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Conditional QTL underlying resistance to late blight in a diploid potato population

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Abstract

A large number of quantitative trait loci (QTL) for resistance to late blight of potato have been reported with a “conventional” method in which each phenotypic trait reflects the cumulative genetic effects for the duration of the disease process. However, as genes controlling response to disease may have unique contributions with specific temporal features, it is important to consider the phenotype as dynamic. Here, using the net genetic effects evidenced at consecutive time points during disease development, we report the first conditional mapping of QTL underlying late blight resistance in potato under five environments in Peru. Six conditional QTL were mapped, one each on chromosome 2, 7 and 12 and three on chromosome 9. These QTL represent distinct contributions to the phenotypic variation at different stages of disease development. By comparison, when conventional mapping was conducted, only one QTL was detected on chromosome 9. This QTL was the same as one of the conditional QTL. The results imply that conditional QTL reflect genes that function at particular stages during the host–pathogen interaction. The dynamics revealed by conditional QTL mapping could contribute to the understanding of the molecular mechanism of late blight resistance and these QTL could be used to target genes for marker development or manipulation to improve resistance.

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Acknowledgments

This work was partially funded by the Natural Science Foundation of Hubei Province of China (2010CDA102), the “948” Project from the Ministry of Agriculture of China [2006-G12(B)]and the Earmarked Fund for Modern Agro-industry Technology Research System of China(nycytx-15). Research at CIP was funded by Technical Coordination Secretariat of the Consultative Group on International Agricultural Research (Peru) and United States Agency for International Development (USAID). We are thankful to Luis Rivera for running SSR marker analysis at CIP.

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Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology (Huazhong Agricultural University), Ministry of Education, National Center for Vegetable Improvement (Central China), Potato Engineering and Technology Research Center of Hubei Province, Huazhong Agricultural University, Wuhan, Hubei, 430070, People’s Republic of China

    Jingcai Li, Zhendong Tian, Jun Liu, Botao Song & Conghua Xie

  2. International Potato Center, Lima 12, P.O. Box 1558, Lima, Peru

    Hannele Lindqvist-Kreuze, Juan Landeo, Leticia Portal, Manuel Gastelo, Julio Frisancho, Laura Sanchez, Dennis Meijer & Merideth Bonierbale

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  1. Jingcai Li
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  2. Hannele Lindqvist-Kreuze
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  3. Zhendong Tian
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  4. Jun Liu
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  12. Conghua Xie
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  13. Merideth Bonierbale
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Correspondence to Hannele Lindqvist-Kreuze or Conghua Xie.

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Communicated by C. Gebhardt.

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Li, J., Lindqvist-Kreuze, H., Tian, Z. et al. Conditional QTL underlying resistance to late blight in a diploid potato population. Theor Appl Genet 124, 1339–1350 (2012). https://doi.org/10.1007/s00122-012-1791-0

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