Abstract
Introduction
Focal adhesion kinase (FAK) is a nonreceptor tyrosine kinase protein frequently overexpressed in cancer and has been linked to an increase in the stem cell population of tumors, resistance to therapy, and metastatic spread. Pharmacological FAK inhibition in pancreatic cancer has received increased attention over the last few years, either alone or in combination with other therapeutics including chemotherapy and immunotherapy. However, its prognostic value and its role in radioresistance of pancreatic ducal adenocarcinoma (PDAC) is unknown.
Methods and materials
Using the TCGA and GTEx databases, we investigated the genetic alterations and mRNA expression levels of PTK2 (the encoding-gene for FAK) in normal pancreatic tissue and pancreatic cancer and its impact on patient survival. Furthermore, we evaluated the expression of FAK and its tyrosine domain Ty-397 in three pancreatic cancer cell lines. We went further and evaluated the role of a commercial FAK tyrosine kinase inhibitor VS-4718 on the viability and radiosensitization of the pancreatic cell lines as well as its effect on the extracellular matrix (ECM) production from the pancreatic stellate cells. Furthermore, we tested the effect of combining radiation with VS-4718 in a three-dimensional (3D) multicellular pancreatic tumor spheroid model.
Results
A database analysis revealed a relevant increase in genetic alterations and mRNA expression of the PTK2 in PDAC, which were associated with lower progression-free survival. In vitro, there was only variation in the basal phosphorylation level of FAK in cell lines. VS-4718 radiosensitized pancreatic cell lines only in the presence of ECM-producing pancreatic stellate cells and markedly reduced the ECM production in the stromal cells. Finally, using a 3D multicellular tumor model, the combination of VS-4718 and radiotherapy significantly reduced the growth of tumor aggregates.
Conclusion
Pharmacological inhibition of FAK in pancreatic cancer could be a novel therapeutic strategy as our results show a radiosensitization effect of VS-4718 in vitro in a multicellular 2D- and in a 3D-model of pancreatic cancer.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
With a 5-year overall survival of less than 5%, pancreatic ductal adenocarcinoma is the 4th leading cause of cancer-related mortality in the western world and is expected to remain a leading cause of cancer-related mortality within the next decade due to the increasing incidence [1, 2]. Despite the grim prognosis, the relative pace of discovering novel treatments has been much slower than those for many other types of cancer.
Several reasons have been proposed to explain the poor prognosis. These include lack of specific symptoms for early disease diagnosis, the complexity of the molecular survival mechanisms, and the desmoplastic reaction (DR), which protects cancer cells against the therapy and facilitates their invasion into neighboring structures [3,4,5].
Clinically, pancreatic ducal adenocarcinoma (PDAC) is categorized into four stages: resectable, borderline-resectable, locally advanced and metastatic, based on the infiltration of the surrounding vascular structures and distant metastases [6]. While radiation therapy was shown to have little if any role in the first and last stages, the question of a potential benefit from radiotherapy in the borderline-resectable and locally advanced forms of the disease remains to be answered [7, 8]. Clinical studies have demonstrated a positive impact of fractionated chemoradiation [9] and stereotactic body radiotherapy (SBRT) [10] in advanced PDAC. However, it has been suggested that dose escalation is essential for this impact [11, 12]. Other studies did not conclude a benefit from adding chemoradiation in those stages [13]. Such uncertainty about the role of radiation therapy in PDAC could be attributed to the poor understanding of the molecular complexity of radioresistance mechanisms in pancreatic cancer. The interest in molecular targeted therapies in PDAC continues to increase. Here, we investigated the potential role of the focal adhesion kinase (FAK) in improving the radiosensitivity of PDAC.
FAK is a scaffolding nonreceptor tyrosine kinase, encoded by the PTK2 gene (8q24.3). FAK is known to be involved in various functions within the cell, including cell–extracellular matrix (ECM) interactions [14], motility, anchorage-independent growth, migration [15, 16], proliferation, survival, and apoptosis [17], and expressed within the cancer stem cell (CSC) pool [18]. Structurally, it comprises three domains: an N‑terminal FERM (protein 4.1, ezrin, radixin, moesin) domain, a central kinase domain, and a c-terminal region containing proline-rich motifs and FAT (focal adhesion targeting). During activation, FAK undergoes conformational changes that result in the release of the bond between FERM and the kinase domains resulting in autophosphorylation of the Tyr397. Full activation of FAK occurs by the binding of Src to Tyr397 and the phosphorylation of other tyrosine domains within the activation loop (Tyr576 and 577) [19].
In the current study, we demonstrated alterations in the FAK encoding gene, PTK2, and mRNA expression using cBioPortal and the impact of those alterations on the prognosis. We then tested the effect of FAK inhibition in vitro on proliferation and the alteration of radiation sensitivity of cancer cell lines alone and in presence of stellate cells, using a PTK2 siRNA and a commercially available potent FAK-tyrosine kinase inhibitor, VS-4718.
