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High-frequencyAgrobacterium-med’iated genetic transformation of tongil rice varieties

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Abstract

We investigated the frequency of callus induction from mature-seed scutella of 39 varieties of Tongil rice(Oryza sativa japonica x indica). These were divided into two groups according to condition of the callus at the time of induction. Members of the first group, which developed brown calli, were further classified into two types (B1 or B2), based on the extent of their color change and callus condition. Tissues in the second group developed yellow calli, with varieties being placed into one of three types (Y1, Y2, or Y3) according to solidity. Type-Y1 seeds generated suitably soft calli that were healthy and grew rapidly. These calli were transformed with binary vector pGA2722 viaAgrobacterium- mediated co-cultivation. The vector contained the hygromycin-resistance selectable marker and agus reporter gene with an intron that is functional in plant cells but not inAgrobacterium. Most varieties that formed brown calli failed to generate transformants. Likewise, several varieties with suitably soft calli also failed. Among the 16 transformable varieties, the final regeneration frequencies ranged from 1.0 to 10.7%. The highest frequency was achieved by the variety Tongil, which also had the most rapidly growing calli. DNA gel-blot analysis revealed one to three copies of the introducedgus gene.

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Authors and Affiliations

  1. Department of Life Sciences, The Catholic University of Korea, 420-743, Puchon, Korea

    Min Chul Park, Junhye Shin, Naryung Kim, Hongjoo Cho & Seonghee Park

  2. National Research Laboratory of Plant Functional Genomics, Division of Molecular and Life Sciences, Pohang University of Science and Technology (POSTECH), 790-784, Pohang, Korea

    Kyungsook An, Sichul Lee & Gynheung An

Authors
  1. Min Chul Park
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  2. Junhye Shin
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  3. Naryung Kim
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  4. Hongjoo Cho
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  5. Seonghee Park
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  6. Kyungsook An
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  7. Sichul Lee
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  8. Gynheung An
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Correspondence to Gynheung An.

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Park, M.C., Shin, J., Kim, N. et al. High-frequencyAgrobacterium-med’iated genetic transformation of tongil rice varieties. J. Plant Biol. 46, 23–30 (2003). https://doi.org/10.1007/BF03030297

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  • DOI: https://doi.org/10.1007/BF03030297

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