Abstract
Key message
Inversions and translocations are the major chromosomal rearrangements involved in Vigna subgenera evolution, being Vigna vexillata the most divergent species. Centromeric repositioning seems to be frequent within the genus.
Abstract
Oligonucleotide-based fluorescence in situ hybridization (Oligo-FISH) provides a powerful chromosome identification system for inferring plant chromosomal evolution. Aiming to understand macrosynteny, chromosomal diversity, and the evolution of bean species from five Vigna subgenera, we constructed cytogenetic maps for eight taxa using oligo-FISH-based chromosome identification. We used oligopainting probes from chromosomes 2 and 3 of Phaseolus vulgaris L. and two barcode probes designed from V. unguiculata (L.) Walp. genome. Additionally, we analyzed genomic blocks among the Ancestral Phaseoleae Karyotype (APK), two V. unguiculata subspecies (V. subg. Vigna), and V. angularis (Willd.) Ohwi & Ohashi (V. subg. Ceratotropis). We observed macrosynteny for chromosomes 2, 3, 4, 6, 7, 8, 9, and 10 in all investigated taxa except for V. vexillata (L.) A. Rich (V. subg. Plectrotropis), in which only chromosomes 4, 7, and 9 were unambiguously identified. Collinearity breaks involved with chromosomes 2 and 3 were revealed. We identified minor differences in the painting pattern among the subgenera, in addition to multiple intra- and interblock inversions and intrachromosomal translocations. Other rearrangements included a pericentric inversion in chromosome 4 (V. subg. Vigna), a reciprocal translocation between chromosomes 1 and 5 (V. subg. Ceratotropis), a potential deletion in chromosome 11 of V. radiata (L.) Wilczek, as well as multiple intrablock inversions and centromere repositioning via genomic blocks. Our study allowed the visualization of karyotypic patterns in each subgenus, revealing important information for understanding intrageneric karyotypic evolution, and suggesting V. vexillata as the most karyotypically divergent species.
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Acknowledgements
We thank Embrapa Meio-Norte, IPK, and NPGS for supplying the seeds. We also thank CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), FACEPE (Fundação de Amparo à Ciência e Tecnologia de Pernambuco), and PAPq/UEMG for scholarships and/or financial support.
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This work was supported by CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) Grant no. 88881.189152/2018-01; CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) Grant no. 421968/2018-4, 313527/2017-2, 313944/2020-2, 433931/2018-3, and 442019/2019-0, and FACEPE (Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco) Grant no. APQ-0390-2.02/19.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by SD, FdOB, LdVM, VAdC, CM, ARdSO, GSdL, GTB, AFdC, and ACB-V. The first draft of the manuscript was written by SD, FdOB, LdVM, and ACBV, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Dias, S., de Oliveira Bustamante, F., do Vale Martins, L. et al. Translocations and inversions: major chromosomal rearrangements during Vigna (Leguminosae) evolution. Theor Appl Genet 137, 29 (2024). https://doi.org/10.1007/s00122-024-04546-8
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DOI: https://doi.org/10.1007/s00122-024-04546-8