VS-4718 is an oral, selective FAK tyrosine kinase inhibitor (TKI) previously known as PND-1186. It has been tested in vitro and in vivo, and shows inhibition of tumor growth and spreading, selective depletion of the CSC pool and was found to potentiate the effect of other conventional chemotherapeutics such as paclitaxel and cisplatin [20,21,22]. To our knowledge, VS-4718 has not been tested until now for the radiosensitization of pancreatic cancer cells.
Materials and methods
PTK2 gene alterations and expression analysis
PTK2 gene alterations and mRNA in PDAC patients were queried online on cBioPortal for Cancer Genomics (http://www.cbioportal.org/) [23, 24] using the pancreatic adenocarcinoma (TCGA, PanCancer Atlas cohort) dataset. We queried mutations, putative copy-number alterations, and mRNA expressions (RNA Seq V2 RSEM with z‑scores = ±2) and survival analysis for the PTK2 gene. We exported the data from the Oncoprint and survival screens. Differential expression of PTK2 between TCGA dataset and normal pancreatic tissue from Genotype-Tissue Expression (GTEx) [25] was queried using the GEPIA server [26]. PTK2 expression in cancer cell lines was queried in the GEMicCCL portal [28].
Cell lines and reagents
Human PDAC cell lines (PCC): Panc‑1, MIA PaCa 2 (American Type Culture Collection, Manassas, VA, USA) and PSN‑1 (Merck & Co., Inc., West Point, PA, USA). Human pancreatic stellate cell lines (h.PSCs) were kindly provided from Dr. J. Kleef’s Laboratory (Halle University, Germany), LTC-14 (immortalized rat pancreatic stellate cells) [29] were kindly provided by Dr. G. Sparmann (Rostock, Germany). Panc‑1, PSN‑1 and LTC-14 were grown in culture with DMEM and MIA PaCa 2 with RPMI 1640 + 10% fetal bovine serum (FBS) + 1% penicillin and streptomycin. Human PSCs were cultured in stellate cell medium: MDEM/F-12 and DMEM low glucose medium (1:1) + 16% FBS + 1% penicillin and streptomycin.
VS-4718 (Selleck Chemicals, Houston, TX, USA) was dissolved in dimethyl sulfoxide (DMSO); vehicle controls were equal volumes of the same concentration of DMSO.
Western blotting
The antibodies used in the study: FAK antibody #3285, p‑FAK (Tyr397) antibody #3283 both at 1:1000 dilution (cell signaling, Cell Signaling Technology, Danvers, MA, USA), GAPDH and β‑actin at 1:10,000 dilution (Sigma-Aldrich, St. Louis, MI, USA). Cell lysate, protein separation, and immunoblotting were carried out as previously described [30].
siRNA transfection
Cells were transfected according to the manufacturer’s instructions using the PTK2-siGENOME SMARTpool (Dharmacon, Lafayette, CO, USA) a mixture of four siRNA with the following target sequences:
-
GCGAUUAUAUGUUAGAGAU
-
GGGCAUCAUUCAGAAGAUA
-
UAGUACAGCUCUUGCAUAU
-
GGACAUUAUUGGCCACUGU
or ON-TARGET plus—nontargeting siRNA #2 (Dharmacon, Lafayette, CO, USA). Cells were incubated for 48 h with the transfection mixture and then used in experiments and to obtain cell lysate for Western blots.
Cell viability assay
Cell viability and cytotoxicity were estimated using the MTT assay (3‑[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) (Sigma-Aldrich, St. Louis, MI, USA). Cell lines (Panc‑1, PSN‑1, MIA PaCa 2 and LTC-14) were seeded in 96-well plates at a density between 1–5 × 104 cells/well according to each cell line, and 24 h after incubation, the cells were treated with VS-4718 from 0–5 μM in the medium for 24 and 48 h. Cells were incubated with equal amounts of MTT (5 mg/ml) for 4 h in the dark at 37 °C. The supernatants were carefully discarded and replaced by 100 μl DMSO to dissolve the MTT in the cells. Finally, the optical density (OD) of each well was measured using an ELISA microplate reader at a wavelength of 570 nm. The results of each dose were normalized to the control group to calculate the viable fraction; each concentration was tested in triplicate and the mean was used and each experiment was repeated three times. The cytotoxicity of VS-4718 was expressed here as IC50 (which was estimated from a linear regression analysis of the experimental data).
Clonogenic survival assay
Cancer cell lines were seeded directly into 6‑well plates as triplicates in monoculture settings. In the coculture assay, LTC-14 cells were seeded simultaneously with PCCs as they rapidly proliferate and adhere to the plates or hPSCs were seeded overnight before the PCCs to ensure they were adherent in the 6‑well plates with ratio 1:2–4 (PCCs: LTC14/hPSCs). For clonogenic survival assay on top of collagen I, 6‑well plates were coated with collagen G (Biochrom Inc., Germany).
