Skip to main content

Advertisement

SpringerLink
Log in

Cytogenetic and genomic characterization of a novel tall wheatgrass-derived Fhb7 allele integrated into wheat B genome

  • Original Article
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Key message

We identified and integrated the novel FHB-resistant Fhb7The2 allele into wheat B genome and made it usable in both common and durum wheat breeding programs without yellow flour linkage drag.

Abstract

A novel tall wheatgrass-derived (Thinopyrum elongatum, genome EE) Fhb7 allele, designated Fhb7The2, was identified and integrated into the wheat B genome through a small 7B–7E translocation (7BS·7BL–7EL) involving the terminal regions of the long arms. Fhb7The2 conditions significant Type II resistance to Fusarium head blight (FHB) in wheat. Integration of Fhb7The2 into the wheat B genome makes this wild species-derived FHB resistance gene usable for breeding in both common and durum wheat. By contrast, other Fhb7 introgression lines involving wheat chromosome 7D can be utilized only in common wheat breeding programs, not in durum wheat. Additionally, we found that Fhb7The2 does not have the linkage drag of the yellow flour pigment gene that is tightly linked to the decaploid Th. ponticum-derived Fhb7 allele Fhb7Thp. This will further improve the utility of Fhb7The2 in wheat breeding. DNA sequence analysis identified 12 single nucleotide polymorphisms (SNPs) in Fhb7The2, Fhb7Thp, and another Th. elongatum-derived Fhb7 allele Fhb7The1, which led to seven amino acid conversions in Fhb7The2, Fhb7Thp, and Fhb7The1, respectively. However, no significant variation was observed in their predicted protein configuration as a glutathione transferase. Diagnostic DNA markers were developed specifically for Fhb7The2. The 7EL segment containing Fhb7The2 in the translocation chromosome 7BS·7BL–7EL exhibited a monogenic inheritance pattern in the wheat genetic background. This will enhance the efficacy of marker-assisted selection for Fhb7The2 introgression, pyramiding, and deployment in wheat germplasm and varieties.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

References

  • AACCI (2010) Approved methods of analysis, 26-50.01. Brabender Quadrumat Jr. (Quadruplex) method. Cereals & grains association, St. Paul, MN, USA. https://doi.org/10.1094/AACCIntMethod-26-50.01

  • AACCI (2012) Approved methods of analysis, 14-60.01. Total carotenoid content of cereal grains and flours. Cereals & grains association, St. Paul, MN, USA. https://www.cerealsgrains.org/resources/Methods/Methods/14-60.pdf

  • Alaux M, Rogers J, Letellier T, Flores R, Alfama F, Pommier C, Mohellibi N, Durand S, Kimmel E, Michotey C et al (2018) Linking the international wheat genome sequencing consortium bread wheat reference genome sequence to wheat genetic and phenomic data. Genome Biol 19:111

    Article  Google Scholar 

  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410

    Article  CAS  Google Scholar 

  • Buerstmayr H, Stierschneider M, Steiner B, Lemmens M, Griesser M, Nevo E, Fahima T (2003) Variation for resistance to head blight caused by F. graminearum in wild emmer originating from Israel. Euphytica 130:17–23

    Article  Google Scholar 

  • Cai X, Chen PD, Xu SS, Oliver RE, Chen X (2005) Utilization of alien genes to enhance Fusarium head blight resistance in wheat: a review. Euphytica 142:309–318

    Article  Google Scholar 

  • Cainong JC, Bockus WW, Feng Y, Chen P, Qi L, Sehgal SK, Danilova TV, Koo D-H, Friebe B, Gill BS (2015) Chromosome engineering, mapping, and transferring of resistance to Fusarium head blight disease from Elymus tsukushiensis into wheat. Theor Appl Genet 128:1019–1027

    Article  CAS  Google Scholar 

  • Ceoloni C, Forte P, Kuzmanovic L, Tundo S, Moscetti I, De Vita P, Virili ME, D’Ovidio R (2017) Cytogenetic mapping of a major locus for resistance to Fusarium head blight and crown rot of wheat on Thinopyrum elongatum 7EL and its pyramiding with valuable genes from a Th. ponticum homoeologous arm onto bread wheat 7DL. Theor Appl Genet 130:2005–2024

