marpolbul.2016.06.027
Bose S, Senthil Kumar P, Rangasamy G (2024) Exploring the role of biosurfactants in the remediation of organic contaminants with a focus on the mechanism- a review. J Mol Liq. https://doi.org/10.1016/j.molliq.2023.123585
Brussaard CP, Peperzak L, Beggah S, Wick LY, Wuerz B, Weber J, Samuel Arey J, van der Burg B, Jonas A, Huisman J, van der Meer JR (2016) Immediate ecotoxicological effects of short-lived oil spills on marine biota. Nat Commun 7:11206. https://doi.org/10.1038/ncomms11206
Cai Q, Zhu Z, Chen B, Lee K, Nedwed TJ, Greer C, Zhang B (2021) A cross-comparison of biosurfactants as marine oil spill dispersants: Governing factors, synergetic effects and fates. J Hazard Mater. https://doi.org/10.1016/j.jhazmat.2021.126122
Cameotra SS, Singh P (2009) Synthesis of rhamnolipid biosurfactant and mode of hexadecane uptake by Pseudomonas species. Microb Cell Fact 8:16. https://doi.org/10.1186/1475-2859-8-16
Chafale A, Kapley A (2022) Biosurfactants as microbial bioactive compounds in microbial enhanced oil recovery. J Biotechnol 352:1–15. https://doi.org/10.1016/j.jbiotec.2022.05.003
Charles Oluwaseun A, Julius Kola O, Mishra P, Ravinder Singh J, Kumar Singh A, Singh Cameotra S, Oluwasesan Micheal B (2017) Characterization and optimization of a rhamnolipid from Pseudomonas aeruginosa C1501 with novel biosurfactant activities. Sustain Chem Pharm 6:26–36. https://doi.org/10.1016/j.scp.2017.07.001
Chen H, Wang Z, Huang Y, Wei J, Guo G, Miao L (2023) Anaerobic co-metabolic degradation of ceftriaxone sodium: performance and mechanism. J Clean Product. https://doi.org/10.1016/j.jclepro.2023.136388
Christensen BE, Kjosbakken J, Smidsrød O (1985) Partial chemical and physical characterization of two extracellular polysaccharides produced by marine, periphytic Pseudomonas sp. strain NCMB 2021. Appl Environ Microbiol 50:837–845. https://doi.org/10.1128/aem.50.4.837-845.1985
Dai X, Lv J, Zhang Z, Wang H (2023) Bioremediation of heavy oil-contaminated intertidal zones using slow-release nutrients and rhamnolipid biosurfactants. J Environ Chem Eng. https://doi.org/10.1016/j.jece.2023.109323
Daneshgar Asl S, Dukhovskoy DS, Bourassa M, MacDonald IR (2017) Hindcast modeling of oil slick persistence from natural seeps. Remote Sens Environ 189:96–107. https://doi.org/10.1016/j.rse.2016.11.003
Das S, Das N, Choure K, Pandey P (2023) Biodegradation of asphaltene by lipopeptide-biosurfactant producing hydrocarbonoclastic, crude oil degrading Bacillus spp. Bioresour Technol. https://doi.org/10.1016/j.biortech.2023.129198
Dhaka A, Chattopadhyay P (2021) A review on physical remediation techniques for treatment of marine oil spills. J Environ Manage. https://doi.org/10.1016/j.jenvman.2021.112428
Durval IJB, Mendonca AHR, Rocha IV, Luna JM, Rufino RD, Converti A, Sarubbo LA (2020) Production, characterization, evaluation and toxicity assessment of a Bacillus cereus UCP 1615 biosurfactant for marine oil spills bioremediation. Mar Pollut Bull. https://doi.org/10.1016/j.marpolbul.2020.111357
Escobar H (2019) Mystery oil spill threatens marine sanctuary in Brazil. Science 366(6466):672. https://doi.org/10.1126/science.366.6466.672
Ganesh Kumar A, Manisha D, Nivedha Rajan N, Sujitha K, Magesh Peter D, Kirubagaran R, Dharani G (2023) Biodegradation of phenanthrene by piezotolerant Bacillus subtilis EB1 and genomic insights for bioremediation. Marine Pollut Bull. https://doi.org/10.1016/j.marpolbul.2023.115151
