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Evolution of chromosome 6 of Solanum species revealed by comparative fluorescence in situ hybridization mapping

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Abstract

Comparative genetic linkage mapping using a common set of DNA markers in related species is an important methodology in plant genome research. Here, we demonstrate a comparative fluorescence in situ hybridization (FISH) mapping strategy in plants. A set of 13 bacterial artificial chromosome clones spanning the entire length of potato chromosome 6 was used for pachytene chromosome-based FISH mapping in seven distantly related Solanum species including potato, tomato, and eggplant. We discovered one paracentric inversion and one pericentric inversion within specific lineages of these species. The comparative FISH mapping data revealed the ancestral structure of this chromosome. We demonstrate that comparative FISH mapping is an efficient and powerful methodology to study chromosomal evolution among plant species diverged for up to 12 million years.

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Acknowledgments

This work was supported by grant DBI-0604907 from the National Science Foundation. Q.F.L. was supported by a fellowship from the Ministry of Education, the People’s Republic of China. D.M.S. was supported by the USDA and by NSF DEB 0316614 and USDA National Research Initiative Grant 2008-35300-18669. M.I. was on leave of absence from CNR—Institute of Plant Genetics, Via Amendola 165/A, 70126 Bari, Italy.

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Authors and Affiliations

  1. Department of Horticulture, University of Wisconsin—Madison, Madison, WI, 53706, USA

    Qunfeng Lou, Marina Iovene & Jiming Jiang

  2. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, People’s Republic of China

    Qunfeng Lou

  3. U.S. Department of Agriculture—Agricultural Research Service, Vegetable Crops Unit, Department of Horticulture, University of Wisconsin, Madison, WI, 53706, USA

    David M. Spooner

  4. Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA

    C. Robin Buell

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  1. Qunfeng Lou
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  2. Marina Iovene
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  3. David M. Spooner
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  4. C. Robin Buell
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  5. Jiming Jiang
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Correspondence to Jiming Jiang.

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Communicated by P. Shaw

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Lou, Q., Iovene, M., Spooner, D.M. et al. Evolution of chromosome 6 of Solanum species revealed by comparative fluorescence in situ hybridization mapping. Chromosoma 119, 435–442 (2010). https://doi.org/10.1007/s00412-010-0269-6

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