Abstract
Tick-borne pathogens harm livestock production and pose a significant risk to public health. To combat these effects, it is necessary to identify the circulating pathogens to create effective control measures. This study identified Anaplasma and Ehrlichia species in ticks collected from livestock in the Kassena-Nankana Districts between February 2020 and December 2020. A total of 1550 ticks were collected from cattle, sheep and goats. The ticks were morphologically identified, pooled and screened for pathogens using primers that amplify a 345 bp fragment of the 16SrRNA gene and Sanger sequencing. The predominant tick species collected was Amblyomma variegatum (62.98%). From the 491 tick pools screened, 34 (6.92%) were positive for Ehrlichia and Anaplasma. The pathogens identified were Ehrlichia canis (4.28%), Ehrlichia minasensis (1.63%), Anaplasma capra (0.81%) and Anaplasma marginale (0.20%). This study reports the first molecular identification of the above-mentioned Ehrlichia and Anaplasma species in ticks from Ghana. With the association of human infections with the zoonotic pathogen A. capra, livestock owners are at risk of infections, calling for the development of effective control measures.
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Acknowledgements
The authors express their sincere thanks to the Navrongo Health Research Centre and the Parasitology Department of Noguchi Memorial Institute for Medical Research for their support and contribution.
Funding
This study was supported by the Uniformed Services University Center for Global Health Engagement (CGHE) through the Global Health Engagement Research Initiative (Grant number: GRANT12767296).
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SOA wrote the main manuscript. SOA, BOAB, REB, CAA and EB conducted the laboratory analysis. EB analyzed the data. SOA, VA, MS, SM, JCD, JAL, PKB, MDW, JWD and SKD designed the study. JAL, PKB, MDW and SKD supervised this study. All authors reviewed and approved the final manuscript.
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This study was approved by the University of Ghana Institutional Animal Care and Use Committee (UG-IACUC; UG-IACUC 001/19–20).
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The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, the Department of Defense, or the US Government. Opinions, interpretations, conclusions, and recommendations are those of the authors and are not necessarily endorsed by the US Army. The authors Joseph W. Diclaro II, James C. Dunford and Suzanne Mate are military service members or employees of the US Government. This work was prepared as part of their official duties. Title 17 USC §105 provides that “Copyright protection under this title is not available for any work of the United States Government”. Title 17 USC §101 defines US Government work as work prepared by a military service member or employee of the US Government as part of that person’s official duties.
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Addo, S.O., Baako, B.O.A., Bentil, R.E. et al. Molecular survey of Anaplasma and Ehrlichia species in livestock ticks from Kassena-Nankana, Ghana; with a first report of Anaplasma capra and Ehrlichia minasensis. Arch Microbiol 205, 92 (2023). https://doi.org/10.1007/s00203-023-03430-1
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DOI: https://doi.org/10.1007/s00203-023-03430-1