Gangola S, Bhatt P, Kumar AJ, Bhandari G, Joshi S, Punetha A, Bhatt K, Rene ER (2022) Biotechnological tools to elucidate the mechanism of pesticide degradation in the environment. Chemosphere. https://doi.org/10.1016/j.chemosphere.2022.133916
Gangola S, Bhandari G, Joshi S, Sharma A, Simsek H, Bhatt P (2023) Esterase and ALDH dehydrogenase-based pesticide degradation by Bacillus brevis 1B from a contaminated environment. Environ Res. https://doi.org/10.1016/j.envres.2023.116332
Gaur VK, Gupta P, Tripathi V, Thakur RS, Regar RK, Patel DK, Manickam N (2022) Valorization of agro-industrial waste for rhamnolipid production, its role in crude oil solubilization and resensitizing bacterial pathogens. Environ Technol Innovat. https://doi.org/10.1016/j.eti.2021.102108
Geng X, Barker CH, MacFadyen A, Boufadel MC, Lee K, Thrift-Viveros DL, Jones R, O’Connor C (2022) Oil biodegradation in permeable marine sediments: Effects of benthic pore-water advection and solute exchange. J Hazard Mater. https://doi.org/10.1016/j.jhazmat.2022.129211
Han W, Tan J, Peng L, Liu L, Zhou X, Zhang W, Shi B (2019) Ecotoxicity and micellization behavior of anionic surfactant sodium dodecylbenzene sulfonate (SDBS) and its mixtures with nonionic surfactant fatty alcohol-polyoxyethylene ether (AEO). Aquat Toxicol. https://doi.org/10.1016/j.aquatox.2019.105313
Jabeen H, Iqbal S, Anwar S, Parales RE (2015) Optimization of profenofos degradation by a novel bacterial consortium PBAC using response surface methodology. Int Biodeterior Biodegradation 100:89–97. https://doi.org/10.1016/j.ibiod.2015.02.022
Janek T, Lukaszewicz M, Krasowska A (2013) Identification and characterization of biosurfactants produced by the Arctic bacterium Pseudomonas putida BD2. Colloids Surf B Biointerfaces 110:379–386. https://doi.org/10.1016/j.colsurfb.2013.05.008
Jin J, Wang H, Jing Y, Liu M, Wang D, Li Y, Bao M (2019) An efficient and environmental-friendly dispersant based on the synergy of amphiphilic surfactants for oil spill remediation. Chemosphere 215:241–247. https://doi.org/10.1016/j.chemosphere.2018.09.159
Kalvandi S, Garousin H, Pourbabaee AA, Farahbakhsh M (2022) The release of petroleum hydrocarbons from a saline-sodic soil by the new biosurfactant-producing strain of Bacillus sp. Scie Rep. https://doi.org/10.1038/s41598-022-24321-3
Kim HS, Dong K, Kim J, Lee SS (2018) Characteristics of crude oil-degrading bacteria Gordonia iterans isolated from marine coastal in Taean sediment. MicrobiologyOpen. https://doi.org/10.1002/mbo3.754
Kleindienst S, Paul JH, Joye SB (2015) Using dispersants after oil spills: impacts on the composition and activity of microbial communities. Nat Rev Microbiol 13(6):388–396. https://doi.org/10.1038/nrmicro3452
Kovačević A, Marković D, Radoičić M, Šaponjić Z, Radetić M (2023) Sustainable non-woven sorbents based on jute post-industrial waste for cleaning of oil spills. J Clean Product. https://doi.org/10.1016/j.jclepro.2022.135811
Li Z, Zhang Y, Lin J, Wang W, Li S (2019) High-Yield Di-Rhamnolipid Production by Pseudomonas aeruginosa YM4 and its Potential Application in MEOR. Molecules. https://doi.org/10.3390/molecules24071433
