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TaXA21-A1 on chromosome 5AL is associated with resistance to multiple pests in wheat

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Abstract

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The wheat ortholog of the rice gene OsXA21 against bacterial leaf blight showed resistance to multiple pests in bread wheat but different interacting proteins.

Abstract

A quantitative trait locus QYr.osu-5A on the long arm of chromosome 5A in bread wheat (Triticum aestivum L., 2n = 6x = 42; AABBDD) was previously reported to confer consistent resistance in adult plants to predominant stripe rust races, but the gene causing the quantitative trait locus (QTL) is not known. Single-nucleotide polymorphism (SNP) markers were used to saturate the QTL region. Comparative and syntenic regions between wheat and rice (Oryza sativa) were applied to identify candidate genes for QYr.osu-5A. TaXA21-A1, which is referred to as a wheat ortholog of OsXA21-like gene on chromosome 9 in rice, was mapped under the peak of the QYr.osu-5A. TaXA21-A1 not only explained the phenotypic variation in reaction to different stripe rust races but also showed significant effects on resistance to powdery mildew and Hessian fly biotype BP. The natural allelic variation resulted in the alternations of four amino acids in deduced TaXA21-A1 proteins. The interacting proteins of TaXA21-A1 were different from those identified by OsXA21 on rice chromosome 11 against bacterial leaf blight. TaXA21-A1 confers unique resistance against multiple pests in wheat but might not have common protein interactors or thus overlapping functions with OsXA21 in rice. XA21 function has diverged during evolution of cereal crops. The molecular marker developed for TaXA21-A1 would accelerate its application of the candidate gene at the QYr.osu-5A locus in wheat breeding programs.

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Acknowledgments

This work was supported by USDA-NIFA T-CAP grant No. 2011-68002-30029, and the Oklahoma Wheat Research Foundation.

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    Authors and Affiliations

    1. Department of Plant and Soil Sciences, Oklahoma State University, Stillwater, OK, 74074, USA

      Meiyan Liu, Lei Lei, Carol Powers, Brett F. Carver & Liuling Yan

    2. School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, China

      Meiyan Liu

    3. Department of Plant Genetics and Breeding, China Agricultural University, Beijing, 100193, China

      Zhiyong Liu

    4. Wheat Genetics, Quality, Physiology, and Disease Research Unit, USDA-ARS, Pullman, WA, 99164, USA

      Kimberly G. Campbell & Xianming Chen

    5. Department of Crop and Soil Sciences, Washington State University, Pullman, WA, 99164, USA

      Kimberly G. Campbell

    6. Department of Plant Pathology, Washington State University, Pullman, WA, 99164, USA

      Xianming Chen

    7. Hard Winter Wheat Genetics Research Unit, USDA-ARS, Manhattan, KS, 66506, USA

      Robert L. Bowden

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    1. Meiyan Liu
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    Communicated by X. Qi.

    M. Liu and L. Lei equally contributed to the paper.

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    Multiple sequence alignment of XA21 proteins. (PDF 325 kb)

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    Liu, M., Lei, L., Powers, C. et al. TaXA21-A1 on chromosome 5AL is associated with resistance to multiple pests in wheat. Theor Appl Genet 129, 345–355 (2016). https://doi.org/10.1007/s00122-015-2631-9

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