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Characterization of a radish introgression carrying the Ogura fertility restorer gene Rfo in rapeseed, using the Arabidopsis genome sequence and radish genetic mapping

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Abstract

The radish Rfo gene restores male fertility in radish or rapeseed plants carrying Ogura cytoplasmic male-sterility. This system was first discovered in radish and was transferred to rapeseed for the production of F1 hybrid seeds. We aimed to identify the region of the Arabidopsis genome syntenic to the Rfo locus and to characterize the radish introgression in restored rapeseed. We used two methods: amplified consensus genetic markers (ACGMs) in restored rapeseed plants and construction of a precise genetic map around the Rfo gene in a segregating radish population. The use of ACGMs made it possible to detect radish orthologs of Arabidopsis genes in the restored rapeseed genome. We identified radish genes, linked to Rfo in rapeseed and whose orthologs in Arabidopsis are carried by chromosomes 1, 4 and 5. This indicates several breaks in colinearity between radish and Arabidopsis genomes in this region. We determined the positions of markers relative to each other and to the Rfo gene, using the progeny of a rapeseed plant with unstable meiotic transmission of the radish introgression. This enabled us to produce a schematic diagram of the radish introgression in rapeseed. Markers which could be mapped both on radish and restored rapeseed indicate that at least 50 cM of the radish genome is integrated in restored rapeseed. Using markers closely linked to the Rfo gene in rapeseed and radish, we identified a contig spanning six bacterial artificial chromosome (BAC) clones on Arabidopsis chromosome 1, which is likely to carry the orthologous Rfo gene.

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Acknowledgements

This work was supported by Génoplante, the French Consortium for Plant Genomics. We would like to thank R. Horvais for producing the radish mapping populations. We greatly appreciated support from, and stimulating discussions with, D. Brunel during experimental work and the writing of the manuscript. We would like to thank A. Bérard, M. Caffé, N. Froger and S. Perrinet for technical support, and M. Fourmann for kindly providing us with consensus primers for some target genes. We would also like to thank R. Berthomé, S. Bonhomme, M. Grelon, H. McKhann, C. Mézard and H. Mireau for critical reading of the manuscript, and I. Small for stimulating discussions. We thank P. Guerche for constant support. The work was carried out in compliance with the current laws governing research and development programs in France.

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Authors and Affiliations

  1. Station de Génétique et d'Amélioration des Plantes, INRA, Route de Saint-Cyr, 78026, Versailles cedex, France

    Sandra Giancola, Georges Pelletier & Françoise Budar

  2. UMR INRA-ENSAR, Amélioration des Plantes et Biotechnologies Végétales, BP 35327, 35653, Le Rheu Cedex, France

    Sylvie Marhadour, Vanessa Clouet, Hélène Falentin-Guyomarc'h, Cyril Falentin, Michel Renard & Régine Delourme

  3. Unité de Recherches en Génomique Végétale INRA, 2 rue Gaston Crémieux/CP5708, 91057, Evry cedex, France

    Sophie Desloire, Wassila Laloui & Abdelhafid Bendahmane

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  1. Sandra Giancola
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  2. Sylvie Marhadour
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  10. Abdelhafid Bendahmane
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  11. Régine Delourme
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Correspondence to Françoise Budar.

Additional information

Communicated by H. C. Becker

S. Giancola and S. Marhadour contributed equally to this work

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Giancola, S., Marhadour, S., Desloire, S. et al. Characterization of a radish introgression carrying the Ogura fertility restorer gene Rfo in rapeseed, using the Arabidopsis genome sequence and radish genetic mapping. Theor Appl Genet 107, 1442–1451 (2003). https://doi.org/10.1007/s00122-003-1381-2

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