    Article  CAS  Google Scholar 

  • Chen X, Faris J, Hu J, Stack R, Adhikari T, Elias E, Kianian S, Cai X (2007) Saturation and comparative mapping of a major Fusarium head blight resistance QTL in tetraploid wheat. Mol Breed 19:113–124

    Article  CAS  Google Scholar 

  • CIE (1986) Colorimetry (Publication 15.2), 2nd edn. Commission Internationale de L’Éclairage, Vienna, Austria

  • Corpet F (1988) Multiple sequence alignment with hierarchical clustering. Nucl Acids Res 16(22):10881–10890

    Article  CAS  Google Scholar 

  • Danilova TV, Friebe B, Gill BS (2012) Single-copy gene fluorescence in situ hybridization and genome analysis: Acc-2 loci mark evolutionary chromosomal rearrangements in wheat. Chromosoma 121:597–611

    Article  CAS  Google Scholar 

  • Danilova TV, Poland J, Friebe B (2019) Production of a complete set of wheat–barley group-7 chromosome recombinants with increased grain β-glucan content. Theor Appl Genet 132:3129–3141. https://doi.org/10.1007/s00122-019-03411-3

    Article  CAS  Google Scholar 

  • Danilova TV, Akhunova AR, Akhunov ED, Friebe B, Gill BS (2017) Major structural genomic alterations can be associated with hybrid speciation in Aegilops markgrafii (Triticeae). Plant J 92:317–330

    Article  CAS  Google Scholar 

  • Fedak G, Chi D, Wolfe D, Ouellet T, Cao WG, Han FP, Xue A (2021) Transfer of fusarium head blight resistance from Thinopyrum elongatum to bread wheat cultivar Chinese spring. Genome 64:997–1008

    Article  CAS  Google Scholar 

  • Forte P, Virili ME, Kuzmanović L, Moscetti I, Andrea Gennaro A, D’Ovidio R, Ceoloni C (2014) A novel assembly of Thinopyrum ponticum genes into the durum wheat genome: pyramiding Fusarium head blight resistance onto recombinant lines previously engineered for other beneficial traits from the same alien species. Mol Breed 34:1701–1716

    Article  CAS  Google Scholar 

  • Grewal S, Hubbart-Edwards S, Yang C, Devi U, Baker L, Heath J, Scholefield SAD, Howells C, Yarde J, Isaac P, King IP, King J (2020) Rapid identification of homozygosity and site of wild relative introgressions in wheat through chromosome-specific KASP genotyping assays. Plant Biotechnol J 18:743–755

    Article  CAS  Google Scholar 

  • Guo J, Zhang X, Hou Y, Cai J, Shen X, Zhou T, Xu H, Ohm HW, Wang H, Li A, Han F, Wang H, Kong L (2015) High-density mapping of the major FHB resistance gene Fhb7 derived from Thinopyrum ponticum and its pyramiding with Fhb1 by marker-assisted selection. Theor Appl Genet 128:2301–2316

    Article  Google Scholar 

  • Haile JK, N’Diaye A, Walkowiak S, Nilsen KT, Clarke JM, Kutcher HR, Steiner B, Buerstmayr H, Pozniak CJ (2019) Fusarium head blight in durum wheat: recent status, breeding directions and future prospects. Phytopathology 109:1664–1675

    Article  CAS  Google Scholar 

  • Kato A, Lamb JC, Birchler JA (2004) Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize. Proc Natl Acad Sci USA 101:13554–13559

    Article  CAS  Google Scholar 

  • Kato A, Albert PS, Vega JM, Birchler JA (2006) Sensitive fluorescence in situ hybridization signal detection in maize using directly labeled probes produced by high concentration DNA polymerase nick translation. Biotech Histochem 81:71–78

    Article  Google Scholar 

  • Kim NS, Armstrong K, Knott DR (1993) Molecular detection of Lophopyrum chromatin in wheat–Lophopyrum recombinants and their use in the physical mapping of chromosome 7D. Theor Appl Genet 85:561–567

    Article  CAS  Google Scholar 

  • Long Y, Chao WS, Ma G, Xu SS, Qi L (2017) An innovative SNP genotyping method adapting to multiple platforms and throughputs. Theor Appl Genet 130:597–607