Either VS-4718 (2.5 µM) or vehicle was added to the culture medium, then the 6 well-plates were incubated for 2 h and finally were irradiated using Cs-137, Gammacell 40 Exactor (Best Theratronics, Canada).
The culture medium was replaced 22 h after irradiation to wash out the treatment and plates were incubated at 37 °C and 5% CO2 for 10–14 days then fixed and stained using crystal violet.
Each experiment was repeated three times and results were plotted on a survival curve as mean ± standard deviation.
Immunofluorescence labeling of γ-H2AX foci and cell cycle analysis
Three cell lines were co-cultured with PSCs as duplicates in two 96-well microplates, 190 µm Clear Bottom (Greiner Bio-one, Germany) and incubated together overnight at 37 °C to allow cells to attain growth until the mid-log phase before drug treatment. Cells were exposed to vehicle or VS-4718 (2.5 µM) for 1 h, later one of the microplates was irradiated with 6 Gy and then both microplates were incubated for 24 h at 37 °C and 5% CO2. Next, cells were fixed in cold methanol, membrane permeabilized using Triton X (Sigma, Germany) and incubated with Phospho-Histone H2AX (Ser139) Antibody #2577 at 1:100 dilution (Cell Signaling) and anti-Cytokeratin 8 antibodies #ab59400 at 1:100 dilution (Abcam) as primary antibodies. After which they were incubated with secondary antibodies, Alexa Fluor-488 goat anti-mouse and Alexa Fluor-546 goat anti-rabbit as 1:1000 dilution (Life Technologies, Thermo Fischer Inc., USA) and nuclear DNA was counterstained with DAPI. For cell cycle analysis, the microplates were coated with collagen type I (Collagen G, Biochrom Inc., Germany) before Panc‑1 cells were plated into wells as duplicates, to ensure activation of the β1-integrin-FAK axis [31], then treated with VS-4718 2.5 μM or vehicle for 24 h (exposure time for TKI in all radiation-sensitivity experiments). Later, cells were fixed with methanol at 20 °C and stained with DAPI to measure DNA content [32]. Both assays were assessed using high content microscopy (Olympus Scan*R, based on IX-81 inverted stage) and images were captured with a Hamamatsu Orca-ER and 20X UPLSAPO NA 0.75. Finally, the percentage of cancer cells with the number of foci above the basal average were represented in figures (Fig. 3 and suppl. Fig. 4a) as described before [33].
Collagen content
Stellate cells were incubated in vitro for 5 days either with VS-4718 (2.5 µm) or with the control (DMSO) in 24-well plates in triplicate, then were fixed and stained with Picro Sirius red to detect the collagen content in the cell culture. Each experiment was repeated three times.
After repeated washing to remove the nonbound stain, we examined the stained tissue by phase-contrast and fluorescence microscopy. Later, we dissolved the Picro Sirius red using NaOH and measured OD at 540 nm wavelength of the dissolved solution to quantify the difference of collagen content.
Three-dimensional multicellular tumor assay
We generated an in vitro multicellular three-dimensional (3D) tumor model using a standardized conical agarose microwell array (CAMA) platform as previously described [34]. CAMA allowed us to simultaneously generate hundreds of equally sized multicellular tumor aggregates for each matrix.
In short, agarose matrices were calibrated in cell culture medium 2 h before cell seeding. The cell suspension is used to seed an average of 50 cells per microwell; for coculture tumor spheroids the ratio of PSN‑1 and LTC-14 cells was 4:1, respectively. As a control, we also generated spheroids with only PSN‑1 and only LTC-14 (50 cells per microwell). The matrices containing tumor cells were incubated in 5% CO2 at 37 °C until they formed tumor aggregates on day 6, then they were treated with VS-4718 (2.5 μM) or vehicle. Then, 2 h later the matrices were irradiated with different doses (0–20 Gy, in 2 Gy gradient) in duplicate. The culture medium was replaced 24 h after the irradiation to wash out the treatment, and regularly every 3–4 days until day 28.
Volume-read out of tumor spheroids was done at day 6 and day 28 using a high-resolution scanner (CanoScan 9000F Mark II, Canon Inc.). Image processing and measuring the projected area of each cell aggregates was done using Image J software. We adopted spheroid control probability (SCP) and spheroid control dose 50 (SCD50) as analytical endpoints [35] on day 28. Cell aggregates were processed further for histology as previously described [34].
Statistical analysis
Graphs were generated using Microsoft Excel 2016 and GraphPad Prism. Statistical analysis was done using an unpaired t-test. Data are presented as mean ± standard deviation (SD). Statistical significance was mentioned as follows: *:P < 0.05, **:P < 0.001 and ***:P < 0.0001.
Results
Overexpression and genetic alterations of FAK in PDAC negatively impact outcome
First, we examined FAK differential expression between PDAC and normal tissue using GEPIA server. PTK2 expression was significantly higher in PDAC samples (n = 179) in comparison to the normal pancreatic tissue samples (n = 171) (Fig. 1a) and this overexpression was not significantly different across the four tumor stages (suppl. Fig. 1a).
