Abstract
Key Message
QMS-5B, a major QTL for photo-thermo-sensitive genic male sterility in wheat, was fine mapped in a 2.15 Mb region harboring a serine/threonine protein kinase gene TraesCS5B03G0887500, which was the most likely candidate gene.
Abstract
Genic male sterility is an essential trait in the utilization of heterosis and hybrid seed production for wheat. Currently, genic male sterile genes have been reported in wheat mutants, but the sterile genes controlling photo-thermo-sensitive genic male sterility in wheat have not been studied systematically. Here, 235 doubled haploid lines derived from a cross between photo-thermo-sensitive genic male sterile line BS462 and its restorer line CP279 were used to map male sterile gene by GenoBaits® Wheat 100 K Panel, bulked segregant exome sequencing (BSE-Seq) and wheat 660 K array. As a result, the major stable QTL on chromosome 5B, QMS-5B, was identified in all four environments, accounting for 7.3–36.4% of the phenotypic variances. Ulteriorly, QMS-5B was delimited to an approximate 2.15 Mb physical interval between KASP-5B5 and KASP-5B6 using kompetitive allele-specific PCR (KASP) markers. Within the interval, twenty-nine high-confidence genes were predicted according to Chinese Spring RefSeq v2.1. TraesCS5B03G0887500, encoding a serine/threonine protein kinase, was identified as the most likely candidate gene for QMS-5B based on weighted gene co-expression network analysis. Expression analysis confirmed that TraesCS5B03G0887500 was significantly differentially expressed in anthers of BS462 and CP279 at different stages under fertile and sterile environments. In addition, flanking KASP marker KASP-5B6 can effectively genotype male sterile lines and restorer lines, and can be used for molecular marker-assisted selection. This study provides insights into for exploring the mechanism of photo-thermo-sensitive genic male sterility in wheat.
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Abbreviations
- Bns:
-
Binucleate stage
- BSE-Seq:
-
Bulked segregant exome sequencing
- DH:
-
Doubled haploid
- Ds:
-
Dyad stage
- KASP:
-
Kompetitive allele-specific PCR
- Mmcs:
-
Microspore mother cell stage
- QTL:
-
Quantitative trait locus
- SNP:
-
Single nucleotide polymorphism
- Tns:
-
Trinucleate stage
- Ts:
-
Tetrad stage
- Uns:
-
Uninucleate stage
- WGCNA:
-
Weighted gene co-expression network analysis
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Acknowledgements
The authors gratefully thank MolBreeding Biotech Ltd. for their help with GenoBaits® Wheat 100K Panel and BSE-Seq analysis. And the authors gratefully thank China Golden Marker Biotech Co., Ltd. for their help with wheat 660K SNP array genotyping.
Funding
This study was sponsored by the National Natural Science Foundation for Young Scientists of China (32001534); Beijing Municipal Natural Science Foundation (6232010); Outstanding Scientist Cultivation Program of BAAFS (JKZX202210); National Natural Science Foundation of China (32272108); Foundation for Youths of BAAFS (QNJJ202212).
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LPZ, XYS and CPZ conceived and designed the study. FQN and ZHL performed the experiments, analyzed the data and wrote the article with contributions from all of the authors. FTZ and SHY provided experimental materials. YML, HZ and HSZ participated in field trials. JFB and YJL revised and polished this manuscript. All authors have read and agreed to the published version of the manuscript.
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Niu, F., Liu, Z., Zhang, F. et al. Identification and validation of major-effect quantitative trait locus QMS-5B associated with male sterility in photo-thermo-sensitive genic male sterile wheat. Theor Appl Genet 136, 257 (2023). https://doi.org/10.1007/s00122-023-04500-0
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DOI: https://doi.org/10.1007/s00122-023-04500-0