Li Y, Yang G, Weng Y, Xu L, Hou F, Devkota S, Zhao C-X (2023) Exploring biosurfactants as a sustainable alternative to chemical surfactants. Coll Surf Physicochem Eng Aspects. https://doi.org/10.1016/j.colsurfa.2023.132291
Liu Y, Ma X, Zeng G, Zhong H, Liu Z, Jiang Y, Yuan X, He X, Lai M, He Y (2014) Role of low-concentration monorhamnolipid in cell surface hydrophobicity of Pseudomonas aeruginosa: adsorption or lipopolysaccharide content variation. Appl Microbiol Biotechnol 98(24):10231–10241. https://doi.org/10.1007/s00253-014-5957-3
Liu Y, Zeng G, Zhong H, Wang Z, Liu Z, Cheng M, Liu G, Yang X, Liu S (2017) Effect of rhamnolipid solubilization on hexadecane bioavailability: enhancement or reduction? J Hazard Mater 322(Pt B):394–401. https://doi.org/10.1016/j.jhazmat.2016.10.025
Liu S, Xu N, Liu H, Zhou J, Xin F, Zhang W, Qian X, Jiang M, Dong W (2020) Genome Characterization of Pseudomonas aeruginosa KT1115, a High Di-rhamnolipid-Producing Strain with Strong Oils Metabolizing Ability. Curr Microbiol 77(8):1890–1895. https://doi.org/10.1007/s00284-020-02009-z
Lotfabad TB, Abassi H, Ahmadkhaniha R, Roostaazad R, Masoomi F, Zahiri HS, Ahmadian G, Vali H, Noghabi KA (2010) Structural characterization of a rhamnolipid-type biosurfactant produced by Pseudomonas aeruginosa MR01: enhancement of di-rhamnolipid proportion using gamma irradiation. Colloids Surf B Biointerfaces 81(2):397–405. https://doi.org/10.1016/j.colsurfb.2010.06.026
Lu Y, Song S, Tian H, Yu H, Zhao J, Chen C (2018) Functional analysis of the role of CcpA in Lactobacillus plantarum grown on fructooligosaccharides or glucose: a transcriptomic perspective. Microb Cell Factor. https://doi.org/10.1186/s12934-018-1050-4dvyh6gf
Mapelli F, Scoma A, Michoud G, Aulenta F, Boon N, Borin S, Kalogerakis N, Daffonchio D (2017) Biotechnologies for Marine Oil Spill Cleanup: Indissoluble Ties with Microorganisms. Trends Biotechnol 35(9):860–870. https://doi.org/10.1016/j.tibtech.2017.04.003
Maulana A, Adiandri RS, Boopathy R, Setiadi T (2022) Extraction, characterization, and biosurfactant properties of extracellular polymeric substance from textile wastewater activated sludge. J Biosci Bioeng 134(6):508–512. https://doi.org/10.1016/j.jbiosc.2022.08.005
Nagtode VS, Cardoza C, Yasin HKA, Mali SN, Tambe SM, Roy P, Singh K, Goel A, Amin PD, Thorat BR, Cruz JN, Pratap AP (2023) Green Surfactants (Biosurfactants): A Petroleum-Free Substitute for Sustainability─Comparison, Applications, Market, and Future Prospects. ACS Omega 8(13):11674–11699. https://doi.org/10.1021/acsomega.3c00591
Nikolova CN, Ijaz UZ, Magill C, Kleindienst S, Joye SB, Gutierrez T (2021) Response and oil degradation activities of a northeast Atlantic bacterial community to biogenic and synthetic surfactants. Microbiome 9(1):191. https://doi.org/10.1186/s40168-021-01143-5
Parthipan P, Preetham E, Machuca LL, Rahman PK, Murugan K, Rajasekar A (2017) Biosurfactant and Degradative Enzymes Mediated Crude Oil Degradation by Bacterium Bacillus subtilis A1. Front Microbiol 8:193. https://doi.org/10.3389/fmicb.2017.00193
Parus A, Ciesielski T, Woźniak-Karczewska M, Ślachciński M, Owsianiak M, Ławniczak Ł, Loibner AP, Heipieper HJ, Chrzanowski Ł (2023) Basic principles for biosurfactant-assisted (bio)remediation of soils contaminated by heavy metals and petroleum hydrocarbons: a critical evaluation of the performance of rhamnolipids. J Hazard Mater. https://doi.org/10.1016/j.jhazmat.2022.130171