Using cBioportal, we examined the TCGA, PanCancer atlas cohort, which included 168 pancreatic neoplasms samples with complete PTK2 mutations and expression data from a total of 184 patients originally in the cohort. Alterations in the PTK2 gene or its transcription were identified in 26% from PDAC samples (suppl. Fig. 1b).
The following alterations were identified: 15.48% high mRNA expression, 2.98% gene amplification, 0.6% mutation, deep deletion and low mRNA and 5.95% show multiple alterations. Amplification and gain were associated with the highest mRNA expression (suppl. Fig. 1c).
Using the survival data available from the cohort, we found that patients with any alteration in PTK2 gene had a significantly worse 3‑year progression-free survival and trend toward worse 3‑year survival in comparison to those without any PTK2 alteration (23.5 months vs not yet reached; p-value: 0.0264 and 17.9 vs 21.4 months; p-value: 0.0891, respectively; Fig. 1b,c).
Effect of VS-4718 on the expression of FAK and pFAK in pancreatic cell lines
Using GEMiCCL portal, we queried PTK2 in Panc‑1, PSN‑1 and MIA-PaCa 2. We did not find any PTK2 mutations or a difference in the expression or copy-number variations between the cell lines (suppl. Table 1).
Using immunoblotting, the basal level of total FAK was similar in all three pancreatic cancer cell lines. However, the basal levels of p‑FAK (Y397) varied among them, with PSN‑1 expressing the highest basal level (suppl. Fig. 2a).
Next, we examined the effect of a VS-4718 dose-gradient on p‑FAK. There was a clear dose-dependent inhibition of p‑FAK in two cell lines (Panc‑1 and PSN-1), with a marked reduction in the p‑FAK at 2.5 µM in Panc‑1 and PSN‑1 starting from 0.5 µM. Meanwhile, after an initial reduction of p‑FAK in MIAPaca‑2, the effect was almost constant throughout the dosage range from 50 nM to 5 µM. (Fig. 2a,b).
Effect of VS-4718 on cell growth of human pancreatic cancer cell lines
VS-4718 showed a dose-dependent reduction in all cell lines’ viability (Fig. 2c). The PSN‑1 cell line was the most sensitive cell line and the viability was significantly reduced with a longer exposure (24 h vs 48 h); the MIA PaCa 2 cell line again was the most resistant cell line to VS-4718. The IC50 doses for each cell line are shown in Table 1.
Using single-cell suspensions from the Panc‑1 cell line with LTC-14 cultured for 5 days either with VS-4718 or vehicle in microwells, we noticed that Panc‑1 single cells were not able to form colonies in VS-4718 microwells. In control microwells they were able to form colonies surrounded by the stromal cells (LTC-14), which emphasizes continuous FAK inhibition with longer exposure of cells to VS-4718 has proliferation-inhibitory effects on cancer cells (suppl. Fig. 2b).
VS-4718 in vitro sensitizes pancreatic cancer cells for radiation only as coculture with ECM-producing pancreatic stellate cells
While VS-4718 did not sensitize any of the three cell lines for radiation as a monoculture, using in vitro coculture set up for the assay by seeding single-cell suspensions from cancer cells on top of cultured human stellate cells or simultaneously with the LTC-14 immortalized stellate cells, VS-4718 had a significant effect on radiosensitization in at least two cell lines (Panc‑1 and PSN-1), and a statistically insignificant effect in the MIA PaCa 2 cell line (Fig. 3a).
To confirm that the radio-sensitizing mechanism of VS-4718 was attributed to FAK, we repeated the clonogenic assay using siRNA to knockdown PTK2. The results confirmed the same pattern of radio-sensitization only in the presence of stellate cells as an in vitro coculture (Fig. 3b). The radiosensitizing effect was similar using the h.PSCs or LTC-14 as stellate cells in the coculture (suppl. Fig. 3b,c) Also, the pharmacological FAK inhibition radiosensitized Panc‑1 on top of collagen I layer (suppl. Fig. 3d). The previous results emphasize the role of the ECM-producing stellate cells to stimulate FAK-derived survival in cancer cells and the interception of this axis would increase their radiosensitivity.
VS-4718 impaired DNA-repair in pancreatic cancer cells and arrested cell cycle
To examine how FAK inhibition could alter the radiosensitivity, we measured the level of residual γ‑H2AX foci in cancer cells 24 h after radiation which correlates with the impairment of DNA repair and radiosensitivity of the irradiated PCCs in coculture with PSCs. A level of 5–7 foci per nucleus was considered as the lower cut off, depending on the basal level of γ‑H2AX foci for cell lines as mentioned before [33].
We found a significant increase in the percentage of cancer cells having a significant number of foci using VS-4718 and radiotherapy in comparison to radiotherapy alone (Fig. 4). VS-4718 alone did not increase the number of γ‑H2AX foci, indicating that inhibiting FAK impaired DNA repair only (suppl. Fig. 4a).
