Abstract
A Gram-staining-positive actinobacteria strain, designated MY13T, was isolated from deep-sea sediment of the western Pacific Ocean and subjected to a taxonomic polyphasic investigation. Based on the results, cells were aerobic, irregular short rod, non-motile and non-spore-forming. Colonies were cream, circular, smooth, convex, opaque and 1.0–2.0 mm in diameter after growth on MZ2 medium at 40 °C for 72 h. Strain MY13T grew at 4–50 °C (optimum, 40 °C), pH 7–12 (pH 9) and 0.5–15% (w/v) NaCl (3.5%). The 16S rRNA gene sequence analysis revealed that strain MY13T is affiliated with the genus Nesterenkonia and closely related to Nesterenkonia populi GP10-3T (96.6%). Digital DNA–DNA hybridization (dDDH) values and average nucleotide identity (ANI) differentiated it from its closest relatives, with values ranging from 19.8% to 22.4% and 72.6% to 78.0%, respectively. Chemotaxonomic analysis showed that the major menaquinone of strain MY13T was MK-7; major cellular fatty acids were anteiso-C17:0, anteiso-C15:0 and iso-C16:0; whole-cell sugars were galactose and xylose; the peptidoglycan type was l-Lys-Gly-d-Asp; and polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unknown glycolipids, one unknown polar lipid and two unknown lipids. The G+C content of genomic DNA was 63.1 mol%. Based on the physiological, chemotaxonomic and phylogenetic data, strain MY13T is a novel species of the genus Nesterenkonia, for which the name Nesterenkonia sedimenti sp. nov. is proposed. The type strain is MY13T (= LMG 28111T = MCCC 1A09979T = JCM 19767T = CGMCC 1.12784T).
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This work was supported by grants from China Ocean Mineral Resources R&D Association (COMRA) Program, No. DY135-B2-01 and the Scientific Research Foundation of Third Institute of Oceanography, MNR, No. 2019011.
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FX performed isolation, deposition, and identification of strain MY13T. FX and SP performed phenotypic, physiological and biochemical analysis, and genome analysis. YZ analyzed the structure of peptidoglycan. YT and GZ designed research, analyzed data, and drafted the manuscript. All authors read and approved the final manuscript.
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Xie, F., Pei, S., Zhang, Y. et al. Nesterenkonia sedimenti sp. nov., isolated from marine sediment. Arch Microbiol 203, 6287–6293 (2021). https://doi.org/10.1007/s00203-021-02596-w
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DOI: https://doi.org/10.1007/s00203-021-02596-w