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High macro-collinearity between lima bean (Phaseolus lunatus L.) and the common bean (P. vulgaris L.) as revealed by comparative cytogenetic mapping

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Abstract

Common bean (P. vulgaris) and lima bean (P. lunatus) are the most important crop species from the genus Phaseolus. Both species have the same chromosome number (2n = 22) and previous cytogenetic mapping of BAC clones suggested conserved synteny. Nevertheless, karyotype differences were observed, suggesting structural rearrangements. In this study, comparative cytogenetic maps for chromosomes 3, 4 and 7 were built and the collinearity between the common bean and lima bean chromosomes was investigated. Thirty-two markers (30 BACs and 2 bacteriophages) from P. vulgaris were hybridized in situ on mitotic chromosomes from P. lunatus. Nine BACs revealed a repetitive DNA pattern with pericentromeric distribution and 23 markers showed unique signals. Nine of these markers were mapped on chromosome 3, eight on chromosome 4 and six on chromosome 7. The order and position of all analyzed BACs were similar between the two species, indicating a high level of macro-collinearity. Thus, although few inversions have probably altered centromere position in other chromosomes, the main karyotypic differences were associated with the repetitive DNA fraction.

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Acknowledgments

We thank Heloisa Torres (EMBRAPA Arroz e Feijão) for providing the seeds, Dr. Paul Gepts (University of California) for the BAC clones and Dr. Valérie Geffroy (Université Paris Sud) for the bacteriophage clones. C. A. and A. P.-H. were supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil. The work was supported by CNPq, Brazil.

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  1. Cícero Almeida

    Present address: Laboratory of Genetics Resources, Campus Arapiraca, Universidade Federal de Alagoas, Arapiraca, AL, 57360-910, Brazil

Authors and Affiliations

  1. Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Universidade Federal de Pernambuco, Recife, PE, 50670-420, Brazil

    Cícero Almeida & Andrea Pedrosa-Harand

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  1. Cícero Almeida
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  2. Andrea Pedrosa-Harand
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Correspondence to Andrea Pedrosa-Harand.

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Communicated by B. Friebe.

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Almeida, C., Pedrosa-Harand, A. High macro-collinearity between lima bean (Phaseolus lunatus L.) and the common bean (P. vulgaris L.) as revealed by comparative cytogenetic mapping. Theor Appl Genet 126, 1909–1916 (2013). https://doi.org/10.1007/s00122-013-2106-9

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