Further, Panc‑1 exposure to 2.5 µM of VS-4718 for 24 h (inhibitor-exposure time of cell lines in radiation-sensitivity assays) resulted in cells arrest in the radiation-sensitive G2/M phase of the cell cycle 38.76 ± 7.53% vs. 10.16 ± 4.02% in control (DMSO) (suppl. Fig. 4b).
VS-4718 reduced the proliferation of tumor stromal cells and ECM production in vitro
We examined the impact of FAK inhibition using VS-4718 on stellate cells as the main component of the tumor microenvironment. VS-4718 reduced the viability of stellate cells (LTC-14) in a dose-dependent manner (Fig. 5a).
In addition, VS-4718 significantly inhibited the collagen production of stellate cells in vitro, as indicated by Picro Sirius red staining, under both phase contrast and fluorescence microscopy (Fig. 5b–g). We dissolved the attached staining and measured its OD to quantify this effect, which revealed a marked reduction of collagen content (Fig. 5h).
Combination of radiotherapy and VS-4718 effectively inhibited multicellular tumor spheroid growth
We examined the hypothesis of FAK modulation of radiation sensitivity in a 3D multicellular model (PSN-1 + LTC-14) that mimics to a greater extent a solid tumor compared to the 2‑dimensional monolayer coculture. VS-4718 alone reduced the size of spheroids but could not produce spheroid control (complete inhibition of spheroid growth; Fig. 6b). Also, irradiation alone resulted in the size reduction of the multicellular aggregates (Fig. 6b) but the spheroid control could not be achieved unless high doses were used. However, the additive effect of combined therapy effectively inhibited spheroid growth at lower radiation doses (Fig. 6d). We generated a spheroid control probability (SCP) curve vs. radiation dose, using a radiation dose range from 0 to 20 Gy (Fig. 6c). The tumor-stromal aggregates were completely controlled (100% nongrowing) after 20 Gy, this effect was achievable at half of the radiation dose (10 Gy) with the combined therapy. The SCD50 was 9.8 and 3.8 Gy for radiation alone and radiation + VS-4718, respectively, indicating the strong radiosensitizing effect of FAK inhibition in vitro in the 3D multicellular tumor model.
Discussion
PDAC represents a major challenge based on the scarcity of effective therapy. Local progression alone can be the leading cause of death in 30% of the patients [36]. The desmoplastic reaction and the interactions between tumor cells and ECM have been identified as being central to the process of PDAC progression and resistance to standard therapies [37]. Stellate cells are the primary source of ECM production in PDAC, being activated in carcinogenesis and showing increased alpha-SMA expression after chemoradiation [38], which may indicate a role in the therapy resistance of PDAC.
ECM induces integrin activation and assembly of focal adhesions with phosphorylation of FAK in cancer cells, which in turn potentiates the proliferation, stress survival, cancer stemness and migration [31]. While FAK has been considered lately valuable druggable target in cancer therapy, results from phase I studies mostly indicating stationary course as the best clinical outcome under FAK inhibitors [39]. This is important because it indicates that FAK inhibition would effectively work as a combination. Indeed, FAK inhibition has been found to improve the radiation sensitivity in HNSCC [40], KRAS mutant NSCLC [41], and glioblastoma multiforme [42].
In the current study, we found that FAK was significantly overexpressed in PDAC. Although its mutational incidence was very low, other genetic alterations, including mRNA expression, were high (26%), and this significantly reduced the progression free survival and may also reduce the overall survival in those patients. To our knowledge, we are the first to report this prognostic fact in PDAC.
For pharmacological FAK inhibition in this study, we used the commercially available tyrosine kinase inhibitor VS-4718, which is a highly selective reversible small-molecule FAK inhibitor. When using VS-4718 in the µM range, only FAK and FLT3 have negligible activity with the latter expressed only in cells of hematopoietic origin [43]. The IC50 of VS-4718 in pancreatic cancer cell lines ranged from 1.23 to 3.99 µM, whereby the response between the three cell lines to VS-4718 varied, which is in agreement with the findings of Shapiro et al., who reported fluctuations in IC50 of VS-4718 in different cell lines, having attributed it to the difference in Merlin’s expression which regulates FAK function [44], and also with findings in pediatric tumor cell lines [45]. The pharmacological inhibition of FAK could be improved further, especially in the MIA PaCa 2 cell line if higher doses were used. Furthermore, we used siRNA to knockdown PTK2 as a complementary method to alleviate the concerns that current results of VS-4718 could be due to off-target effects.
