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QTL underlying the resistance to soybean aphid (Aphis glycines Matsumura) through isoflavone-mediated antibiosis in soybean cultivar ‘Zhongdou 27’

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Abstract

Soybean aphid (Aphis glycines Matsumura) results in severe yield loss of soybean in many soybean-growing countries of the world. A few loci have been previously identified to be associated with the aphid resistance in soybean. However, none of them was via isoflavone-mediated antibiosis process. The aim of the present study was to conduct genetic analysis of aphid resistance and to identify quantitative trait loci (QTL) underlying aphid resistance in a Chinese soybean cultivar with high isoflavone content. One hundred and thirty F5:6 derived recombinant inbred lines from the ‘Zhongdou 27’ × ‘Jiunong 20’ cross were used. Two QTL were directly associated with resistance to aphid as measured by aphid damage index. qRa_1, close to Satt470 on soybean linkage group (LG) A2 (chromosome 8), was consistently detected for 3- and 4-week ratings and explained a large portion of phenotypic variations ranging from 25 to 35%. qRa_2, close to Satt144 of LG F (chromosome 13), was detected for 3- and 4-week ratings and could explain 7 and 11% of the phenotypic variation, respectively. These two QTL were highly associated with high isoflavone content and both positive alleles were derived from ‘Zhongdou 27’, a cultivar with higher isoflavone content. The results revealed that higher individual or total isoflavones contents in soybean lines could protect soybean against aphid attack. These two QTL detected jointly provide potential for marker-assisted selection to improve the resistance of soybean cultivars to aphid along with the increase of isoflavone content.

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Acknowledgments

This study was conducted in the Key Laboratory of Soybean Biology of Chinese Education Ministry and Soybean Development Centre of Agricultural Ministry, and financially supported by National High Technology Projects (2006AA10Z1F1 and 2006AA100104-4), National Nature Science Foundation Projects (60932008, 30971810), National 973 Project (2009CB118400) and Provincial Education Ministry for the Team of Soybean Molecular Design.

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Authors and Affiliations

  1. Soybean Research Institute, Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin, 150030, China

    Fanli Meng, Yingpeng Han, Weili Teng, Yongguang Li & Wenbin Li

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  1. Fanli Meng
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  2. Yingpeng Han
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  3. Weili Teng
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  4. Yongguang Li
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  5. Wenbin Li
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Correspondence to Wenbin Li.

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Communicated by I. Rajcan.

F. Meng and Y. Han contributed equally to this work.

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Meng, F., Han, Y., Teng, W. et al. QTL underlying the resistance to soybean aphid (Aphis glycines Matsumura) through isoflavone-mediated antibiosis in soybean cultivar ‘Zhongdou 27’. Theor Appl Genet 123, 1459–1465 (2011). https://doi.org/10.1007/s00122-011-1680-y

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