    Article  CAS  Google Scholar 

  • McArthur RI, Zhu XW, Oliver RE et al (2012) Homoeology of Thinopyrum junceum and Elymus rectisetus chromosomes to wheat and disease resistance conferred by the Thinopyrum and Elymus chromosomes in wheat. Chrom Res 20:699–715

    Article  CAS  Google Scholar 

  • McIntyre CL, Pereira S, Moran LB, Appels R (1990) New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat. Genome 33:635–640

    Article  CAS  Google Scholar 

  • Niu Z, Klindworth DL, Friesen TL, Chao S, Jin Y, Cai X, Xu SS (2011) DNA marker-assisted chromosome engineering of wheat carrying stem rust resistance gene Sr39 derived from Aegilops speltoides. Genetics 187:1011–1021

    Article  CAS  Google Scholar 

  • Niu Z, Klindworth DL, Yu G, Friesen TL, Chao S, Jin Y, Cai X, Ohm J-B, Rasmussen JB, Xu SS (2014) Development and characterization of wheat lines carrying stem rust resistance gene Sr43 derived from Thinopyrum ponticum. Theor Appl Genet 127:969–980

    Article  CAS  Google Scholar 

  • Oliver RE, Stack RW, Miller JD, Cai X (2007) Reaction of wild emmer wheat accessions to Fusarium head blight. Crop Sci 47:893–897

    Article  Google Scholar 

  • Oliver RE, Cai X, Friesen TL, Halley S, Stack RW, Xu SS (2008) Evaluation of Fusarium head blight resistance in tetraploid wheat (Triticum turgidum L.). Crop Sci 48:213–222

    Article  Google Scholar 

  • Otto CD, Kianian SF, Elias EM, Stack RW, Joppa LR (2002) Genetic dissection of a major Fusarium head blight QTL in tetraploid wheat. Plant Mol Biol 48:625–632

    Article  CAS  Google Scholar 

  • Qi LL, Pumphrey MO, Friebe B, Chen PD, Gill BS (2008) Molecular cytogenetic characterization of alien introgressions with gene Fhb3 for resistance to Fusarium head blight disease of wheat. Theor Appl Genet 117:1155–1166

    Article  CAS  Google Scholar 

  • Shen X, Kong L, Ohm H (2004) Fusarium head blight resistance in hexaploid wheat (Triticum aestivum)-Lophopyrum genetic lines and tagging of the alien chromatin by PCR markers. Theor Appl Genet 108:808–813

    Article  CAS  Google Scholar 

  • Szabó-Hevér Á, Zhang Q, Friesen TL, Zhong S, Elias EM, Cai X, Jin Y, Faris JD, Chao S, Xu SS (2018) Genetic diversity and resistance to Fusarium head blight in synthetic hexaploid wheat derived from Aegilops tauschii and diverse Triticum turgidum subspecies. Front Plant Sci 9:1829

    Article  Google Scholar 

  • Wang S, Wong D, Forrest K, Allen A, Huang BE, Maccaferri M, Salvi S, Milner SG, Cattivelli L, Mastrangelo AM, Whan A, Stephen S, Barker G, Wieseke R, Plieske J, International Wheat Genome Sequencing Consortium, Lillemo M, Mather D, Appels R, Dolferus R, Brown-Guedira G, Korol A, Akhunova AR, Feuillet C, Salse J, Morgante M, Pozniak C, Luo MC, Dvorak J, Morell M, Dubcovsky J, Ganal M, Tuberosa R, Lawley C, Mikoulitch I, Cavanagh C, Edwards KJ, Hayden M, Akhunov E (2014) Characterization of polyploid wheat genomic diversity using a high-density 90,000 single nucleotide polymorphism array. Plant Biotechnol J 12:787–796

  • Wang H, Sun S, Ge W, Zhao L, Hou B, Wang K, Lyu Z, Chen L, Xu S, Guo J, Li M, Su P, Li X, Wang G, Bo C, Fang X, Zhuang W, Cheng X, Wu J, Dong L, Chen W, Li W, Xiao G, Zhao J, Hao Y, Xu Y, Gao Y, Liu W, Liu Y, Yin H, Li J, Li X, Zhao Y, Wang X, Ni F, Ma X, Li A, Xu SS, Bai G, Nevo E, Gao C, Ohm H, Kong L (2020) Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat. Science 368:eaba5435

