Abstract
The stems and leaves of Panax notoginseng contain high saponins, but they are often discarded as agricultural waste. In this study, the predominant ginsenosides Rg1, Rc, and Rb2, presented in the stems and leaves of ginseng plants, were biotransformed into value-added rare ginsenosides F1, compound Mc1 (C-Mc1), and Rd2, respectively. A fungal strain YMS6 (Penicillium sp.) was screened from the soil as a biocatalyst with high selectivity for the deglycosylation of major ginsenosides. Under the optimal fermentation conditions, the yields of F1, C-Mc1, and Rd2 were 97.95, 68.64, and 79.58%, respectively. This study provides a new microbial resource for the selective conversion of protopanaxadiol-type and protopanaxatriol-type major saponins into rare ginsenosides via the whole-cell biotransformation and offers a solution for the better utilization of P. notoginseng waste.
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All data generated and analyzed during this study are available from the corresponding author on reasonable request.
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Acknowledgements
This work was supported by the Science Foundation Project of Guangxi Academy of Sciences (2020YBJ703, 2021YFJ1210 and CQ-D-2415), the Natural Science Foundation of Guangxi (2021GXNSFBA196087 and 2021AC19176), the Scientific and Technological Innovation Major Base of Guangxi (2022-36-Z06) and the Research Programs of the Science and Technology of Guangxi Zhuang Autonomous (AA22117015-3).
Funding
Science Foundation Project of Guangxi Academy of Sciences, 2020YBJ703, 2021YFJ1210, CQ-D-2415, Natural Science Foundation of Guangxi, 2021GXNSFBA196087, 2021AC19176, Scientific and Technological Innovation Major Base of Guangxi, 2022-36-Z06, Research Programs of the Science and Technology of Guangxi Zhuang Autonomous, AA22117015-3.
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M.D. and N.X. conceived and designed the experiment. M.D. performed most of the experimental work and wrote the manuscript. Y.C. performed experiments on compound identification and edited the manuscript. M.D., N.X., L.M., and J.L. are responsible for funding acquisition and manuscript editing. All authors contributed to the article and approved the submitted version.
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Diao, M., Chen, Y., Meng, L. et al. Biotransformation approach to produce rare ginsenosides F1, compound Mc1, and Rd2 from major ginsenosides. Arch Microbiol 206, 176 (2024). https://doi.org/10.1007/s00203-024-03893-w
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DOI: https://doi.org/10.1007/s00203-024-03893-w