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Genetic mapping and analysis of candidate leaf color genes in common winter wheat (Triticum aestivum L.)

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Abstract

Leaf color-related genes play key roles in chloroplast development and photosynthetic pigment biosynthesis and affect photosynthetic efficiency and grain yield in crops. In this study, a recessive homozygous individual displaying yellow leaf color (yl1) was identified in the progeny population derived from a cross between wheat cultivars Xingmai1 (XM1) and Yunong3114 (YN3114). Phenotypic identification showed that yl1 exhibited the yellow character state over the entire growth period. Compared with XM1, yl1 plants had significantly lower chlorophyll content and net photosynthetic rate, and similar results were found between the green-type lines and yellow-type lines in the BC2F3 XM1 × yl1 population. Gene mapping via the bulked segregant exome capture sequencing (BSE-seq) method showed that the target gene TaYL1 was located within the region of 582,556,971–600,837,326 bp on chromosome 7D. Further analysis by RNA-seq suggested TraesCS7D02G469200 as a candidate gene for yellow leaf color in common wheat, which encodes a protein containing the AP2 domain. Moreover, comparative transcriptome profiling revealed that most differentially expressed genes (DEGs) were enriched in chlorophyll metabolism and photosynthesis pathways. Together, these results indicate that TaYL1 potentially affects chlorophyll synthesis and photosynthesis. This study further elucidates the biological mechanism of chlorophyll synthesis, metabolism, and photosynthesis in wheat and provides a theoretical basis for high photosynthetic efficiency in wheat breeding.

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Data used in the manuscript as electronic supplementary materials. The research materials used in this study can be obtained by request.

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Funding

This work was funded by the National Natural Science Foundation (31971947 and 32072057), Henan Major Science and Technology Projects (201300110800 and 201300111600) and Key Scientific and Technological Project of Henan Province (212102110246 and 222102110026) of China.

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Authors and Affiliations

  1. National Key Laboratory of Wheat and Maize Crop Science/CIMMYT-China Wheat and Maize Joint Research Center/Agronomy College, Henan Agricultural University, 15 Longzihu College District, Zhengzhou, 450046, China

    Lei Zhao, Yulu Yang, Pengyu Hu, Qi Qiao, Guoguo Lv, Jiaqi Li, Lu Liu, Jiajie Wei, Yan Ren, Zhongdong Dong & Feng Chen

Authors
  1. Lei Zhao
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  2. Yulu Yang
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  3. Pengyu Hu
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  4. Qi Qiao
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  5. Guoguo Lv
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  6. Jiaqi Li
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  7. Lu Liu
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  9. Yan Ren
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  10. Zhongdong Dong
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  11. Feng Chen
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Contributions

LZ designed the experiment and organized the manuscript. LZ, YY, PH, and QQ performed BSE-Seq analysis and identified the function of TaYL1 gene. GL, JL, LL, and JW investigated agronomic traits. YR and ZD provided experimental materials. FC participated in the supervision of the overall work and contributed to the finalization of the manuscript. All authors read and approved the manuscript.

Corresponding author

Correspondence to Feng Chen.

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Zhao, L., Yang, Y., Hu, P. et al. Genetic mapping and analysis of candidate leaf color genes in common winter wheat (Triticum aestivum L.). Mol Breeding 43, 48 (2023). https://doi.org/10.1007/s11032-023-01395-z

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