Abstract
The ability to learn food odors inside the nest and to associate them with food sources in the field is of essential importance for the recruitment of nestmates in social bees. We investigated odor learning by workers within the hive and the influence of these odors on their food choice in the field in the stingless bee Scaptotrigona pectoralis. During the experiments, recruited bees had to choose between two feeders, one with an odor that was present inside the nest during the recruitment process, and one with an unknown odor. In all experiments with different odor combinations (linalool/phenylacetaldehyde, geraniol/eugenol) a significant majority of bees visited the feeder with the odor they had experienced in their nest (χ 2-tests; p < 0.05). By contrast, the bees showed no preference for one of two feeders when they were either baited with the same odor (linalool) or contained no odor. Our results clearly show that naïve workers of S. pectoralis can learn the odor of a food source during the recruitment process from the nest atmosphere and that their subsequent food search in the field is influenced by the learned odor.
References
Aguilar I (2004) Communication and recruitment for the collection of food in stingless bees: a behavioral approach. Dissertation, University of Utrecht
Aguilar I, Fonseca A, Biesmeijer JC (2005) Recruitment and communication of food source location in three species of stingless bees (Hymenoptera, Apidae, Meliponini). Apidologie 36:313–324
Arenas A, Fernández VM, Farina WM (2007) Floral odor learning within the hive affects honeybees´ foraging decisions. Naturwissenschaften 94:218–222
Barth FG (1991) Insects and flowers. The biology of a partnership. Princeton University Press, Princeton
Barth FG, Hrncir M, Jarau S (2008) Signals and cues in the recruitment behaviour of stingless bees (Meliponini). J Comp Physiol A 194:313–327
Dalla Torre CG de (1896) Catalogus Hymenopterorum; Hucusque Descriptorum Systematicus et Synonymicus. Leipzig: Engelmann Vol. 10 viii+1–643 pp
Dornhaus A, Chittka L (1999) Evolutionary origins of bee dances. Nature 401:38
Dornhaus A, Chittka L (2004) Information flow and regulation of foraging activity in bumble bees (Bombus spp.). Apidologie 35:183–192
Farina W, Grüter C (2009) Trophallaxis. A mechanism of information transfer. In: Jarau S, Hrncir M (eds) Food exploitation by social insects. Ecological, behavioral, and theoretical approaches. CRC, Boca Raton, pp 183–197
Farina WM, Grüter C, Acosta L, Mc Cabe S (2007) Honeybees learn floral odors while receiving nectar from foragers within the hive. Naturwissenschaften 94:55–60
Gil M, De Marco RJ (2005) Olfactory learning by means of trophallaxis in Apis mellifera. J Exp Biol 208:671–680
Grüter C, Acosta LE, Farina WM (2006) Propagation of olfactory information within the honeybee hive. Behav Ecol Sociobiol 60:707–715
Hrncir M (2009) Mobilizing the foraging force. Mechanical signals in stingless bee recruitment. In: Jarau S, Hrncir M (eds) Food exploitation by social insects. Ecological, behavioral, and theoretical approaches. CRC, Boca Raton, pp 199–221
Jarau S (2009) Chemical communication during food exploitation in stingless bees. In: Jarau S, Hrncir M (eds) Food exploitation by social insects. Ecological, behavioral, and theoretical approaches. CRC, Boca Raton, pp 223–249
Jarau S, Hrncir M, Zucchi R, Barth FG (2000) Recruitment behaviour in stingless bees. Melipona scutellaris and M. quadrifasciata. I. Foraging at food sources differing in direction and distance. Apidologie 31:81–91
Jarau S, Hrncir M, Schmidt VM, Zucchi R, Barth FG (2003) Effectiveness of recruitment behavior in stingless bees (Apidae, Meliponini). Insect Soc 50:365–374
Kerr WE (1969) Some aspects of the evolution of social bees (Apidae). Evol Biol 3:119–175