Patel S, Homaei A, Patil S, Daverey A (2018) Microbial biosurfactants for oil spill remediation: pitfalls and potentials. Appl Microbiol Biotechnol 103(1):27–37. https://doi.org/10.1007/s00253-018-9434-2
Patowary K, Patowary R, Kalita MC, Deka S (2017) Characterization of biosurfactant produced during degradation of hydrocarbons using crude oil as sole source of carbon. Front Microbiol. https://doi.org/10.3389/fmicb.2017.00279
Phulpoto IA, Wang Y, Qazi MA, Hu B, Ndayisenga F, Yu Z (2021) Bioprospecting of rhamnolipids production and optimization by an oil-degrading Pseudomonas sp. S2WE isolated from freshwater lake. Bioresour Technol. https://doi.org/10.1016/j.biortech.2020.124601
Rahmati F, Asgari Lajayer B, Shadfar N, van Bodegom PM, van Hullebusch ED (2022) A Review on Biotechnological Approaches Applied for Marine Hydrocarbon Spills Remediation. Microorganisms. https://doi.org/10.3390/microorganisms10071289
Ren H, Zhou S, Wang B, Peng L, Li X (2020) Treatment mechanism of sludge containing highly viscous heavy oil using biosurfactant. Coll Surf Physicochem Eng Aspects. https://doi.org/10.1016/j.colsurfa.2019.124117
Saimmai A, Onlamool T, Sobhon V, Maneerat S (2012) An efficient biosurfactant-producing bacterium Selenomonas ruminantium CT2, isolated from mangrove sediment in south of Thailand. World J Microbiol Biotechnol 29(1):87–102. https://doi.org/10.1007/s11274-012-1161-8
Samadi S, Amani H, Najafpour GD, Kariminezhad H, Banaei A (2023) Investigating the potential of rhamnolipid as an eco-friendly surfactant for environmental protection in oil spill clean-up. Int J Environ Sci Technol 20(7):7277–7292. https://doi.org/10.1007/s13762-023-04973-y
Schreiberova O, Hedbavna P, Cejkova A, Jirku V, Masak J (2012) Effect of surfactants on the biofilm of Rhodococcus erythropolis, a potent degrader of aromatic pollutants. N Biotechnol 30(1):62–68. https://doi.org/10.1016/j.nbt.2012.04.005
Shahabi Rokni M, Halajnia A, Lakzian A, Housaindokht MR (2022) Sugar beet molasses bioconversion into biosurfactant: optimization and comparison with other carbon sources. Biomass Conver Biorefinery. https://doi.org/10.1007/s13399-022-03597-5
Sharma S, Datta P, Kumar B, Tiwari P, Pandey LM (2019) Production of novel rhamnolipids via biodegradation of waste cooking oil using Pseudomonas aeruginosa MTCC7815. Biodegradation 30(4):301–312. https://doi.org/10.1007/s10532-019-09874-x
Silva DP, Villela HDM, Santos HF, Duarte GAS, Ribeiro JR, Ghizelini AM, Vilela CLS, Rosado PM, Fazolato CS, Santoro EP, Carmo FL, Ximenes DS, Soriano AU, Rachid C, Vega Thurber RL, Peixoto RS (2021) Multi-domain probiotic consortium as an alternative to chemical remediation of oil spills at coral reefs and adjacent sites. Microbiome 9(1):118. https://doi.org/10.1186/s40168-021-01041-w
Soares Rodrigues CI, den Ridder M, Pabst M, Gombert AK, Wahl SA (2023) Comparative proteome analysis of different Saccharomyces cerevisiae strains during growth on sucrose and glucose. Scie Rep. https://doi.org/10.1038/s41598-023-29172-0
Su H, Kuang X, Ren Y, Luo L (2022a) Biostimulants promote biodegradation of n-hexadecane by Raoultella planticola: Generation of lipopeptide biosurfactants. J Environ Chem Eng. https://doi.org/10.1016/j.jece.2022.108382
