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Resolving domains of interdigitated phospholipid membranes with 95 GHz spin labeling EPR

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Abstract

High-field W-band (95 GHz) electron paramagnetic resonance (EPR) study of partitioning of a small nitroxide TEMPO (2,2,6,6-tetramethyl-1-piperidinyloxy) in multilamellar liposomes composed from 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) is described. The high-resolution spectra with a high signal-to-noise ratio were combined with automated least-squares simulation analysis to derive accurate partitioning coefficients of TEMPO in the membrane lipid phase and to follow the membrane phase transitions. The isotropic magnetic parameters, giso and Aiso were used to characterize the average polarity the spin label is experiencing in the membrane. We also report an empirical correlation between giso and Aiso for a set of protic and aprotic solvents and use this correlation to assign domains formed by interdigitation of DPPC bilayer under a high ethanol concentration of 1.2 M.

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References

  1. Borbat P.P., Costa-Filho A.J., Earle K.A., Moscicki J.K., Freed J.H.: Science291, 266–269 (2001)

    Article  ADS  Google Scholar 

  2. Hustedt E.J., Smirnov A.I., Laub C.F., Cobb C.E., Beth A.H.: Biophys. J.72, 1861–1877 (1997)

    Article  Google Scholar 

  3. Smirnova T.I., Smirnov A.I., Clarkson R.B., Belford R.L., Kotake Y., Janzen E.G.: J. Phys. Chem. B101, 3877–3885 (1997)

    Article  Google Scholar 

  4. Krinichnyi V.I., Grinberg O.Ya., Bogatyrenko V.R., Likhtenstein G.I., Lebedev Ya.: Biofizika30, 216–219 (1985)

    Google Scholar 

  5. Earle K.A., Moscicki J.K., Ge M., Budil D., Freed J.H.: Biophys. J.66, 1213–1221 (1994)

    Article  Google Scholar 

  6. Kawamura T., Matsunami S., Yonezawa T.: Bull. Chem. Soc. Jpn.40, 1111–1115 (1967)

    Article  Google Scholar 

  7. Griffith O.H., Dehlinger P.J., Van S.P.: J. Membr. Biol.15, 159–192 (1974)

    Article  Google Scholar 

  8. Ondar M.A., Griberg O.Ya., Dubinskii A.A., Lebedev Ya.S.: Sov. J. Chem. Phys.3, 781–792 (1985)

    Google Scholar 

  9. Steinhoff H.-J., Savitsky A., Wegener C., Pfeiffer M., Plato M., Möbius K.: Biochim. Biophys. Acta1457, 253–262 (2000)

    Article  Google Scholar 

  10. Krinichnyi V.I., Grinberg O.Ya., Yudanova E.I., Lyubashevskaya E.V., Antsiferova L.I., Likhtenstein G.I., Lebedev Ya.S.: Biofizika32, 215–220 (1984)

    Google Scholar 

  11. Smirnov A.I., Smirnova T.I., Morse P.D. II: Biophys. J.68, 2350–2360 (1995)

    Article  ADS  Google Scholar 

  12. Wang W., Belford R.L., Clarkson R.B., Davis P.H., Forrer J., Nilges M.J., Timken M.D., Watczak T., Thumauer M.C., Norris J.R., Morris A.L., Zwang Y.: Appl. Magn. Reson.6, 195–216 (1994)

    Google Scholar 

  13. Nilges M.J., Smirnov A.I., Clarkson R.B., Belford R.L.: Appl. Magn. Reson.16, 167–183 (1999)

    Article  Google Scholar 

  14. Smirnov A.I., Belford R.L.: J. Magn. Reson. A98, 65–73 (1995)

    Article  Google Scholar 

  15. Smirnov A.I., Clarkson R.B., Belford R.L.: J. Magn. Reson. B111, 149–157 (1996)

    Article  Google Scholar 

  16. Shimshick E.J., McConnell H.M.: Biochemistry12, 2351–2360 (1973)

    Article  Google Scholar 

  17. Chen S.C., Sturtevant J.M.: Biochemistry20, 713–718 (1981)

    Article  Google Scholar 

  18. Polnaszek C.F., Schreier S., Butler K.W., Smith I.C.P.: J. Am. Chem. Soc.100, 8223–8231 (1978)

    Article  Google Scholar 

  19. Stone A.J.: Proc. R. Soc. Lond. A271, 424–434 (1963)

    Article  ADS  MATH  Google Scholar 

  20. Barry J.A., Gawrisch K.: Biochemistry33, 8082–8088

  21. Chiou J.-S., Krishna P.R., Kamaya H., Ueda I.: Biochim. Biophys. Acta1100, 225–233 (1992)

    Google Scholar 

  22. Zeng J., Smith K.E., Chong P.L.-G.: Biophys. J.65, 1404–1414 (1993)

    Article  ADS  Google Scholar 

  23. Mou J., Yang J., Huang C., Shao Z.: Biochemistry33, 9981–9985 (1994)

    Article  Google Scholar 

  24. Chin J.H., Goldstein D.B.: Science196, 684–685 (1976)

    Article  ADS  Google Scholar 

Download references

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  1. Department of Chemistry, North Carolina State University, Raleigh, North Carolina, USA

    A. I. Smirnov & T. I. Smirnova

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  1. A. I. Smirnov
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  2. T. I. Smirnova
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Smirnov, A.I., Smirnova, T.I. Resolving domains of interdigitated phospholipid membranes with 95 GHz spin labeling EPR. Appl. Magn. Reson. 21, 453–467 (2001). https://doi.org/10.1007/BF03162420

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  • DOI: https://doi.org/10.1007/BF03162420

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