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Physical mapping of introgressed chromosome fragment carrying the fertility restoring (Rfo) gene for Ogura CMS in Brassica juncea L. Czern & Coss

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Abstract

Key message

Rfo is located on a radish chromosome fragment (~ 108 Kb), which is seated in the middle of a pretty large C genome translocation at the distal region of chromosome A09 of B. juncea.

Abstract

Ogura cytoplasmic male sterility (CMS) is used to produce hybrids in Indian mustard (Brassica juncea L.). Fertility restorers for this CMS were developed by cross-hybridizing B. juncea (AABB; 2n = 36) with B. napus (AACC; 2n = 38) carrying radish Rfo gene. This hybrid production system is normally stable, but many commercial mustard hybrids show male sterile contaminants. We aimed to identify linkage drag associated with Rfo by comparing hybridity levels of 295 handmade CMS x Rfo crosses. Although Rfo was stably inherited, hybridity was < 85 percent in several combinations. Genome re-sequencing of five fertility restorers, mapping sequencing reads to B. juncea reference and synteny analysis with Raphanus sativus D81Rfo genomic region (AJ550021.2) helped to detect ~ 108 Kb of radish chromosome (R) fragment substitution in all fertility restorers. This radish segment substitution was itself located amidst a large C genome translocation on the terminal region of chromosome A09 of B. juncea. The size of alien segment substitution varied from 11.3 (NTCN-R9) to 22.0 Mb (NAJR-102B-R). We also developed an in silico SSR map for chromosome A09 and identified many homoeologous A to the C genome exchanges in the introgressed region. A to the R genome exchanges were rare. Annotation of the substituted fragment showed the gain of many novel genes from R and C genomes and the loss of B. juncea genes from the corresponding region. We have developed a KASPar marker for marker-aided transfer of Rfo and testing hybridity levels in seed production lots.

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Acknowledgments

Researches were conducted with financial support from National Professor Project “Broadening the genetic base of Indian mustard (Brassica juncea) through alien introgressions and germplasm enhancement” awarded to SSB. Sequencing of fertility restorers was carried out with the financial support from Department of Biotechnology, Government of India, in the form of Centre of Excellence and Innovation in Biotechnology “Germplasm enhancement for crop architecture and defensive traits in Brassica juncea L. Czern. and Coss.”

Funding

This article was funded by Indian Council of Agricultural Research, Government of India (F.No.27(12)/2009-HRD-PartII) (grant number: ICAR45), Department of Biotechnology, Government of India (BT/01/CEIB/12/1/03).

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Author notes

  1. Santosh Gudi and Chhaya Atri have contributed equally to the work.

Authors and Affiliations

  1. Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, Punjab, India

    Santosh Gudi, Chhaya Atri, Anna Goyal, Navneet Kaur, Javed Akhtar, Meenakshi Mittal, Kawalpreet Kaur, Gurpreet Kaur & Surinder S. Banga

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  1. Santosh Gudi
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  2. Chhaya Atri
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  3. Anna Goyal
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  7. Kawalpreet Kaur
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  9. Surinder S. Banga
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Contributions

SG conducted field experiments. SSB and CA developed the fertility restorers and analyzed field data. JA, NK and SG carried out the bioinformatic data analysis. AG assisted in field and laboratory studies. MM helped with annotation. GK developed KASPar marker. CA and SG wrote the manuscript. SSB planned the experiments, supervised the study and edited the manuscript.

Corresponding author

Correspondence to Surinder S. Banga.

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Communicated by Carlos F. Quiros.

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Gudi, S., Atri, C., Goyal, A. et al. Physical mapping of introgressed chromosome fragment carrying the fertility restoring (Rfo) gene for Ogura CMS in Brassica juncea L. Czern & Coss. Theor Appl Genet 133, 2949–2959 (2020). https://doi.org/10.1007/s00122-020-03648-3

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