Su Z, Yang S, Li M, Chen Y, Wang S, Yun Y, Li G, Ma T (2022b) Complete Genome Sequences of One Salt-Tolerant and Petroleum Hydrocarbon-Emulsifying Terribacillus saccharophilus Strain ZY-1. Front Microbiol. https://doi.org/10.3389/fmicb.2022.932269
Sun S, Su Y, Chen S, Cui W, Zhao C, Liu Q (2022) Bioremediation of oil-contaminated soil: Exploring the potential of endogenous hydrocarbon degrader Enterobacter sp. SAVR S-1. Appl Soil Ecol. https://doi.org/10.1016/j.apsoil.2022.104387
Techtmann SM, Santo Domingo J, Conmy R, Barron M (2023) Impacts of dispersants on microbial communities and ecological systems. Appl Microbiol Biotechnol 107(4):1095–1106. https://doi.org/10.1007/s00253-022-12332-z
Thio CW, Lim WH, Md Shah UK, Phang L-Y (2022) Palm kernel fatty acid distillate as substrate for rhamnolipids production using Pseudomonas sp LM19. Green Chem Lett Rev 15(1):83–92. https://doi.org/10.1080/17518253.2021.2023223
Vandana Das S (2023) Cell surface hydrophobicity and petroleum hydrocarbon degradation by biofilm-forming marine bacterium Pseudomonas furukawaii PPS-19 under different physicochemical stressors. J Hazard Mater. https://doi.org/10.1016/j.jhazmat.2023.131795
Varjani SJ, Upasani VN (2016) Core Flood study for enhanced oil recovery through ex-situ bioaugmentation with thermo- and halo-tolerant rhamnolipid produced by Pseudomonas aeruginosa NCIM 5514. Bioresour Technol 220:175–182. https://doi.org/10.1016/j.biortech.2016.08.060
Varjani SJ, Upasani VN (2017) Critical review on biosurfactant analysis, purification and characterization using rhamnolipid as a model biosurfactant. Bioresour Technol 232:389–397. https://doi.org/10.1016/j.biortech.2017.02.047
Varjani S, Upasani VN (2019) Evaluation of rhamnolipid production by a halotolerant novel strain of Pseudomonas aeruginosa. Bioresour Technol. https://doi.org/10.1016/j.biortech.2019.121577
Velioglu Z, Urek RO (2016) Physicochemical and structural characterization of biosurfactant produced by Pleurotus djamor in solid-state fermentation. Biotechnol Bioprocess Eng 21(3):430–438. https://doi.org/10.1007/s12257-016-0139-z
Vibhute AM, Sureshan KM (2020) How Far Are We in Combating Marine Oil Spills by Using Phase-Selective Organogelators? Chemsuschem 13(20):5343–5360. https://doi.org/10.1002/cssc.202001285
Wang SH, Tang TW, Wu E, Wang DW, Wang CF, Liao YD (2020a) Inhibition of bacterial adherence to biomaterials by coating antimicrobial peptides with anionic surfactant. Colloids Surf B Biointerfaces. https://doi.org/10.1016/j.colsurfb.2020.111364
Wang W, Lin J, Cheng J, Cui Z, Si J, Wang Q, Peng X, Turng L-S (2020b) Dual super-amphiphilic modified cellulose acetate nanofiber membranes with highly efficient oil/water separation and excellent antifouling properties. J Hazard Mater. https://doi.org/10.1016/j.jhazmat.2019.121582
Wang W, He Y, Wang B, Dong M, Zhang H, Shen C (2022) Experimental study on wax removal and viscosity reduction of waxy crude oil by Ochrobactrum intermedium. J Petrol Sci Eng. https://doi.org/10.1016/j.petrol.2022.110445
Wei F, Xu R, Rao Q, Zhang S, Ma Z, Ma Y (2023) Biodegradation of asphaltenes by an indigenous bioemulsifier-producing Pseudomonas stutzeri YWX-1 from shale oil in the Ordos Basin: Biochemical characterization and complete genome analysis. Ecotoxicol Environ Safety. https://doi.org/10.1016/j.ecoenv.2023.114551