  • Wilson WW, McKee G, Nganje W, Dahl B, Bangsund D (2017) Economic impact of USWBSI’s scab initiative to reduce FHB. Agribusiness and applied economics No. 774. North Dakota State Univ

  • Zhang W, Cai X (2019) Alien introgression and breeding of synthetic wheat. In: Ordon F, Friedt W (eds) Advances in breeding techniques for cereal crops. Burleigh dodds science publishing, Cambridge, pp 3–54

    Chapter  Google Scholar 

  • Zhang W, Dubcovsky J (2008) Association between allelic variation at the Phytoene synthase 1 gene and yellow pigment content in the wheat grain. Theor Appl Genet 116:635–645

    Article  CAS  Google Scholar 

  • Zhang W, Lukaszewski A, Kolmer J, Soria M, Goyal S, Dubcovsky J (2005) Molecular characterization of durum and common wheat recombinant lines carrying leaf rust resistance (Lr19) and yellow pigment (Y) genes from Lophopyrum ponticum. Theor Appl Genet 111:573–582

    Article  CAS  Google Scholar 

  • Zhang X, Shen X, Hao Y, Cai J, Ohm HW, Kong L (2011) A genetic map of Lophopyrum ponticum chromosome 7E, harboring resistance genes to Fusarium head blight and leaf rust. Theor Appl Genet 122:263–270

    Article  CAS  Google Scholar 

  • Zhang Q, Axtman JE, Faris JD, Chao S, Zhang Z, Friesen TL, Zhong S, Cai X, Elias EM, Xu SS (2014) Identification and molecular mapping of quantitative trait loci for Fusarium head blight resistance in emmer and durum wheat using a single nucleotide polymorphism-based linkage map. Mol Breed 34:1677–1687

    Article  CAS  Google Scholar 

  • Zhang W, Cao Y, Zhang M, Zhu X, Ren S, Long Y, Gyawali Y, Chao S, Xu S, Cai X (2017) Meiotic homoeologous recombination-based alien gene introgression in the genomics era of wheat. Crop Sci 57:1189–1198

    Article  CAS  Google Scholar 

  • Zhang W, Zhang M, Zhu X, Cao Y, Sun Q, Ma G, Chao S, Yan C, Xu S, Cai X (2018) Molecular cytogenetic and genomic analyses reveal new insights into the origin of the wheat B genome. Theor Appl Genet 131:365–375

    Article  CAS  Google Scholar 

  • Zhang M, Zhang W, Zhu X, Sun Q, Yan C, Xu SS, Fiedler J, Cai X (2020) Dissection and physical mapping of wheat chromosome 7B by inducing meiotic recombination with its homoeologues in Aegilops speltoides and Thinopyrum elongatum. Theor Appl Genet 133:3455–3467

    Article  CAS  Google Scholar 

  • Zhu X, Zhong S, Chao S, Gu YQ, Kianian SF, Elias E, Cai XW (2016) Toward a better understanding of the genomic region harboring Fusarium head blight resistance QTL Qfhs.ndsu-3AS in durum wheat. Theor Appl Genet 129:31–43

    Article  CAS  Google Scholar 

  • Zhu T, Wang L, Rimbert H, Rodriguez JC, Deal KR, De Oliveira R, Choulet F, Keeble-Gagnère G, Tibbits J, Rogers J, Eversole K, Appels R, Gu YQ, Mascher M, Dvorak J, Luo M (2021) Optical maps refine the bread wheat Triticum aestivum cv Chinese Spring genome assembly. Plant J 107:303–314

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank Mary Osenga for her assistance in high-throughput SNP genotyping.

Funding

This project has been supported by Agriculture and Food Research Initiative Competitive Grant no. 2013-67013-21121 and 2019-67013-35750 from the USDA National Institute of Food and Agriculture, and the US Wheat & Barley Scab Initiative Grant no. 59-0206-7-002.