The presence of ECM-producing stellate cells in the milieu or ECM (collagen I) were an essential component that allowed the radiation-sensitizing effect of FAK inhibition to take place in a cell-line-dependent manner. These findings align with the findings from Mantoni et al. [46], who reported for the first time the potential radioprotective role of the stellate cells. Mantoni et al. attributed the effect to heterotypic signaling, which was found to be due to β1-integrin activation through ECM production. Also, our findings confirm data previously reported by Begum et al. about the role of a collagen I effect in the activation of FAK and intercepting this axis through pharmacological FAK inhibition resulted in a reduction of clonogenic PDAC growth and migration, and extended antitumor response to other therapeutics as gemcitabine and nab-paclitaxel [31]. Our study also indicates that FAK may be essential in DNA damage response and its pharmacological inhibition resulted in the maintenance of DNA damage response after radiation. Indeed, it has been reported that FAK-deficient breast cancer cells (PyMT) experienced arrest in cell growth and underwent apoptosis [47]. Further analysis revealed that the pharmacological FAK inhibition blocks the cell cycle in the G2/M phase, resulting in improved radiation sensitivity.
VS-4718 also effectively targeted the stellate cells, reduced their viability, and markedly reduced the ECM content in the culture, which would generally contribute to a better response of cancer cells to therapy. This has been previously reported using VS-4718 in PDAC in vivo resulting in the improvement of tumor response to checkpoint immunotherapy [20].
To address the shortcoming of not using an in vivo model, we subsequently adopted the CAMA platform to generate a multicellular (cancer and stellate cells) 3D model of PDAC. The pros of this platform include the following: the reproducible production of hundreds of tumor aggregates with a similar average number of cells and size; an in vitro 3D multicellular model that allows the examination of the relationship between specific components of the tumor microenvironment; and it acts as a standardized platform to test new therapies. Lastly, the conical design of the CAMA allows the multicellular tumor aggregates to grow as a single aggregate per microwell, independent from the spheroid-forming ability of the cancer cells [34]. The cons are mostly limited to the large design of the experiment using this platform to reach accurate readout, which would require a large number of matrices, cells and 6-wells plates, which limited our 3D-model experiment to only one cell line (PSN-1 with LTC-14).
We found the endpoints SCP and SCD50, mentioned by Ingargiola et al. [35], useful to evaluate the effect of therapy in our 3D spheroid model. We were able to scan the size of an average of 400 tumor aggregates per matrix along the radiation-dose gradient from 0–20 Gy in each arm of the experiment. That gave us more confidence about the interpretation of the entire experiment in comparison to other methods that allow the generation of only a few spheroids per experiment and where the spheroids are manually evaluated. In our hands, the inhibitory effect on the growth of the multicellular tumor aggregates between VS-4718 and radiation vs. radiation alone was undoubtedly strong, and SCD50 was 3.8 vs 9.8 Gy, respectively.
Taken together, pharmacological FAK-targeting strategies in PDAC, here VS-4718, may provide a therapeutic option for a certain subset of patients in the era of personalized therapy. Our experiments provide evidence for the in vitro radiosensitizing effect of VS-4718 in multicellular 2D and 3D models of PDAC. With the advancement of irradiation techniques in preclinical models, our data will open the door in the future to allow the evaluation of the radiosensitizing effect of FAK inhibition in vivo [48].
References
Jemal A et al (2009) Cancer Statistics, 2009. CA Cancer J Clin 59:225–249
Siegel RL, Miller KD, Jemal A (2015) Cancer statistics, 2015. CA Cancer J Clin 65:5–29. https://doi.org/10.3322/caac.21254
Erkan M et al (2008) The Activated Stroma Index is a novel and independent prognostic marker in pancreatic ductal adenocarcinoma. Clin Gastroenterol Hepatol 6:1155–1161
Netti PA, Berk DA, Swartz MA, Grodzinsky AJ, Jain RK (2000) Role of extracellular matrix assembly in interstitial transport in solid tumors. Cancer Res 60:2497–2503
Allam A et al (2017) Pancreatic stellate cells in pancreatic cancer: in focus. Pancreatology. https://doi.org/10.1016/j.pan.2017.05.390
Callery MP et al (2009) Pretreatment assessment of resectable and borderline resectable pancreatic cancer: expert consensus statement. Ann Surg Oncol 16:1727–1733
Cinar P, Ko AH (2014) Evolving treatment options for locally advanced unresectable pancreatic ductal adenocarcinoma. J Natl Compr Canc Netw 12:167–172
