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Intra- and interchromosomal rearrangements between cowpea [Vigna unguiculata (L.) Walp.] and common bean (Phaseolus vulgaris L.) revealed by BAC-FISH

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Abstract

Cowpea (Vigna unguiculata) is an annual legume grown in tropical and subtropical regions, which is economically relevant due to high protein content in dried beans, green pods, and leaves. In this work, a comparative cytogenetic study between V. unguiculata and Phaseolus vulgaris (common bean) was conducted using BAC-FISH. Sequences previously mapped in P. vulgaris chromosomes (Pv) were used as probes in V. unguiculata chromosomes (Vu), contributing to the analysis of macrosynteny between both legumes. Thirty-seven clones from P. vulgaris ‘BAT93’ BAC library, corresponding to its 11 linkage groups, were hybridized in situ. Several chromosomal rearrangements were identified, such as translocations (between BACs from Pv1 and Pv8; Pv2 and Pv3; as well as Pv2 and Pv11), duplications (BAC from Pv3), as well as paracentric and pericentric inversions (BACs from Pv3, and Pv4, respectively). Two BACs (from Pv2 and Pv7), which hybridized at terminal regions in almost all P. vulgaris chromosomes, showed single-copy signal in Vu. Additionally, 17 BACs showed no signal in V. unguiculata chromosomes. The present results demonstrate the feasibility of using BAC libraries in comparative chromosomal mapping and karyotype evolution studies between Phaseolus and Vigna species, and revealed several macrosynteny and collinearity breaks among both legumes.

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Abbreviations

BAC:

Bacterial artificial chromosome

CABMV:

Cowpea aphid born mosaic virus

CPSMV:

Cowpea severe mosaic virus

DAPI:

4′,6-Diamidino-2-phenylindole

FISH:

Fluorescent in situ hybridization

LG:

Linkage group

Mya:

Million years ago

rDNA:

Ribosomal DNA

SSC:

Saline-sodium citrate

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Acknowledgments

The authors thank Embrapa Meio-Norte (Teresina, Brazil) and Embrapa Arroz e Feijão (Santo Antônio de Goiás, Brazil) for supplying seeds, and also CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), CAPES (Coordenação de Pessoal de Nível Superior) and FACEPE (Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco) for financial support and fellowships.

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Authors and Affiliations

  1. Departamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE, 50670-420, Brazil

    Emanuelle Varão Vasconcelos, Ana Maria Benko-Iseppon & Ana Christina Brasileiro-Vidal

  2. Departamento de Botânica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Recife, PE, 50670-420, Brazil

    Artur Fellipe de Andrade Fonsêca & Andrea Pedrosa-Harand

  3. Campus Ciências Agrárias, Universidade Federal do Vale do São Francisco, Petrolina, PE, 56304-917, Brazil

    Kyria Cilene de Andrade Bortoleti

  4. Instituto Agronômico de Pernambuco, Av. General San Martin, 1371, Recife, PE, CEP: 50761-000, Brazil

    Antônio Félix da Costa

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  1. Emanuelle Varão Vasconcelos
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  2. Artur Fellipe de Andrade Fonsêca
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  3. Andrea Pedrosa-Harand
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Correspondence to Ana Christina Brasileiro-Vidal.

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Vasconcelos, E.V., de Andrade Fonsêca, A.F., Pedrosa-Harand, A. et al. Intra- and interchromosomal rearrangements between cowpea [Vigna unguiculata (L.) Walp.] and common bean (Phaseolus vulgaris L.) revealed by BAC-FISH. Chromosome Res 23, 253–266 (2015). https://doi.org/10.1007/s10577-014-9464-2

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