Yan B, Wang X, Zhang X, Liu S, Li M, Ran R (2021) Novel dispersant based on the synergy of nickel hydroxide and sulfonated lignin for applications in oil spill remediation. J Environ Chem Eng. https://doi.org/10.1016/j.jece.2021.106607
Yesankar PJ, Pal M, Patil A, Qureshi A (2022) Microbial exopolymeric substances and biosurfactants as ‘bioavailability enhancers’ for polycyclic aromatic hydrocarbons biodegradation. Int J Environ Sci Technol 20(5):5823–5844. https://doi.org/10.1007/s13762-022-04068-0
Yin Q, Nie H, Nie M, Guo Y, Zhang B, Wang L, Wang Y, Bai X (2023) Rapid effective treatment of waxy oily sludge using a method of dispersion combined with biodegradation in a semi-fluid state. Environ Pollut. https://doi.org/10.1016/j.envpol.2022.120971
Zang T, Wu H, Yan B, Zhang Y, Wei C (2021) Enhancement of PAHs biodegradation in biosurfactant/phenol system by increasing the bioavailability of PAHs. Chemosphere. https://doi.org/10.1016/j.chemosphere.2020.128941
Zargar AN, Lymperatou A, Skiadas I, Kumar M, Srivastava P (2022) Structural and functional characterization of a novel biosurfactant from Bacillus sp. IITD106. J Hazard Mater. https://doi.org/10.1016/j.jhazmat.2021.127201
Zeng Z, Liu Y, Zhong H, Xiao R, Zeng G, Liu Z, Cheng M, Lai C, Zhang C, Liu G, Qin L (2018) Mechanisms for rhamnolipids-mediated biodegradation of hydrophobic organic compounds. Sci Total Environ 634:1–11. https://doi.org/10.1016/j.scitotenv.2018.03.349
Zhao F, Shi R, Cui Q, Han S, Dong H, Zhang Y (2017) Biosurfactant production under diverse conditions by two kinds of biosurfactant-producing bacteria for microbial enhanced oil recovery. J Petrol Sci Eng 157:124–130. https://doi.org/10.1016/j.petrol.2017.07.022
Zhao F, Yuan M, Lei L, Li C, Xu X (2021) Enhanced production of mono-rhamnolipid in Pseudomonas aeruginosa and application potential in agriculture and petroleum industry. Bioresour Technol. https://doi.org/10.1016/j.biortech.2020.124605
Zhu Z, Merlin F, Yang M, Lee K, Chen B, Liu B, Cao Y, Song X, Ye X, Li QK, Greer CW, Boufadel MC, Isaacman L, Zhang B (2022) Recent advances in chemical and biological degradation of spilled oil: A review of dispersants application in the marine environment. J Hazard Mater. https://doi.org/10.1016/j.jhazmat.2022.129260
Zock JP (2018) Some clues for studying long-term health effects of oil spills. Occup Environ Med 75(3):163. https://doi.org/10.1136/oemed-2017-104687
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This research was financially supported by the State Key Laboratory of Petroleum Pollution Control (Grant No. PPC2018014).
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MZ: conceptualization, methodology, data analysis, writing, software, writing-original draft, visualization and revision of original manuscript; HZ: conceptualization, supervision and review; WC, YS: review and editing; YS, SS, QL: project administration, review and editing; QL: funding acquisition, proposing concepts, data curation; All authors read and contributed the final manuscript.
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Zhu, M., Zhang, H., Cui, W. et al. Performance evaluation of rhamnolipid biosurfactant produced by Pseudomonas aeruginosa and its effect on marine oil-spill remediation. Arch Microbiol 206, 183 (2024). https://doi.org/10.1007/s00203-024-03903-x
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DOI: https://doi.org/10.1007/s00203-024-03903-x