Chen Y, Sun X‑J, Jiang T‑H, Mao A‑W (2013) Combined radiochemotherapy in patients with locally advanced pancreatic cancer: a meta-analysis. World J Gastroenterol 19:7461–7471
Bachmayer S et al (2018) Nonmetastatic pancreatic cancer. Strahlenther Onkol 194:627–637
Gkika E et al (2017) Stereotactic body radiotherapy (SBRT) in recurrent or oligometastatic pancreatic cancer. Strahlenther Onkol 193:433–443
Mazzola R et al (2018) Linac-based stereotactic body radiation therapy for unresectable locally advanced pancreatic cancer: risk-adapted dose prescription and image-guided delivery. Strahlenther Onkol 194:835–842
Krishnan S et al (2016) Focal radiation therapy dose escalation improves overall survival in locally advanced pancreatic cancer patients receiving induction chemotherapy and consolidative chemoradiation. Int J Radiat Oncol 94:755–765
Hammel P et al (2016) Effect of chemoradiotherapy vs chemotherapy on survival in patients with locally advanced pancreatic cancer controlled after 4 months of Gemcitabine with or without Erlotinib. JAMA 315:1844
Schlaepfer DD, Hanks SK, Hunter T, van der Geer P (1994) Integrin-mediated signal transduction linked to Ras pathway by GRB2 binding to focal adhesion kinase. Nature 372:786–791
Cary LA, Chang JF, Guan JL (1996) Stimulation of cell migration by overexpression of focal adhesion kinase and its association with Src and Fyn. J Cell Sci 109(Pt7):1787–1794
Sieg DJ et al (2000) FAK integrates growth-factor and integrin signals to promote cell migration. Nat Cell Biol 2:249–256
Owens LV et al (1996) Focal adhesion kinase as a marker of invasive potential in differentiated human thyroid cancer. Ann Surg Oncol 3:100–105
Luo M et al (2009) Mammary epithelial-specific ablation of the focal adhesion kinase suppresses mammary tumorigenesis by affecting mammary cancer stem/progenitor cells. Cancer Res 69:466–474
Sulzmaier FJ, Jean C, Schlaepfer DD (2014) FAK in cancer: mechanistic findings and clinical applications. Nat Rev Cancer 14:598–610. https://doi.org/10.1038/nrc3792
Jiang H et al (2016) Targeting focal adhesion kinase renders pancreatic cancers responsive to checkpoint immunotherapy. Nat Med 22:851–860
Kolev VN et al (2017) Inhibition of FAK kinase activity preferentially targets cancer stem cells. Oncotarget 8:51733–51747
Tolosa EJ, Fernández-Zapico ME (2020) Targeting tumour microenvironment, a FAKtual challenge in pancreatic cancer. Gut Mon 69:1–2. https://doi.org/10.1136/gutjnl-2019-318962
Cerami E et al (2012) The cBio cancer genomics portal: an open platform for exploring multidimensional cancer genomics data. Cancer Discov 2:401–404
Gao J et al (2013) Integrative analysis of complex cancer genomics and clinical profiles using the cBioPortal. Sci Signal 6:pl1
Ardlie KG et al (2015) The Genotype-Tissue Expression (GTEx) pilot analysis: multitissue gene regulation in humans. Science 348:648–660
Aguirre AJ, Hruban RH, Raphael BJ (2017) Integrated Genomic Characterization of Pancreatic Ductal Adenocarcinoma. Cancer Cell. https://doi.org/10.1016/j.ccell.2017.07.007
Tang Z et al (2017) GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses. Web Serv 45(W1):W98–W102. https://doi.org/10.1093/nar/gkx247
Jeong I et al (2018) GEMiCCL: mining genotype and expression data of cancer cell lines with elaborate visualization. Database. https://doi.org/10.1093/database/bay041
Sparmann G et al (2004) Generation and characterization of immortalized rat pancreatic stellate cells. Am J Physiol – Gastrointest Liver Physiol 287(1):G211–9. https://doi.org/10.1152/ajpgi.00347.2003
Mahmood T, Yang P‑C (2012) Western blot: technique, theory, and trouble shooting. N Am J Med Sci 4:429–434
Begum A et al (2017) The extracellular matrix and focal adhesion kinase signaling regulate cancer stem cell function in pancreatic ductal adenocarcinoma. PLoS One. https://doi.org/10.1371/journal.pone.0180181
Roukos V, Pegoraro G, Voss TC, Misteli T (2015) Cell cycle staging of individual cells by fluorescence microscopy. Nat Protoc 10:334–348
Fokas E et al (2012) Targeting ATR in vivo using the novel inhibitor VE-822 results in selective sensitization of pancreatic tumors to radiation. Cell Death Dis 3:441
Thomsen AR et al (2018) A deep conical agarose microwell array for adhesion independent three-dimensional cell culture and dynamic volume measurement. Lab Chip 18:179–189
Ingargiola M et al (2014) Potential of a Cetuximab-based radioimmunotherapy combined with external irradiation manifests in a 3‑D cell assay. Int J Cancer. https://doi.org/10.1002/ijc.28735
Lloyd S, Chang BW (2013) New possibilities and potential benefits for local control in locally recurrent pancreatic cancer. J Gastrointest Oncol 4:340–342
Weniger M, Honselmann KC, Liss AS (2018) The extracellular matrix and pancreatic cancer: A complex relationship. Cancers 10(9):316. https://doi.org/10.3390/cancers10090316
Cabrera MC et al (2014) Human pancreatic cancer-associated stellate cells remain activated after in vivo chemoradiation. Front Oncol 4:102
