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GW10, a member of P450 subfamily regulates grain size and grain number in rice

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Abstract

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A quantitative trait locus GW10 is located on Chromosome 10 by map-based cloning, which encodes a P450 Subfamily protein. The GW10 regulates grain size and grain number in rice involved in the BR pathway.

Abstract

Grain size and grain number play extremely important roles in rice grain yield. Here, we identify GW10, which encodes a P450 subfamily protein and controls grain size and grain number by using Lemont (tropical japonica) as donor parent and HJX74 (indica) as recipient parent. The GW10 locus was mapped into a 14.6 kb region in HJX74 genomic on the long arm of chromosome 10. Lower expression of the gw10 in panicle is contributed to the shorter and narrower rice grain, and the increased number of grains per panicle. In contrast, overexpression of GW10 is contributed to longer and wider rice grain. Furthermore, the higher expression levels of some of the brassinosteroid (BR) biosynthesis and response genes are associated with the NIL-GW10. The sensitivity of the leaf angle to exogenous BR in NIL-GW10 is lower than that in NIL-gw10 and in the KO-GW10, which implied that the GW10 should involve in the brassinosteroid-mediated regulation of rice grain size and grain number.

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Abbreviations

cM:

Centimorgan

QTL:

Quantitative trait loci

SSR:

Simple sequence repeats

SSSL:

Single segment substitution line

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Funding

This work was supported by the Key Projects of Basic Research and Applied Basic Research of Guangdong Province (2019B030302006) and the National Natural Science Foundation of China (32072040).

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Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Plant Molecular Breeding, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China

    Penglin Zhan, Xin Wei, Zhili Xiao, Xiaoling Wang, Shuaipeng Ma, Shaojun Lin, Fangping Li, Suhong Bu, Zupei Liu, Haitao Zhu, Guifu Liu, Guiquan Zhang & Shaokui Wang

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  1. Penglin Zhan
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  2. Xin Wei
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  3. Zhili Xiao
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  4. Xiaoling Wang
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  5. Shuaipeng Ma
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  6. Shaojun Lin
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  7. Fangping Li
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  9. Zupei Liu
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  10. Haitao Zhu
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  11. Guifu Liu
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  12. Guiquan Zhang
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  13. Shaokui Wang
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Contributions

SW and GZ designed and supervised works. PZ performed most of the experiments, analyzed experimental data and prepared the draft of manuscript. XW, XW, ZX, SM, SL and FL conducted a part of experiments. SB, ZL, HZ and GL developed the materials. SW wrote the paper. All authors read and approved the final manuscript.

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Correspondence to Guiquan Zhang or Shaokui Wang.

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Communicated by Takuji Sasaki.

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Zhan, P., Wei, X., Xiao, Z. et al. GW10, a member of P450 subfamily regulates grain size and grain number in rice. Theor Appl Genet 134, 3941–3950 (2021). https://doi.org/10.1007/s00122-021-03939-3

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