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Chromosome painting in meiosis reveals pairing of specific chromosomes in polyploid Solanum species

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Abstract

Analysis of chromosome pairing has been an important tool to assess the genetic similarity of homologous and homoeologous chromosomes in polyploids. However, it is technically challenging to monitor the pairing of specific chromosomes in polyploid species, especially for plant species with a large number of small chromosomes. We developed oligonucleotide-based painting probes for four different potato chromosomes. We demonstrate that these probes are robust enough to monitor a single chromosome throughout the prophase I of meiosis in polyploid Solanum species. Cultivated potato (Solanum tuberosum, 2n = 4x = 48) is an autotetraploid. We demonstrate that the four copies of each potato chromosome pair as a quadrivalent in 66–78% of the meiotic cells at the pachytene stage. Solanum demissum (2n = 6x = 72) is a hexaploid and has been controversial regarding its nature as an autopolyploid or allopolyploid. Interestingly, no hexavalent pairing was observed in meiosis. Instead, we observed three independent bivalents in 83–98% of the meiotic cells at late diakinesis and early metaphase I for the four chromosomes. These results suggest that S. demissum has evolved into a cytologically stable state with predominantly bivalent pairing in meiosis.

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Acknowledgements

We thank Drs. Bernd Friebe and Tomás Naranjo for valuable comments on the manuscript.

Funding

This research was supported partially by National Science Foundation (NSF) grant ISO-1237969 and MSU startup funds to J.J.

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Authors and Affiliations

  1. Horticulture Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China

    Li He

  2. Department of Horticulture, University of Wisconsin-Madison, Madison, WI, 53706, USA

    Li He, Guilherme T. Braz & Jiming Jiang

  3. Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA

    Li He, Guilherme T. Braz & Jiming Jiang

  4. Department of Horticulture, Michigan State University, East Lansing, MI, 48824, USA

    Li He, Guilherme T. Braz & Jiming Jiang

  5. Departmento de Biologia, Universidade Federal de Lavras, Lavras, MG, 37200, Brazil

    Guilherme T. Braz & Giovana A. Torres

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  1. Li He
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  2. Guilherme T. Braz
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  3. Giovana A. Torres
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Contributions

J.J. conceived the research, L.H. and G.T.B. and conducted FISH experiments. L.H., G.T.B., G.A.T., and J.J. analyzed data. J.J. wrote the article.

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Correspondence to Jiming Jiang.

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The authors declare that they have no conflict of interest.

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He, L., Braz, G.T., Torres, G.A. et al. Chromosome painting in meiosis reveals pairing of specific chromosomes in polyploid Solanum species. Chromosoma 127, 505–513 (2018). https://doi.org/10.1007/s00412-018-0682-9

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