de Jonge MJA et al (2019) Phase I study of BI 853520, an inhibitor of focal adhesion kinase, in patients with advanced or metastatic nonhematologic malignancies. Target Oncol 14:43–55
Eke I et al (2012) β 1 Integrin/FAK/cortactin signaling is essential for human head and neck cancer resistance to radiotherapy. J Clin Invest 122:1529–1540
Tang KJ et al (2016) Focal adhesion kinase regulates the DNA damage response and its inhibition radiosensitizes mutant KRAS lung cancer. Clin Cancer Res 22:5851–5863
Storch K, Sagerer A, Cordes N (2015) Cytotoxic and radiosensitizing effects of FAK targeting in human glioblastoma cells in vitro. Oncol Rep 33:2009–2016
Tanjoni I et al (2010) PND-1186 FAK inhibitor selectively promotes tumor cell apoptosis in three-dimensional environments. Cancer Biol Ther 9:762–775
Shapiro IM et al (2014) Merlin deficiency predicts FAK inhibitor sensitivity: a synthetic lethal relationship. Sci Transl Med 6:237ra68–237ra68
Kurmasheva RT et al (2017) Initial testing of VS-4718, a novel inhibitor of focal adhesion kinase (FAK), against pediatric tumor models by the Pediatric Preclinical Testing Program. Pediatr Blood Cancer. https://doi.org/10.1002/pbc.26304
Mantoni TS, Lunardi S, Al-Assar O, Masamune A, Brunner TB (2011) Pancreatic stellate cells radioprotect pancreatic cancer cells through β1-integrin signaling. Cancer Res 71:3453–3458
Pylayeva Y et al (2009) Ras- and PI3K-dependent breast tumorigenesis in mice and humans requires focal adhesion kinase signaling. J Clin Invest 119:252–266
Dobiasch S et al (2018) MRI-based high-precision irradiation in an orthotopic pancreatic tumor mouse model. Strahlentherapie Onkol 194:944–952
Acknowledgements
We thank Prof. Jörg Kleeff and his laboratory (Halle University, Germany) for providing us with human pancreatic stellate cells. Also, we would like to thank Christine Aldrian (Freiburg University, Germany) for the support through the experimental work.
Funding
Open Access funding provided by Projekt DEAL.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
A. A. Mohamed, A. Thomsen, M. Follo, C. Zamboglou, P. Bronsert, H. Mostafa, A. Amen, M. Mekawy, A.L. Grosu and T.B. Brunner declare that they have no competing interests.
Caption Electronic Supplementary Material
66_2020_1666_MOESM2_ESM.pdf
Suppl. Fig. 1: a PTK2 mRNA expression in PDAC among different tumor stages, (ANOVA test). b–c OncoPrint screens from CbioPortal illustrating the genetic alterations and expression heatmap in TCGA, PanCancer dataset. d represents PTK2 gene alterations versus mRNA expression from the dataset Pancreatic Adenocarcinoma (TCGA, PanCancer). PTK2 amplification and gain associated mostly with increase in mRNA expression. Suppl. Fig. 2: a Western blot of the basal expression of FAK and pFAK in 3 pancreatic cancer cell lines (n = 3). b Using single cell suspensions from the Panc‑1 cell line with LTC-14 cultured for 5 days either with VS-4718 or vehicle in microwells, cells were fixed, cancer cells labeled against cytokeratin 8. VS-4718 inhibited cell proliferation and Panc 1 cells couldn’t form colonies after 5 days of incubation (1), while they were able to proliferate and multiply in control microwells (2). PCCs were labeled against cytokeratin 8, then images were taken at 4x objective, scale bar = 500 μm. (not quantitively analyzed). Suppl. Fig. 3: a–d VS-4718 without stellate cells has no radio-sensitization effect at 6 Gy on Panc‑1 cell alone (a) the radio-sensitization effect appears only in co-culture with PSCs (no difference between h‑PSCs (b) or LTC-14 (C) ) also the effect appears by culturing Panc‑1 on top of collagen‑I layer (d). Altogether, FAK-inhibition block the survival stimulation of ECM to tumor cells. Data show as mean ± SD (n = 3; t-test; *P < 0.05) e Tumor cell colonies from a clonogenic survival assay with PCCs (Panc-1) in coculture with PSCs. Suppl. Fig. 4: a Effect VS-4718 on γ‑H2AX foci in 3 cancer lines in coculture with PSCs after 24h exposure (without irradiation), cells were fixed and stained for γ‑H2AX, cancer cells were labeled against cytokeratin 8, bars represent the percentage of cancer cells with more than 5 or 6 foci per nucleus. VS-4718 alone doesn’t induce increases in γ‑H2AX foci compared to control. b Treatment of Panc‑1 cells on collagen I layer with VS-4718 resulted in G2/M arrest. Summary of percentage of cells in each cell cycle phase after the treatment of Panc‑1 cells with VS-4718 for 24 h. Data is shown as the % of cells in G1, S, and G2‑M phases ± SD. (n = 3; *p <0.05)
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Mohamed, A.A., Thomsen, A., Follo, M. et al. FAK inhibition radiosensitizes pancreatic ductal adenocarcinoma cells in vitro. Strahlenther Onkol 197, 27–38 (2021). https://doi.org/10.1007/s00066-020-01666-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00066-020-01666-0