Kerr WE, Ferreira A, de Mattos NS (1963) Communication among stingless bees—additional data (Hymenoptera: Apidae). J NY Entomol Soc 71:80–90
Knudsen JT, Tollsten L, Berström LG (1993) Floral scents—a checklist of volatile compounds isolated by head-space techniques. Phytochemistry 33:253–280
Lindauer M (1956) Über die Verständigung bei indischen Bienen. Z Vergl Physiol 38:521–557
Lindauer M, Kerr WE (1958) Die gegenseitige Verständigung bei den stachellosen Bienen. Z Vergl Physiol 41:405–434
Lindauer M, Kerr WE (1960) Communication between the workers of stingless bees. Bee World 41:29-41–65-71
Mc Cabe SI, Farina WM (2009) Odor information transfer in the stingless bee Melipona quadrifasciata: effect of in-hive experiences on classical conditioning of proboscis extension. J Comp Physiol A 195:113–122
Mc Cabe SI, Hartfelder K, Santana WC, Farina WM (2007) Odor discrimination in classical conditioning of proboscis extension in two stingless bee species in comparison to Africanized honeybees. J Comp Physiol A 193:1089–1099
Menzel R (1999) Memory dynamics in the honeybee. J Comp Physiol A 185:323–340
Menzel R, Müller U (1996) Learning and memory in honeybees: from behavior to neural substrates. Annu Rev Neurosci 19:379–404
Michener CD (2000) The bees of the world. The John Hopkins University Press, Baltimore
Molet M, Chittka L, Raine NE (2009) How floral odours are learned inside the bumblebee (Bombus terrestris) nest. Naturwissenschaften 96:213–219
Nieh JC (2004) Recruitment communication in stingless bees (Hymenoptera, Apidae, Meliponini). Apidologie 35:159–182
Praz CJ, Müller A, Dorn S (2008) Host recognition in a pollen-specialist bee: evidence for a genetic basis. Apidologie 39:547–557
Raine NE, Chittka L (2009) Measuring the adaptiveness of social insect foraging strategies. An empirical approach. In: Jarau S, Hrncir M (eds) Food exploitation by social insects. Ecological, behavioral, and theoretical approaches. CRC, Boca Raton, pp 9–28
Reinhard J, Srinivasan MV (2009) The role of scents in honey bee foraging and recruitment. In: Jarau S, Hrncir M (eds) Food exploitation by social insects. Ecological, behavioral, and theoretical approaches. CRC, Boca Raton, pp 165–182
Sánchez D, Kraus FB, de Jesús Hernández M, Vandame R (2007) Experience, but not distance, influences the recruitment precision in the stingless bee Scaptotrigona mexicana. Naturwissenschaften 94:567–573
Schmidt VM, Zucchi R, Barth FG (2003) A stingless bee marks the feeding site in addition to the scent path (Scaptotrigona aff. depilis). Apidologie 34:237–248
Slaa EJ, Hughes WHO (2009) Local enhancement, local inhibition, eavesdropping, and the parasitism of social insect communication. In: Jarau S, Hrncir M (eds) Food exploitation by social insects. Ecological, behavioral, and theoretical approaches. CRC, Boca Raton, pp 147–164
Toda NRT, Song J, Nieh JC (2009) Bumblebees exhibit the memory spacing effect. Naturwissenschaften 96(10): 1185–1191
von Frisch K (1965) Tanzsprache und Orientierung der Bienen. Springer, Berlin
Wilson EO (1971) The insect societies. Belknap Press of Harvard University Press, Cambridge
Acknowledgments
We would like to express our thanks to Javier Guevara from the Ministerio del Ambiente y Energia (MINAE) of Costa Rica, for his help in obtaining research permits (080-2006-SINAC, 133-2007-SINAC). This work was supported by a scholarship from the German Academic Exchange Service (DAAD) granted to CR.
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Reichle, C., Jarau, S., Aguilar, I. et al. Recruits of the stingless bee Scaptotrigona pectoralis learn food odors from the nest atmosphere. Naturwissenschaften 97, 519–524 (2010). https://doi.org/10.1007/s00114-010-0662-2
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DOI: https://doi.org/10.1007/s00114-010-0662-2