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High-resolution analysis of a QTL for resistance to Stagonospora nodorum glume blotch in wheat reveals presence of two distinct resistance loci in the target interval

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Abstract

Stagonospora nodorum glume blotch (SNG), caused by the necrotrophic fungus Stagonospora nodorum, is one of the economically important diseases of bread wheat (Triticum aestivum L.). Resistance to SNG is known to be quantitative and previous studies of a recombinant inbred line (RIL) population identified a major quantitative trait locus (QTL) for resistance to SNG on the short arm of chromosome 3B. To localize this QTL (QSng.sfr-3BS) with high resolution, we constructed a genetic map for the QTL target region using information from sequenced flow-sorted chromosomes 3B of the two parental cultivars ‘Arina’ and ‘Forno’, the physical map of chromosome 3B of cultivar ‘Chinese Spring’ and BAC-clone sequences. The mapping population of near-isogenic lines (NIL) was evaluated for SNG resistance in field infection tests. NILs segregated for disease resistance as well as for plant height; additionally, we observed a high environmental influence on the trait. Our analysis detected a strong negative correlation of SNG resistance and plant height. Further analysis of the target region identified two linked loci associated with SNG resistance. One of them was also associated with plant height, revealing an effect of QSng.sfr-3BS on plant height that was hidden in the RIL population. This result demonstrates an unexpectedly high genetic complexity of resistance controlled by QSng.sfr-3BS and shows the importance of the study of QTL in mendelized form in NILs.

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Acknowledgments

We thank Bea Senger and Stefan Kellenberger for excellent technical assistance with the field experiments. We thank Philip Streckeisen for the guidance on SNB field scorings. We also thank Bea Senger for the technical help with the plant material in the greenhouse. We thank Hana Simkova and Jaroslav Dolezel for isolation of chromosome 3B by flow cytometry. We also thank Matthias Helling for the help with the statistical analysis. The research leading to these results received funding from the European Community’s Seventh Framework Programme (FP7/2007-2013) under the grant agreement FP7-212019 (Triticeae Genome) and was supported in the framework of the European Cooperation in Science and Technology FA0604 (Tritigen). This work was also supported by a grant from the Swiss National Science Foundation 31003A_127061. The experiments in this study comply with the current laws of Switzerland.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, 8008, Zurich, Switzerland

    Margarita Shatalina, Thomas Wicker & Beat Keller

  2. Research Institute of Organic Agriculture, 5070, Frick, Switzerland

    Monika Messmer

  3. INRA, Genetics, Diversity and Ecophysiology of Cereals, 63100, Clermont Ferrand, France

    Catherine Feuillet, Etienne Paux & Frédéric Choulet

  4. Research Station Agroscope Changins-Wädenswil ACW, 1260, Nyon, Switzerland

    Fabio Mascher

Authors
  1. Margarita Shatalina
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  2. Monika Messmer
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  3. Catherine Feuillet
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  4. Fabio Mascher
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  5. Etienne Paux
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  6. Frédéric Choulet
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Correspondence to Beat Keller.

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Communicated by S. Dreisigacker.

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Shatalina, M., Messmer, M., Feuillet, C. et al. High-resolution analysis of a QTL for resistance to Stagonospora nodorum glume blotch in wheat reveals presence of two distinct resistance loci in the target interval. Theor Appl Genet 127, 573–586 (2014). https://doi.org/10.1007/s00122-013-2240-4

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