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Assessment of fungal diversity in soil rhizosphere associated with Rhazya stricta and some desert plants using metagenomics

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Abstract

This study aimed to compare the fungal rhizosphere communities of Rhazya stricta, Enneapogon desvauxii, Citrullus colocynthis, Senna italica, and Zygophyllum simplex, and the gut mycobiota of Poekilocerus bufonius (Orthoptera, Pyrgomorphidae, “Usherhopper”). A total of 164,485 fungal reads were observed from the five plant rhizospheres and Usherhopper gut. The highest reads were in S. italica rhizosphere (29,883 reads). Species richness in the P. bufonius gut was the highest among the six samples. Ascomycota was dominant in all samples, with the highest reads in E. desvauxii (26,734 reads) rhizosphere. Sordariomycetes and Dothideomycetes were the dominant classes detected with the highest abundance in C. colocynthis and E. desvauxii rhizospheres. Aspergillus and Ceratobasidium were the most abundant genera in the R. stricta rhizosphere, Fusarium and Penicillium in the E. desvauxii rhizosphere and P. bufonius gut, Ceratobasidium and Myrothecium in the C. colocynthis rhizosphere, Aspergillus and Fusarium in the S. italica rhizosphere, and Cochliobolus in the Z. simplex rhizosphere. Aspergillus terreus was the most abundant species in the R. stricta and S. italica rhizospheres, Fusarium sp. in E. desvauxii rhizosphere, Ceratobasidium sp. in C. colocynthis rhizosphere, Cochliobolus sp. in Z. simplex rhizosphere, and Penicillium sp. in P. bufonius gut. The phylogenetic results revealed the unclassified species were related closely to Ascomycota and the species in E. desvauxii, S. italica and Z. simplex rhizospheres were closely related, where the species in the P. bufonius gut, were closely related to the species in the R. stricta, and C. colocynthis rhizospheres.

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Acknowledgment

This project was funded by the Deanship of Scientific Research (DSR) at King Abdulaziz University, Jeddah, under grant no. (HiCi 1-363-1434H). The authors, therefore, acknowledge with thanks DSR for technical and financial support.

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Authors and Affiliations

  1. Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia

    Samah O. Noor, Dhafer A. Al-Zahrani, Salah M. Abo-Aba, Ahmed M. Al-Hejin & Nabih A. Baeshen

  2. University of Jeddah, College of Sciences and Arts, Department of Biological Sciences, Al Kamel Province, Jeddah, Saudi Arabia

    Refaei M. Hussein

  3. Genetics and Cytology Dept. Genetic Engineering Division, National Resesrch Centre, Dokki, Cairo, Egypt

    Refaei M. Hussein

  4. University of Jeddah, College of Science, Department of Biological Sciences, Jeddah, Saudi Arabia

    Mohammed N. Baeshen

  5. Botany and Microbiology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt

    Tarek A. A. Moussa

  6. Department of Microbial Genetics, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Giza, Egypt

    Salah M. Abo-Aba

  7. Department of Integrative Biology, University of California, Berkeley, USA

    John P. Huelsenbeck

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  1. Samah O. Noor
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Correspondence to Tarek A. A. Moussa.

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Noor, S.O., Al-Zahrani, D.A., Hussein, R.M. et al. Assessment of fungal diversity in soil rhizosphere associated with Rhazya stricta and some desert plants using metagenomics. Arch Microbiol 203, 1211–1219 (2021). https://doi.org/10.1007/s00203-020-02119-z

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  • DOI: https://doi.org/10.1007/s00203-020-02119-z

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