Author information

Author notes

  1. Wei Zhang and Tatiana Danilova have contributed to this work equally.

Authors and Affiliations

  1. Shanxi Key Laboratory of Minor Crop Germplasm Innovation and Molecular Breeding, Shanxi Agricultural University, Taiyuan, 030031, China

    Wei Zhang

  2. Departments of Plant Sciences, North Dakota State University, Fargo, ND, 58108, USA

    Wei Zhang, Mingyi Zhang, Shuangfeng Ren, Xianwen Zhu & Qijun Zhang

  3. Wheat, Sorghum & Forage Research Unit, USDA-ARS, Lincoln, NE, 68583, USA

    Tatiana Danilova, Jeffrey Boehm Jr. & Xiwen Cai

  4. Department of Plant Pathology, North Dakota State University, Fargo, ND, 58108, USA

    Shaobin Zhong

  5. Cereal Crops Research Unit, Edward T. Schafer Agricultural Research Center, USDA-ARS, Fargo, ND, 58102, USA

    Linda Dykes & Jason Fiedler

  6. Crop Improvement and Genetics Research Unit, Western Regional Research Center, USDA-ARS, Albany, CA, 94710, USA

    Steven Xu

  7. Department of Agronomy and Horticulture, University of Nebraska, Lincoln, NE, 68583, USA

    Katherine Frels, Jeffrey Boehm Jr. & Xiwen Cai

  8. Department of Plant Pathology, University of Nebraska, Lincoln, NE, 68583, USA

    Stephen Wegulo

Authors
  1. Wei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tatiana Danilova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mingyi Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shuangfeng Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xianwen Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qijun Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shaobin Zhong
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Linda Dykes
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Jason Fiedler
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Steven Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Katherine Frels
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Stephen Wegulo
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Jeffrey Boehm Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Xiwen Cai
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

WZ contributed to marker development and analysis, cloning, recombinant production and analysis, and data preparation and analysis. TD performed FISH/GISH analysis, SNP marker development, and involved in manuscript preparation. MZ contributed to recombinant production and analysis and SNP assays. SR performed FHB disease evaluation. XZ participated in crossing and chromosome-specific marker analysis for recombinant production. QZ was involved in DNA marker development. SZ was involved in FHB disease evaluation. L.D. performed flour color analysis and contributed to manuscript preparation. JF contributed SNP assays. SX contributed to experiment planning, interpretation, and manuscript preparation. KF contributed to experimental results interpretation and critical revision of the manuscript. SW contributed to FHB disease evaluation and manuscript preparation. JB: contributed to the DNA marker development and critical revision of the manuscript. XC designed and coordinated this work, and was involved in crosses, data analysis and interpretation, and led the manuscript preparation.

Corresponding author

Correspondence to Xiwen Cai.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest. The authors have no relevant financial or non-financial interest to disclose.

Additional information

Communicated by Lingrang Kong.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

122_2022_4228_MOESM1_ESM.tif

Supplementary file1. Figure 1. FISH patterns of DS 7E(7B), WGC002 (7BS·7BL-7E), and WGC001 (7BS·7BL-7EL-7BL). Th. elongatum chromatin, (GAA)n repeat, and pSc119.2 repeat are painted green, white, and red, respectively. Chromosomes are counterstained orange. (TIF 18890 KB)

122_2022_4228_MOESM2_ESM.docx

Supplementary file2 Figure 2. Coding DNA sequence alignments of Fhb7Thp, Fhb7The1 (Wang et al. 2020) and Fhb7The2 (DOCX 25 KB)

122_2022_4228_MOESM3_ESM.docx

Supplementary file3 Figure 3. The predicted protein models of Fhb7Thp , Fhb7The1 and Fhb7The2 based on the crystal structure of glutathione transferase GSTFuA3 using the SWISS-MODEL. (DOCX 335 KB)

122_2022_4228_MOESM4_ESM.xlsx

Supplementary file4 Table 1. Wheat 90K SNP genotyping data of CS chromosome 7B, 7E of DS 7E(7B), WGC002, and WGC001. (XLSX 34 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhang, W., Danilova, T., Zhang, M. et al. Cytogenetic and genomic characterization of a novel tall wheatgrass-derived Fhb7 allele integrated into wheat B genome. Theor Appl Genet 135, 4409–4419 (2022). https://doi.org/10.1007/s00122-022-04228-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-022-04228-3

Search

Navigation