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Binary vectors for efficient transformation of rice

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Abstract

We constructed binary vectors that were designed for transfer and expression of a gene into rice chromosomes. The binary vectors contained the hygromycin-resistance gene for selection of transformants and multiple-cloning sites within the transfer DNA. In addition, vectors were designed to express foreign genes using four kinds of promoters. We also report a procedure for efficient transformation of rice plants using scutellum-derived calli and theAgrobacterium strain LBA4404.

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Authors and Affiliations

  1. Department of Life Science and National Research Laboratory of Plant Functional Genomics, Pohang University of Science and Technology (POSTECH), 790-784, Pohang, Korea

    Sichul Lee, Jong -Seong Jeon, Ki -Hong Jung & Gynheung An

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  1. Sichul Lee
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  2. Jong -Seong Jeon
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  3. Ki -Hong Jung
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  4. Gynheung An
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Correspondence to Gynheung An.

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Lee, S., Jeon, J.S., Jung, K.H. et al. Binary vectors for efficient transformation of rice. J. Plant Biol. 42, 310–316 (1999). https://doi.org/10.1007/BF03030346

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  • DOI: https://doi.org/10.1007/BF03030346

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