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Ruegeria alba sp. nov., Isolated from a Tidal Flat Sediment

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Abstract

A novel Gram-staining-negative, aerobic, rod-shaped, and white-colored bacterium designated as 1NDH52CT was isolated from a tidal flat sediment and its taxonomic position was determined using a polyphasic taxonomic approach. The microorganism was found to grow at 10–37 °C, pH 6.0–9.0, and in the presence of 0–2% (w/v) NaCl, and to hydrolyze gelatin and aesculin. The major cellular fatty acid of strain 1NDH52CT was summed feature 8 (C19:1 ω7c and/or C18:1 ω6c); the polar lipids comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an aminolipid, and a lipid; the respiratory quinone was ubiquinone-10. The 16S rRNA gene-based phylogenetic analysis showed that strain 1NDH52CT was closely related to members of the genus Ruegeria with the identity of 98.2% to the type strain Ruegeria pomeroyi DSM 15711T. The genome DNA G + C content of strain 1NDH52CT was 63.6%. The phylogenomic analysis indicated that strain 1NDH52CT formed an independent branch distinct from reference type strains of species within this genus. Digital DNA–DNA hybridization and average nucleotide identity values between strain 1NDH52CT and reference strains were, respectively, 19.1–41.5% and 78.3–91.3%, which are far below the thresholds of 70% and 95–96% for species definition, respectively, indicating that strain 1NDH52CT represents a novel genospecies of the genus Ruegeria. Based on phenotypic and genotypic data, strain 1NDH52CT is concluded to represent a novel species of the genus Ruegeria, for which the name Ruegeria alba sp. nov., is proposed. The type strain of the species is 1NDH52CT (= GDMCC 1.2382T = KCTC 82664T).

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Data Availability

All data generated during this study are publicly available from the GenBank database at www.ncbi.nlm.nih.gov/nuccore/OM570255.1/ and www.ncbi.nlm.nih.gov/nuccore/JAKOEM000000000.1/

Code Availability

Not applicable.

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Funding

This work was jointly supported by the Key Realm R&D Program of Guangdong Province (2020B0202080005), the Science and Technology Program of Guangdong Province (2021B1212050022), and the GDAS' Project of Science and Technology Development (2020GDASYL-20200103028).

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Authors and Affiliations

  1. Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Microbial Culture Collection Center (GDMCC), Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, 510070, People’s Republic of China

    Juan Du, Yang Liu, Tao Pei, Anzhang Li & Honghui Zhu

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  1. Juan Du
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  2. Yang Liu
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  3. Tao Pei
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Contributions

JD and HZ contributed to conceptualization and supervision and Validation; JD contributed to data curation; formal analysis, visualization, and writing—original draft; JD, AL, and HZ contributed to funding acquisition; JD, YL, TP, AL, and HZ contributed to investigation; JD and YL contributed to methodology; YL contributed to software; JD, YL, and HZ contributed to writing—review & editing.

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Correspondence to Honghui Zhu.

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Du, J., Liu, Y., Pei, T. et al. Ruegeria alba sp. nov., Isolated from a Tidal Flat Sediment. Curr Microbiol 79, 267 (2022). https://doi.org/10.1007/s00284-022-02968-5

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